Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s

The type VI secretion system (T6SS) is a new secretion system that is widely distributed among Gram-negative bacteria. The core component hemolysin-coregulated protein (Hcp) can be used as both its structural protein and secretory protein or chaperone protein. Studies on Hcp are important to elucida...

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Autores principales:	Ping Wang, Jun-Fang Dong, Ren-Qing Li, Lei Li, Qing-Hua Zou
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	salmonella typhimurium type vi secretion system(t6ss) hemolysin-coregulatory protein (hcp) virulence flagella Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/3c7feb24d5d649f992b63912f5bf494a
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spelling	oai:doaj.org-article:3c7feb24d5d649f992b63912f5bf494a2021-11-17T14:21:59ZRoles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s2150-55942150-560810.1080/21505594.2020.1854538https://doaj.org/article/3c7feb24d5d649f992b63912f5bf494a2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1854538https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608The type VI secretion system (T6SS) is a new secretion system that is widely distributed among Gram-negative bacteria. The core component hemolysin-coregulated protein (Hcp) can be used as both its structural protein and secretory protein or chaperone protein. Studies on Hcp are important to elucidate the overall virulence mechanism of T6SS. Salmonella typhimurium is an important foodborne pathogen. There are three copies of hcp genes identified in S. Typhimurium 14028s. This study aimed to characterize the functions of the three Hcp family proteins and to elucidate the interactions among them. The hcp gene deletion mutants were constructed by λ Red-based recombination system. Effects of hcp mutation on the pathogenicity of 14028s were studied by bacterial competition assays, Dictyostelium discoideum assays and mouse model. The three Hcp family proteins were found to play different roles. Hcp1 can affect the transcription of rpoS and type 2 flagellar gene and influence the motility of 14028s. It is also involved in the intracellular survival of 14028s in Dictyostelium discoideum; Hcp2 is involved in the early proliferative capacity of 14028s in mice and can prevent its excessive proliferation; Hcp3 did not show direct functions in these assays. Hcp1 can interact with Hcp2 and Hcp3. Deletion of one hcp gene can result in a transcription level variation in the other two hcp genes. Our findings elucidated the functions of the three Hcp family proteins in S.Typhimurium and illustrated that there are interactions between different Hcp proteins. This study will be helpful to fully understand how T6SS actions in an organism.Ping WangJun-Fang DongRen-Qing LiLei LiQing-Hua ZouTaylor & Francis Grouparticlesalmonella typhimuriumtype vi secretion system(t6ss)hemolysin-coregulatory protein (hcp)virulenceflagellaInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1716-1726 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	salmonella typhimurium type vi secretion system(t6ss) hemolysin-coregulatory protein (hcp) virulence flagella Infectious and parasitic diseases RC109-216
spellingShingle	salmonella typhimurium type vi secretion system(t6ss) hemolysin-coregulatory protein (hcp) virulence flagella Infectious and parasitic diseases RC109-216 Ping Wang Jun-Fang Dong Ren-Qing Li Lei Li Qing-Hua Zou Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s
description	The type VI secretion system (T6SS) is a new secretion system that is widely distributed among Gram-negative bacteria. The core component hemolysin-coregulated protein (Hcp) can be used as both its structural protein and secretory protein or chaperone protein. Studies on Hcp are important to elucidate the overall virulence mechanism of T6SS. Salmonella typhimurium is an important foodborne pathogen. There are three copies of hcp genes identified in S. Typhimurium 14028s. This study aimed to characterize the functions of the three Hcp family proteins and to elucidate the interactions among them. The hcp gene deletion mutants were constructed by λ Red-based recombination system. Effects of hcp mutation on the pathogenicity of 14028s were studied by bacterial competition assays, Dictyostelium discoideum assays and mouse model. The three Hcp family proteins were found to play different roles. Hcp1 can affect the transcription of rpoS and type 2 flagellar gene and influence the motility of 14028s. It is also involved in the intracellular survival of 14028s in Dictyostelium discoideum; Hcp2 is involved in the early proliferative capacity of 14028s in mice and can prevent its excessive proliferation; Hcp3 did not show direct functions in these assays. Hcp1 can interact with Hcp2 and Hcp3. Deletion of one hcp gene can result in a transcription level variation in the other two hcp genes. Our findings elucidated the functions of the three Hcp family proteins in S.Typhimurium and illustrated that there are interactions between different Hcp proteins. This study will be helpful to fully understand how T6SS actions in an organism.
format	article
author	Ping Wang Jun-Fang Dong Ren-Qing Li Lei Li Qing-Hua Zou
author_facet	Ping Wang Jun-Fang Dong Ren-Qing Li Lei Li Qing-Hua Zou
author_sort	Ping Wang
title	Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s
title_short	Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s
title_full	Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s
title_fullStr	Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s
title_full_unstemmed	Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s
title_sort	roles of the hcp family proteins in the pathogenicity of salmonella typhimurium 14028s
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/3c7feb24d5d649f992b63912f5bf494a
work_keys_str_mv	AT pingwang rolesofthehcpfamilyproteinsinthepathogenicityofsalmonellatyphimurium14028s AT junfangdong rolesofthehcpfamilyproteinsinthepathogenicityofsalmonellatyphimurium14028s AT renqingli rolesofthehcpfamilyproteinsinthepathogenicityofsalmonellatyphimurium14028s AT leili rolesofthehcpfamilyproteinsinthepathogenicityofsalmonellatyphimurium14028s AT qinghuazou rolesofthehcpfamilyproteinsinthepathogenicityofsalmonellatyphimurium14028s
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Roles of the Hcp family proteins in the pathogenicity of Salmonella typhimurium 14028s

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