Molecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from the Intensive Care Unit in a Tertiary Teaching Hospital in Malaysia

The emergence of carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) has now become a global sentinel event. CRAB infections often instigate severe clinical complications and are potentially fatal, especially for debilitated patients. The present study aimed to conduct molecular c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jia Jie Woon, Cindy Shuan Ju Teh, Chun Wie Chong, Kartini Abdul Jabar, Sasheela Ponnampalavanar, Nuryana Idris
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	carbapenem-resistant <i>Acinetobacter baumannii</i> intensive care unit endemicity Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/3c6452972a284b0b84a8d1eb0870912a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3c6452972a284b0b84a8d1eb0870912a
record_format	dspace
spelling	oai:doaj.org-article:3c6452972a284b0b84a8d1eb0870912a2021-11-25T16:23:10ZMolecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from the Intensive Care Unit in a Tertiary Teaching Hospital in Malaysia10.3390/antibiotics101113402079-6382https://doaj.org/article/3c6452972a284b0b84a8d1eb0870912a2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1340https://doaj.org/toc/2079-6382The emergence of carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) has now become a global sentinel event. CRAB infections often instigate severe clinical complications and are potentially fatal, especially for debilitated patients. The present study aimed to conduct molecular characterization on CRAB isolated from patients in the intensive care unit from 2015 to 2016 and determine the risk factors associated with patients’ mortality. One hundred CRAB isolates were retrospectively selected and included in this study. Antimicrobial susceptibility testing showed that all isolates remained susceptible to colistin, even though 62% of them conferred resistance to all other classes of antibiotics tested. OXA carbapenemase gene was found to be the predominant carbapenemase gene, with 99% of the isolates coharbouring <i>bla</i><sub>OXA-23</sub>-like and <i>bla</i><sub>OXA-51</sub>-like carbapenemase genes. All isolates were carrying intact <i>Car</i>O genes, with the presence of various degree of nucleotide insertion, deletion and substitution. Overall, PFGE subtyped the isolates into 13 distinct pulsotypes, with the presence of 2 predominant pulsotypes. Univariate analysis implied that age, infection/colonization by CRAB, ethnicity, comorbidity and CRAB specimen source were significantly associated with in-hospital mortality. Multivariate analysis identified a higher risk of mortality for patients who are of Chinese ethnicity with diabetes as an underlying disease. As CRAB infection could lead to high rate of mortality, comprehensive infection control measures are needed to minimize the spread of this pathogen.Jia Jie WoonCindy Shuan Ju TehChun Wie ChongKartini Abdul JabarSasheela PonnampalavanarNuryana IdrisMDPI AGarticlecarbapenem-resistant <i>Acinetobacter baumannii</i>intensive care unitendemicityTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1340, p 1340 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbapenem-resistant <i>Acinetobacter baumannii</i> intensive care unit endemicity Therapeutics. Pharmacology RM1-950
spellingShingle	carbapenem-resistant <i>Acinetobacter baumannii</i> intensive care unit endemicity Therapeutics. Pharmacology RM1-950 Jia Jie Woon Cindy Shuan Ju Teh Chun Wie Chong Kartini Abdul Jabar Sasheela Ponnampalavanar Nuryana Idris Molecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from the Intensive Care Unit in a Tertiary Teaching Hospital in Malaysia
description	The emergence of carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) has now become a global sentinel event. CRAB infections often instigate severe clinical complications and are potentially fatal, especially for debilitated patients. The present study aimed to conduct molecular characterization on CRAB isolated from patients in the intensive care unit from 2015 to 2016 and determine the risk factors associated with patients’ mortality. One hundred CRAB isolates were retrospectively selected and included in this study. Antimicrobial susceptibility testing showed that all isolates remained susceptible to colistin, even though 62% of them conferred resistance to all other classes of antibiotics tested. OXA carbapenemase gene was found to be the predominant carbapenemase gene, with 99% of the isolates coharbouring <i>bla</i><sub>OXA-23</sub>-like and <i>bla</i><sub>OXA-51</sub>-like carbapenemase genes. All isolates were carrying intact <i>Car</i>O genes, with the presence of various degree of nucleotide insertion, deletion and substitution. Overall, PFGE subtyped the isolates into 13 distinct pulsotypes, with the presence of 2 predominant pulsotypes. Univariate analysis implied that age, infection/colonization by CRAB, ethnicity, comorbidity and CRAB specimen source were significantly associated with in-hospital mortality. Multivariate analysis identified a higher risk of mortality for patients who are of Chinese ethnicity with diabetes as an underlying disease. As CRAB infection could lead to high rate of mortality, comprehensive infection control measures are needed to minimize the spread of this pathogen.
format	article
author	Jia Jie Woon Cindy Shuan Ju Teh Chun Wie Chong Kartini Abdul Jabar Sasheela Ponnampalavanar Nuryana Idris
author_facet	Jia Jie Woon Cindy Shuan Ju Teh Chun Wie Chong Kartini Abdul Jabar Sasheela Ponnampalavanar Nuryana Idris
author_sort	Jia Jie Woon
title	Molecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from the Intensive Care Unit in a Tertiary Teaching Hospital in Malaysia
title_short	Molecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from the Intensive Care Unit in a Tertiary Teaching Hospital in Malaysia
title_full	Molecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from the Intensive Care Unit in a Tertiary Teaching Hospital in Malaysia
title_fullStr	Molecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from the Intensive Care Unit in a Tertiary Teaching Hospital in Malaysia
title_full_unstemmed	Molecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from the Intensive Care Unit in a Tertiary Teaching Hospital in Malaysia
title_sort	molecular characterization of carbapenem-resistant <i>acinetobacter baumannii</i> isolated from the intensive care unit in a tertiary teaching hospital in malaysia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3c6452972a284b0b84a8d1eb0870912a
work_keys_str_mv	AT jiajiewoon molecularcharacterizationofcarbapenemresistantiacinetobacterbaumanniiiisolatedfromtheintensivecareunitinatertiaryteachinghospitalinmalaysia AT cindyshuanjuteh molecularcharacterizationofcarbapenemresistantiacinetobacterbaumanniiiisolatedfromtheintensivecareunitinatertiaryteachinghospitalinmalaysia AT chunwiechong molecularcharacterizationofcarbapenemresistantiacinetobacterbaumanniiiisolatedfromtheintensivecareunitinatertiaryteachinghospitalinmalaysia AT kartiniabduljabar molecularcharacterizationofcarbapenemresistantiacinetobacterbaumanniiiisolatedfromtheintensivecareunitinatertiaryteachinghospitalinmalaysia AT sasheelaponnampalavanar molecularcharacterizationofcarbapenemresistantiacinetobacterbaumanniiiisolatedfromtheintensivecareunitinatertiaryteachinghospitalinmalaysia AT nuryanaidris molecularcharacterizationofcarbapenemresistantiacinetobacterbaumanniiiisolatedfromtheintensivecareunitinatertiaryteachinghospitalinmalaysia
_version_	1718413193742647296

Molecular Characterization of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from the Intensive Care Unit in a Tertiary Teaching Hospital in Malaysia

Ejemplares similares