MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3.
MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells in culture. In this study we demonstrate that miR-7 targets key regulators of the G1 to S phase transition, including Skp2 and Psme3, to promote increased levels of p27(KIP) and temporary growth arrest of CHO...
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Public Library of Science (PLoS)
2013
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oai:doaj.org-article:3c7d73595041443e97e7b24bd5130b882021-11-18T07:42:44ZMiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3.1932-620310.1371/journal.pone.0065671https://doaj.org/article/3c7d73595041443e97e7b24bd5130b882013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23762407/?tool=EBIhttps://doaj.org/toc/1932-6203MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells in culture. In this study we demonstrate that miR-7 targets key regulators of the G1 to S phase transition, including Skp2 and Psme3, to promote increased levels of p27(KIP) and temporary growth arrest of CHO cells in the G1 phase. Simultaneously, the down-regulation of DNA repair-specific proteins via miR-7 including Rad54L, and pro-apoptotic regulators such as p53, combined with the up-regulation of anti-apoptotic factors like p-Akt, promoted cell survival while arrested in G1. Thus miR-7 can co-ordinate the levels of multiple genes and proteins to influence G1 to S phase transition and the apoptotic response in order to maintain cellular homeostasis. This work provides further mechanistic insight into the role of miR-7 as a regulator of cell growth in times of cellular stress.Noelia SanchezMark GallagherNga LaoClair GallagherColin ClarkePadraig DoolanSinead AherneAlfonso BlancoPaula MeleadyMartin ClynesNiall BarronPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65671 (2013) |
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Medicine R Science Q |
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Medicine R Science Q Noelia Sanchez Mark Gallagher Nga Lao Clair Gallagher Colin Clarke Padraig Doolan Sinead Aherne Alfonso Blanco Paula Meleady Martin Clynes Niall Barron MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3. |
| description |
MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells in culture. In this study we demonstrate that miR-7 targets key regulators of the G1 to S phase transition, including Skp2 and Psme3, to promote increased levels of p27(KIP) and temporary growth arrest of CHO cells in the G1 phase. Simultaneously, the down-regulation of DNA repair-specific proteins via miR-7 including Rad54L, and pro-apoptotic regulators such as p53, combined with the up-regulation of anti-apoptotic factors like p-Akt, promoted cell survival while arrested in G1. Thus miR-7 can co-ordinate the levels of multiple genes and proteins to influence G1 to S phase transition and the apoptotic response in order to maintain cellular homeostasis. This work provides further mechanistic insight into the role of miR-7 as a regulator of cell growth in times of cellular stress. |
| format |
article |
| author |
Noelia Sanchez Mark Gallagher Nga Lao Clair Gallagher Colin Clarke Padraig Doolan Sinead Aherne Alfonso Blanco Paula Meleady Martin Clynes Niall Barron |
| author_facet |
Noelia Sanchez Mark Gallagher Nga Lao Clair Gallagher Colin Clarke Padraig Doolan Sinead Aherne Alfonso Blanco Paula Meleady Martin Clynes Niall Barron |
| author_sort |
Noelia Sanchez |
| title |
MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3. |
| title_short |
MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3. |
| title_full |
MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3. |
| title_fullStr |
MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3. |
| title_full_unstemmed |
MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3. |
| title_sort |
mir-7 triggers cell cycle arrest at the g1/s transition by targeting multiple genes including skp2 and psme3. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/3c7d73595041443e97e7b24bd5130b88 |
| work_keys_str_mv |
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| _version_ |
1718423091073253376 |