Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves' orbitopathy.

<h4>Purpose</h4>The role of fibroblast growth factor (FGF) in orbital fibroblasts (OFs) is rarely known. In this study, we investigated the effect of FGF10 on fibrosis and the inflammation mechanism of Graves' orbitopathy (GO).<h4>Methods</h4>Orbital tissue from GO (n =...

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Autores principales:	Sun Young Jang, Soo Hyun Choi, Don Kikkawa, Eun Jig Lee, Jin Sook Yoon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c5f58a89724410bb727d210159c00f1
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spelling	oai:doaj.org-article:3c5f58a89724410bb727d210159c00f12021-12-02T20:18:19ZAssociation of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves' orbitopathy.1932-620310.1371/journal.pone.0255344https://doaj.org/article/3c5f58a89724410bb727d210159c00f12021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255344https://doaj.org/toc/1932-6203<h4>Purpose</h4>The role of fibroblast growth factor (FGF) in orbital fibroblasts (OFs) is rarely known. In this study, we investigated the effect of FGF10 on fibrosis and the inflammation mechanism of Graves' orbitopathy (GO).<h4>Methods</h4>Orbital tissue from GO (n = 15) and non-GO (n = 15) was obtained for this study. The mRNA and protein expression levels of FGF10 and FGF receptor 2b (FGFR2b) in orbital tissue were determined by real-time polymerase chain reaction, western blot analysis, and confocal microscopy. The effects of FGF10 on transforming growth factor (TGF)-β1 induced fibrotic proteins and interleukin (IL)-1β- or tumor necrosis factor (TNF)-α- induced inflammatory proteins were investigated using recombinant human (rh) FGF10 and small interfering (si) RNA transfection against FGF10.<h4>Results</h4>FGF10 and FGFR2b mRNA expression levels were significantly lower in GO orbital tissues than in non-GO orbital tissues (p = 0.009 and 0.005, respectively). Immunostaining of FGF10 in orbital adipose tissues showed differences in FGF10 expression between GO and control samples. Immunostaining of FGF10 was very weak in the orbital tissues of GO patients. TGF-β1-induced fibronectin, collagen Iα, α-smooth muscle actin protein expression in GO OFs was attenuated by rhFGF10 treatment and increased by knockdown of FGF10 via siFGF10 transfection. Similarly, IL-1β- or TNF-α-induced IL-6, IL-8, and cyclooxygenase-2 protein production in GO OFs was either blocked by rhFGF10 treatment or further upregulated by inhibition of FGF10 via siFGF10 transfection.<h4>Conclusions</h4>Our data demonstrate that FGF10 has beneficial effects on the inflammatory and fibrotic mechanisms of GO in primary cultured OFs, providing new insights into GO pathology and the discovery of FGF10 as a promising novel therapeutic application for the treatment of GO.Sun Young JangSoo Hyun ChoiDon KikkawaEun Jig LeeJin Sook YoonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255344 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sun Young Jang Soo Hyun Choi Don Kikkawa Eun Jig Lee Jin Sook Yoon Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves' orbitopathy.
description	<h4>Purpose</h4>The role of fibroblast growth factor (FGF) in orbital fibroblasts (OFs) is rarely known. In this study, we investigated the effect of FGF10 on fibrosis and the inflammation mechanism of Graves' orbitopathy (GO).<h4>Methods</h4>Orbital tissue from GO (n = 15) and non-GO (n = 15) was obtained for this study. The mRNA and protein expression levels of FGF10 and FGF receptor 2b (FGFR2b) in orbital tissue were determined by real-time polymerase chain reaction, western blot analysis, and confocal microscopy. The effects of FGF10 on transforming growth factor (TGF)-β1 induced fibrotic proteins and interleukin (IL)-1β- or tumor necrosis factor (TNF)-α- induced inflammatory proteins were investigated using recombinant human (rh) FGF10 and small interfering (si) RNA transfection against FGF10.<h4>Results</h4>FGF10 and FGFR2b mRNA expression levels were significantly lower in GO orbital tissues than in non-GO orbital tissues (p = 0.009 and 0.005, respectively). Immunostaining of FGF10 in orbital adipose tissues showed differences in FGF10 expression between GO and control samples. Immunostaining of FGF10 was very weak in the orbital tissues of GO patients. TGF-β1-induced fibronectin, collagen Iα, α-smooth muscle actin protein expression in GO OFs was attenuated by rhFGF10 treatment and increased by knockdown of FGF10 via siFGF10 transfection. Similarly, IL-1β- or TNF-α-induced IL-6, IL-8, and cyclooxygenase-2 protein production in GO OFs was either blocked by rhFGF10 treatment or further upregulated by inhibition of FGF10 via siFGF10 transfection.<h4>Conclusions</h4>Our data demonstrate that FGF10 has beneficial effects on the inflammatory and fibrotic mechanisms of GO in primary cultured OFs, providing new insights into GO pathology and the discovery of FGF10 as a promising novel therapeutic application for the treatment of GO.
format	article
author	Sun Young Jang Soo Hyun Choi Don Kikkawa Eun Jig Lee Jin Sook Yoon
author_facet	Sun Young Jang Soo Hyun Choi Don Kikkawa Eun Jig Lee Jin Sook Yoon
author_sort	Sun Young Jang
title	Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves' orbitopathy.
title_short	Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves' orbitopathy.
title_full	Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves' orbitopathy.
title_fullStr	Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves' orbitopathy.
title_full_unstemmed	Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves' orbitopathy.
title_sort	association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of graves' orbitopathy.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3c5f58a89724410bb727d210159c00f1
work_keys_str_mv	AT sunyoungjang associationoffibroblastgrowthfactor10withthefibroticandinflammatorypathogenesisofgravesorbitopathy AT soohyunchoi associationoffibroblastgrowthfactor10withthefibroticandinflammatorypathogenesisofgravesorbitopathy AT donkikkawa associationoffibroblastgrowthfactor10withthefibroticandinflammatorypathogenesisofgravesorbitopathy AT eunjiglee associationoffibroblastgrowthfactor10withthefibroticandinflammatorypathogenesisofgravesorbitopathy AT jinsookyoon associationoffibroblastgrowthfactor10withthefibroticandinflammatorypathogenesisofgravesorbitopathy
_version_	1718374278720651264

Association of fibroblast growth factor 10 with the fibrotic and inflammatory pathogenesis of Graves' orbitopathy.

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