Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle.

Cytokinins (CKs) plays a key role in plant adaptation over a range of different stress conditions. Here, we analyze the effects of a cytokinin (i.e., kinetin, KN) on the growth, photosynthesis (rate of O2 evolution), PS II photochemistry and AsA-GSH cycle in Trigonella seedlings grown under cadmium...

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Autores principales:	Gausiya Bashri, Shikha Singh, Sheo Mohan Prasad, Mohammad Javed Ansari, Salma Usmani, Saleh Alfarraj, Sulaiman Ali Alharbi, Marian Brestic
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c6ba7d560d34a88b83c13e0dbc32ef5
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spelling	oai:doaj.org-article:3c6ba7d560d34a88b83c13e0dbc32ef52021-12-02T20:10:17ZKinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle.1932-620310.1371/journal.pone.0249230https://doaj.org/article/3c6ba7d560d34a88b83c13e0dbc32ef52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0249230https://doaj.org/toc/1932-6203Cytokinins (CKs) plays a key role in plant adaptation over a range of different stress conditions. Here, we analyze the effects of a cytokinin (i.e., kinetin, KN) on the growth, photosynthesis (rate of O2 evolution), PS II photochemistry and AsA-GSH cycle in Trigonella seedlings grown under cadmium (Cd) stress. Trigonella seeds were sown in soil amended with 0, 3 and 9 mg Cd kg-1 soil, and after 15 days resultant seedlings were sprayed with three doses of KN, i.e.,10 μM (low, KNL), 50 μM (medium, KNM) and 100 μM (high, KNH); subsequent experiments were performed after 15 days of KN application, i.e., 30 days after sowing. Cadmium toxicity induced oxidative damage as shown by decreased seedling growth and photosynthetic pigment production (Chl a, Chl b and Car), rates of O2-evolution, and photochemistry of PS II of Trigonella seedlings, all accompanied by an increase in H2O2 accumulation. Supplementation with doses of KN at KNL and KNM significantly improved the growth and photosynthetic activity by reducing H2O2 accumulation through the up-regulation AsA-GSH cycle. Notably, KNL and KNM doses stimulated the rate of enzyme activities of APX, GR and DHAR, involved in the AsA-GSH cycle thereby efficiently regulates the level of AsA and GSH in Trigonella grown under Cd stress. The study concludes that KN can mitigate the damaging effects of Cd stress on plant growth by maintaining the redox status (>ratios: AsA/DHA and GSH/GSSG) of cells through the regulation of AsA-GSH cycle at 10 and 50 μM KN under Cd stress conditions. At 100 μM KN, the down-regulation of AsA-GSH cycle did not support the growth and PS II activity of the test seedlings.Gausiya BashriShikha SinghSheo Mohan PrasadMohammad Javed AnsariSalma UsmaniSaleh AlfarrajSulaiman Ali AlharbiMarian BresticPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0249230 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gausiya Bashri Shikha Singh Sheo Mohan Prasad Mohammad Javed Ansari Salma Usmani Saleh Alfarraj Sulaiman Ali Alharbi Marian Brestic Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle.
description	Cytokinins (CKs) plays a key role in plant adaptation over a range of different stress conditions. Here, we analyze the effects of a cytokinin (i.e., kinetin, KN) on the growth, photosynthesis (rate of O2 evolution), PS II photochemistry and AsA-GSH cycle in Trigonella seedlings grown under cadmium (Cd) stress. Trigonella seeds were sown in soil amended with 0, 3 and 9 mg Cd kg-1 soil, and after 15 days resultant seedlings were sprayed with three doses of KN, i.e.,10 μM (low, KNL), 50 μM (medium, KNM) and 100 μM (high, KNH); subsequent experiments were performed after 15 days of KN application, i.e., 30 days after sowing. Cadmium toxicity induced oxidative damage as shown by decreased seedling growth and photosynthetic pigment production (Chl a, Chl b and Car), rates of O2-evolution, and photochemistry of PS II of Trigonella seedlings, all accompanied by an increase in H2O2 accumulation. Supplementation with doses of KN at KNL and KNM significantly improved the growth and photosynthetic activity by reducing H2O2 accumulation through the up-regulation AsA-GSH cycle. Notably, KNL and KNM doses stimulated the rate of enzyme activities of APX, GR and DHAR, involved in the AsA-GSH cycle thereby efficiently regulates the level of AsA and GSH in Trigonella grown under Cd stress. The study concludes that KN can mitigate the damaging effects of Cd stress on plant growth by maintaining the redox status (>ratios: AsA/DHA and GSH/GSSG) of cells through the regulation of AsA-GSH cycle at 10 and 50 μM KN under Cd stress conditions. At 100 μM KN, the down-regulation of AsA-GSH cycle did not support the growth and PS II activity of the test seedlings.
format	article
author	Gausiya Bashri Shikha Singh Sheo Mohan Prasad Mohammad Javed Ansari Salma Usmani Saleh Alfarraj Sulaiman Ali Alharbi Marian Brestic
author_facet	Gausiya Bashri Shikha Singh Sheo Mohan Prasad Mohammad Javed Ansari Salma Usmani Saleh Alfarraj Sulaiman Ali Alharbi Marian Brestic
author_sort	Gausiya Bashri
title	Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle.
title_short	Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle.
title_full	Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle.
title_fullStr	Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle.
title_full_unstemmed	Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle.
title_sort	kinetin mitigates cd-induced damagesto growth, photosynthesis and ps ii photochemistry of trigonella seedlings by up-regulating ascorbate-glutathione cycle.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3c6ba7d560d34a88b83c13e0dbc32ef5
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Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle.

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