Improved DNA-Based Identification of Cervidae Species in Forensic Investigations

The main reasons for wildlife forensic research are animal poaching, illegal trade, and falsified game meat products. Small trace amounts, old and degraded materials present the most common samples in revealing criminal activities in this field. This is the reason why it is crucial to use adequate a...

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Autores principales:	Davitkov Dajana, Glavinić Uroš, Nešić Ksenija, Davitkov Darko, Vučićević Miloš, Nešić Vladimir, Stanimirović Zoran
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	cervidae species forensics molecular analysis poaching Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/3c696a2d4ce6498facfd1393a8e25733
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spelling	oai:doaj.org-article:3c696a2d4ce6498facfd1393a8e257332021-11-17T21:27:51ZImproved DNA-Based Identification of Cervidae Species in Forensic Investigations1820-744810.1515/acve-2017-0037https://doaj.org/article/3c696a2d4ce6498facfd1393a8e257332017-12-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0037https://doaj.org/toc/1820-7448The main reasons for wildlife forensic research are animal poaching, illegal trade, and falsified game meat products. Small trace amounts, old and degraded materials present the most common samples in revealing criminal activities in this field. This is the reason why it is crucial to use adequate and reliable methods and samples to identify animal species killed outside the hunting season or species protected by law. In this study, different endpoint PCR and real-time PCR protocols were compared in the identification of three Cervidae species (Capreolus capreolus, Cervus elaphus, Dama dama) from old and damaged material found in an enclosed area where the animals were kept. From a total of 129 samples, end point PCR provided results for 119 samples, while real-time PCR was successful in all cases. Also, we created and tested a protocol for simultaneous analyses of different types of samples, which is of great importance as when the amplification is carried out simultaneously it is more cost efficient and speeds up the process.Davitkov DajanaGlavinić UrošNešić KsenijaDavitkov DarkoVučićević MilošNešić VladimirStanimirović ZoranSciendoarticlecervidae speciesforensicsmolecular analysispoachingVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 4, Pp 449-458 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	cervidae species forensics molecular analysis poaching Veterinary medicine SF600-1100
spellingShingle	cervidae species forensics molecular analysis poaching Veterinary medicine SF600-1100 Davitkov Dajana Glavinić Uroš Nešić Ksenija Davitkov Darko Vučićević Miloš Nešić Vladimir Stanimirović Zoran Improved DNA-Based Identification of Cervidae Species in Forensic Investigations
description	The main reasons for wildlife forensic research are animal poaching, illegal trade, and falsified game meat products. Small trace amounts, old and degraded materials present the most common samples in revealing criminal activities in this field. This is the reason why it is crucial to use adequate and reliable methods and samples to identify animal species killed outside the hunting season or species protected by law. In this study, different endpoint PCR and real-time PCR protocols were compared in the identification of three Cervidae species (Capreolus capreolus, Cervus elaphus, Dama dama) from old and damaged material found in an enclosed area where the animals were kept. From a total of 129 samples, end point PCR provided results for 119 samples, while real-time PCR was successful in all cases. Also, we created and tested a protocol for simultaneous analyses of different types of samples, which is of great importance as when the amplification is carried out simultaneously it is more cost efficient and speeds up the process.
format	article
author	Davitkov Dajana Glavinić Uroš Nešić Ksenija Davitkov Darko Vučićević Miloš Nešić Vladimir Stanimirović Zoran
author_facet	Davitkov Dajana Glavinić Uroš Nešić Ksenija Davitkov Darko Vučićević Miloš Nešić Vladimir Stanimirović Zoran
author_sort	Davitkov Dajana
title	Improved DNA-Based Identification of Cervidae Species in Forensic Investigations
title_short	Improved DNA-Based Identification of Cervidae Species in Forensic Investigations
title_full	Improved DNA-Based Identification of Cervidae Species in Forensic Investigations
title_fullStr	Improved DNA-Based Identification of Cervidae Species in Forensic Investigations
title_full_unstemmed	Improved DNA-Based Identification of Cervidae Species in Forensic Investigations
title_sort	improved dna-based identification of cervidae species in forensic investigations
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/3c696a2d4ce6498facfd1393a8e25733
work_keys_str_mv	AT davitkovdajana improveddnabasedidentificationofcervidaespeciesinforensicinvestigations AT glavinicuros improveddnabasedidentificationofcervidaespeciesinforensicinvestigations AT nesicksenija improveddnabasedidentificationofcervidaespeciesinforensicinvestigations AT davitkovdarko improveddnabasedidentificationofcervidaespeciesinforensicinvestigations AT vucicevicmilos improveddnabasedidentificationofcervidaespeciesinforensicinvestigations AT nesicvladimir improveddnabasedidentificationofcervidaespeciesinforensicinvestigations AT stanimiroviczoran improveddnabasedidentificationofcervidaespeciesinforensicinvestigations
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Improved DNA-Based Identification of Cervidae Species in Forensic Investigations

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