Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630

Abstract The pandemic of 2019 caused by the novel coronavirus (SARS-CoV-2) is still rapidly spreading worldwide. Nucleic acid amplification serves as the gold standard method for confirmation of COVID-19 infection. However, challenges faced for diagnostic laboratories from undeveloped countries incl...

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Autores principales:	Alejandro Castellanos-Gonzalez, Thomas R. Shelite, Nicole Lloyd, Aygul Sadiqova, Ren Ping, Natalie Williams-Bouyer, Peter C. Melby, Bruno L. Travi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c7d2cb5aef941e292e49bff2783e8c4
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spelling	oai:doaj.org-article:3c7d2cb5aef941e292e49bff2783e8c42021-12-02T15:23:17ZDirect RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-63010.1038/s41598-021-93333-22045-2322https://doaj.org/article/3c7d2cb5aef941e292e49bff2783e8c42021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93333-2https://doaj.org/toc/2045-2322Abstract The pandemic of 2019 caused by the novel coronavirus (SARS-CoV-2) is still rapidly spreading worldwide. Nucleic acid amplification serves as the gold standard method for confirmation of COVID-19 infection. However, challenges faced for diagnostic laboratories from undeveloped countries includes shortage of kits and supplies to purify viral RNA. Therefore, it is urgent to validate alternative nucleic acid isolation methods for SARS-CoV-2. Our results demonstrate that a concentrated viral lysis amplification buffer (vLAB) prepared with the nonionic detergent IGEPAL enables qualitative detection of SARS-CoV-2 by direct Reverse Transcriptase-Polymerase Chain Reaction (dRT-PCR). Furthermore, vLAB was effective in inactivating SARS-CoV-2. Since this method is inexpensive and no RNA purification equipment or additional cDNA synthesis is required, this dRT-PCR with vLAB should be considered as an alternative method for qualitative detection of SARS-CoV-2.Alejandro Castellanos-GonzalezThomas R. SheliteNicole LloydAygul SadiqovaRen PingNatalie Williams-BouyerPeter C. MelbyBruno L. TraviNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alejandro Castellanos-Gonzalez Thomas R. Shelite Nicole Lloyd Aygul Sadiqova Ren Ping Natalie Williams-Bouyer Peter C. Melby Bruno L. Travi Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630
description	Abstract The pandemic of 2019 caused by the novel coronavirus (SARS-CoV-2) is still rapidly spreading worldwide. Nucleic acid amplification serves as the gold standard method for confirmation of COVID-19 infection. However, challenges faced for diagnostic laboratories from undeveloped countries includes shortage of kits and supplies to purify viral RNA. Therefore, it is urgent to validate alternative nucleic acid isolation methods for SARS-CoV-2. Our results demonstrate that a concentrated viral lysis amplification buffer (vLAB) prepared with the nonionic detergent IGEPAL enables qualitative detection of SARS-CoV-2 by direct Reverse Transcriptase-Polymerase Chain Reaction (dRT-PCR). Furthermore, vLAB was effective in inactivating SARS-CoV-2. Since this method is inexpensive and no RNA purification equipment or additional cDNA synthesis is required, this dRT-PCR with vLAB should be considered as an alternative method for qualitative detection of SARS-CoV-2.
format	article
author	Alejandro Castellanos-Gonzalez Thomas R. Shelite Nicole Lloyd Aygul Sadiqova Ren Ping Natalie Williams-Bouyer Peter C. Melby Bruno L. Travi
author_facet	Alejandro Castellanos-Gonzalez Thomas R. Shelite Nicole Lloyd Aygul Sadiqova Ren Ping Natalie Williams-Bouyer Peter C. Melby Bruno L. Travi
author_sort	Alejandro Castellanos-Gonzalez
title	Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630
title_short	Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630
title_full	Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630
title_fullStr	Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630
title_full_unstemmed	Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630
title_sort	direct rt-pcr amplification of sars-cov-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with igepal-630
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3c7d2cb5aef941e292e49bff2783e8c4
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Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630

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