AFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study

Barbara Ruozi1, Daniela Belletti1, Andrea Tombesi2, Giovanni Tosi1, Lucia Bondioli1, Flavio Forni1, Maria Angela Vandelli11Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Modena, Italy; 2C.I.G.S., University of Modena and Reggio Emilia, Modena, ItalyAbstract: An outsta...

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Autores principales:	Barbara Ruozi, Daniela Belletti, Andrea Tombesi, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3c823a3b5372401dbd6471a030b15cf4
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spelling	oai:doaj.org-article:3c823a3b5372401dbd6471a030b15cf42021-12-02T01:50:39ZAFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study1176-91141178-2013https://doaj.org/article/3c823a3b5372401dbd6471a030b15cf42011-03-01T00:00:00Zhttp://www.dovepress.com/afm-esem-tem-and-clsm-in-liposomal-characterization-a-comparative-stud-a6689https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Barbara Ruozi1, Daniela Belletti1, Andrea Tombesi2, Giovanni Tosi1, Lucia Bondioli1, Flavio Forni1, Maria Angela Vandelli11Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Modena, Italy; 2C.I.G.S., University of Modena and Reggio Emilia, Modena, ItalyAbstract: An outstanding aspect of pharmaceutical nanotechnology lies in the characterization of nanocarriers for targeting of drugs and other bioactive agents. The development of microscopic techniques has made the study of the surface and systems architecture more attractive. In the field of pharmaceutical nanosystems, researchers have collected vital information on size, stability, and bilayer organization through the microscopic characterization of liposomes. This paper aims to compare the results obtained by atomic force microscopy, environmental scanning electron microscopy, transmission electron microscopy, and confocal laser scanning microscopy to point out the limits and advantages of these applications in the evaluation of vesicular systems. Besides this comparative aim, our work proposes a simple confocal laser scanning microscopy procedure to rapidly and easily detect the liposomal membrane.Keywords: atomic force microscopy, transmission electron microscopy, environmental scanning electron microscopy, confocal laser scanning microscopy Barbara RuoziDaniela BellettiAndrea Tombesiet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 557-563 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Barbara Ruozi Daniela Belletti Andrea Tombesi et al AFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study
description	Barbara Ruozi1, Daniela Belletti1, Andrea Tombesi2, Giovanni Tosi1, Lucia Bondioli1, Flavio Forni1, Maria Angela Vandelli11Department of Pharmaceutical Sciences, University of Modena and Reggio Emilia, Modena, Italy; 2C.I.G.S., University of Modena and Reggio Emilia, Modena, ItalyAbstract: An outstanding aspect of pharmaceutical nanotechnology lies in the characterization of nanocarriers for targeting of drugs and other bioactive agents. The development of microscopic techniques has made the study of the surface and systems architecture more attractive. In the field of pharmaceutical nanosystems, researchers have collected vital information on size, stability, and bilayer organization through the microscopic characterization of liposomes. This paper aims to compare the results obtained by atomic force microscopy, environmental scanning electron microscopy, transmission electron microscopy, and confocal laser scanning microscopy to point out the limits and advantages of these applications in the evaluation of vesicular systems. Besides this comparative aim, our work proposes a simple confocal laser scanning microscopy procedure to rapidly and easily detect the liposomal membrane.Keywords: atomic force microscopy, transmission electron microscopy, environmental scanning electron microscopy, confocal laser scanning microscopy
format	article
author	Barbara Ruozi Daniela Belletti Andrea Tombesi et al
author_facet	Barbara Ruozi Daniela Belletti Andrea Tombesi et al
author_sort	Barbara Ruozi
title	AFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study
title_short	AFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study
title_full	AFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study
title_fullStr	AFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study
title_full_unstemmed	AFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study
title_sort	afm, esem, tem, and clsm in liposomal characterization: a comparative study
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/3c823a3b5372401dbd6471a030b15cf4
work_keys_str_mv	AT barbararuozi afmesemtemandclsminliposomalcharacterizationacomparativestudy AT danielabelletti afmesemtemandclsminliposomalcharacterizationacomparativestudy AT andreatombesi afmesemtemandclsminliposomalcharacterizationacomparativestudy AT etal afmesemtemandclsminliposomalcharacterizationacomparativestudy
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AFM, ESEM, TEM, and CLSM in liposomal characterization: a comparative study

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