Dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease

Yabing Wang,1 Haoxuan Li,2 Yanhuizhi Feng,3 Peilin Jiang,2 Jiansheng Su,1 Chen Huang2 1Department of Prosthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China; 2Key Laboratory of Te...

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Autores principales:	Wang Y, Li H, Feng Y, Jiang P, Su J, Huang C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Periodontal disease controlled release drug-loaded micelles electrospun nanofibers scaffold Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3c67c700fef44491b5b3345ee4c35dab
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spelling	oai:doaj.org-article:3c67c700fef44491b5b3345ee4c35dab2021-12-02T02:21:41ZDual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease1178-2013https://doaj.org/article/3c67c700fef44491b5b3345ee4c35dab2019-02-01T00:00:00Zhttps://www.dovepress.com/dual-micelles-loaded-gelatin-nanofibers-and-their-application-in-lipop-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yabing Wang,1 Haoxuan Li,2 Yanhuizhi Feng,3 Peilin Jiang,2 Jiansheng Su,1 Chen Huang2 1Department of Prosthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China; 2Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; 3Department of Periodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China Introduction: Combined therapies utilizing inhibitors to remove pathogens are needed to suppress lipopolysaccharide (LPS)-induced periodontal disease. We prepared a novel, multi-agent delivery scaffold for periodontal treatment. Methods: In this study, we synthesized SP600125 (a JNK inhibitor) and SB203580 (a p38 inhibitor) drug-loaded poly(ethylene glycol)-block-caprolactone copolymer via dialysis method. The physical property of micelles was characterized through dynamic light scattering and transmission electron microscopy. The cell growth and LPS-induced MMP-2 and MMP-13 expression were evaluated through CCK-8, real-time PCR and Western blot assay. The release of SP600125 and SB203580 from different scaffolds was estimated. Microcomputed tomography and histology were used for evaluating the effect of the micelles-loaded nanofibers on the treatment of class II furcation defects in dogs. Results: The drug was then successfully incorporated into gelatin fibers during electrospinning process. We confirmed that the micelles had spherical structure and an average particle size of 160 nm for SP600125-micelles (SP-Ms) and 150 nm for SB203580-micelles (SB-Ms). The nanofiber scaffold showed excellent encapsulation capability, in vitro drug-release behavior, and cell compatibility. Real-time PCR and Western blot assay further indicated that LPS-induced MMP-2 and MMP-13 expression was significantly inhibited by the scaffold. Conclusion: The results suggested that the dual drug-loaded system developed in this study might become a highly effective therapy for periodontal disease. Keywords: periodontal disease, controlled release, drug-loaded micelles, electrospun nanofibers, scaffoldWang YLi HFeng YJiang PSu JHuang CDove Medical PressarticlePeriodontal diseasecontrolled releasedrug-loaded micelleselectrospun nanofibersscaffoldMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 963-976 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Periodontal disease controlled release drug-loaded micelles electrospun nanofibers scaffold Medicine (General) R5-920
spellingShingle	Periodontal disease controlled release drug-loaded micelles electrospun nanofibers scaffold Medicine (General) R5-920 Wang Y Li H Feng Y Jiang P Su J Huang C Dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease
description	Yabing Wang,1 Haoxuan Li,2 Yanhuizhi Feng,3 Peilin Jiang,2 Jiansheng Su,1 Chen Huang2 1Department of Prosthodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China; 2Key Laboratory of Textile Science & Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China; 3Department of Periodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China Introduction: Combined therapies utilizing inhibitors to remove pathogens are needed to suppress lipopolysaccharide (LPS)-induced periodontal disease. We prepared a novel, multi-agent delivery scaffold for periodontal treatment. Methods: In this study, we synthesized SP600125 (a JNK inhibitor) and SB203580 (a p38 inhibitor) drug-loaded poly(ethylene glycol)-block-caprolactone copolymer via dialysis method. The physical property of micelles was characterized through dynamic light scattering and transmission electron microscopy. The cell growth and LPS-induced MMP-2 and MMP-13 expression were evaluated through CCK-8, real-time PCR and Western blot assay. The release of SP600125 and SB203580 from different scaffolds was estimated. Microcomputed tomography and histology were used for evaluating the effect of the micelles-loaded nanofibers on the treatment of class II furcation defects in dogs. Results: The drug was then successfully incorporated into gelatin fibers during electrospinning process. We confirmed that the micelles had spherical structure and an average particle size of 160 nm for SP600125-micelles (SP-Ms) and 150 nm for SB203580-micelles (SB-Ms). The nanofiber scaffold showed excellent encapsulation capability, in vitro drug-release behavior, and cell compatibility. Real-time PCR and Western blot assay further indicated that LPS-induced MMP-2 and MMP-13 expression was significantly inhibited by the scaffold. Conclusion: The results suggested that the dual drug-loaded system developed in this study might become a highly effective therapy for periodontal disease. Keywords: periodontal disease, controlled release, drug-loaded micelles, electrospun nanofibers, scaffold
format	article
author	Wang Y Li H Feng Y Jiang P Su J Huang C
author_facet	Wang Y Li H Feng Y Jiang P Su J Huang C
author_sort	Wang Y
title	Dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease
title_short	Dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease
title_full	Dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease
title_fullStr	Dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease
title_full_unstemmed	Dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease
title_sort	dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/3c67c700fef44491b5b3345ee4c35dab
work_keys_str_mv	AT wangy dualmicellesloadedgelatinnanofibersandtheirapplicationinlipopolysaccharideinducedperiodontaldisease AT lih dualmicellesloadedgelatinnanofibersandtheirapplicationinlipopolysaccharideinducedperiodontaldisease AT fengy dualmicellesloadedgelatinnanofibersandtheirapplicationinlipopolysaccharideinducedperiodontaldisease AT jiangp dualmicellesloadedgelatinnanofibersandtheirapplicationinlipopolysaccharideinducedperiodontaldisease AT suj dualmicellesloadedgelatinnanofibersandtheirapplicationinlipopolysaccharideinducedperiodontaldisease AT huangc dualmicellesloadedgelatinnanofibersandtheirapplicationinlipopolysaccharideinducedperiodontaldisease
_version_	1718402520801345536

Dual micelles-loaded gelatin nanofibers and their application in lipopolysaccharide-induced periodontal disease

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