Prevalence of <i>Borrelia burgdorferi sensu</i> lato genetic markers in blood-sucking ticks in suburban park zones in Saint Petersburg

Tick-borne bacterial and viral infections are widespread in middle latitudes of the Northern hemisphere. Natural foci of such infections coincide with geographic areas inhabited by ixodid ticks. Ixodid tick-borne borreliosis is a pressing issue for some territories of Russia, especially for the Nort...

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Autores principales:	Yu. A. Panferova, A. N. Vaganova, O. A. Freylikhman, K. A. Tretyakov, S. G. Medvedev, A. O. Shapar’, N. K. Tokarevich
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2020
Materias:	ixodid tick-borne borreliosis borrelia genospecies pcr prevalence Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/3c86cd723b3042bd92c143e5efa53155
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spelling	oai:doaj.org-article:3c86cd723b3042bd92c143e5efa531552021-11-22T07:09:54ZPrevalence of <i>Borrelia burgdorferi sensu</i> lato genetic markers in blood-sucking ticks in suburban park zones in Saint Petersburg2220-76192313-739810.15789/2220-7619-POB-806https://doaj.org/article/3c86cd723b3042bd92c143e5efa531552020-04-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/806https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Tick-borne bacterial and viral infections are widespread in middle latitudes of the Northern hemisphere. Natural foci of such infections coincide with geographic areas inhabited by ixodid ticks. Ixodid tick-borne borreliosis is a pressing issue for some territories of Russia, especially for the North-Western Federal District and St. Petersburg megalopolis as well as adjacent areas of the Leningrad District, where people may become infected after tick bite in recreational zones in suburban park areas. Currently, very few publications regarding prevalence of tick-borne pathogens in St. Petersburg area are available. In our study, questing ticks flagged in park zone (northern coast of Finnish Gulf, Kurortny District) were examined with PCR for carriage of pathogenic B. burgdorferi sensu lato complex. In addition, samples positive for Borrelia DNA signal were further genotyped with species-specific primers against rpoBgene fragment. It was found that Ixodes persulcatus dominated in this area. Prevalence of Borrelia burgdorferi s.l. complex comprised 9.33%. Genospecies B. afzelii and less frequently B. garinii were detected. A mixt-infection with two Borel-lia species was detected in one sample. Interestingly, all Borrelia-infected ticks belonged to I. persulcatus suggesting a closer association for certain species in «pathogen-vector» system. Our findings are essential in investigating distribution of ixodid borreliosis foci in St. Petersburg and suburbs, obtaining new data regarding epidemiology, diagnostics, treatment and prevention of this infection. It is noteworthy than prevalence of pathogenic Borrelia spp. vs. tick-borne encephalitis virus in vectors was higher thereby accounting for its higher morbidity. Comparing our data with those published elsewhere by European researchers allows to note that prevalence of pathogenic Borrelia spp. in ticks varies broadly in diverse geographic regions. It is necessary to take into consideration that prevalence of Borrelia markers achieves ~10% in ticks given frequent attendance of park areas near St. Petersburg that point at risk of developing bor-reliosis in recreational zones.Yu. A. PanferovaA. N. VaganovaO. A. FreylikhmanK. A. TretyakovS. G. MedvedevA. O. Shapar’N. K. TokarevichSankt-Peterburg : NIIÈM imeni Pasteraarticleixodid tick-borne borreliosisborreliagenospeciespcrprevalenceInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 10, Iss 1, Pp 175-179 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	ixodid tick-borne borreliosis borrelia genospecies pcr prevalence Infectious and parasitic diseases RC109-216
spellingShingle	ixodid tick-borne borreliosis borrelia genospecies pcr prevalence Infectious and parasitic diseases RC109-216 Yu. A. Panferova A. N. Vaganova O. A. Freylikhman K. A. Tretyakov S. G. Medvedev A. O. Shapar’ N. K. Tokarevich Prevalence of <i>Borrelia burgdorferi sensu</i> lato genetic markers in blood-sucking ticks in suburban park zones in Saint Petersburg
description	Tick-borne bacterial and viral infections are widespread in middle latitudes of the Northern hemisphere. Natural foci of such infections coincide with geographic areas inhabited by ixodid ticks. Ixodid tick-borne borreliosis is a pressing issue for some territories of Russia, especially for the North-Western Federal District and St. Petersburg megalopolis as well as adjacent areas of the Leningrad District, where people may become infected after tick bite in recreational zones in suburban park areas. Currently, very few publications regarding prevalence of tick-borne pathogens in St. Petersburg area are available. In our study, questing ticks flagged in park zone (northern coast of Finnish Gulf, Kurortny District) were examined with PCR for carriage of pathogenic B. burgdorferi sensu lato complex. In addition, samples positive for Borrelia DNA signal were further genotyped with species-specific primers against rpoBgene fragment. It was found that Ixodes persulcatus dominated in this area. Prevalence of Borrelia burgdorferi s.l. complex comprised 9.33%. Genospecies B. afzelii and less frequently B. garinii were detected. A mixt-infection with two Borel-lia species was detected in one sample. Interestingly, all Borrelia-infected ticks belonged to I. persulcatus suggesting a closer association for certain species in «pathogen-vector» system. Our findings are essential in investigating distribution of ixodid borreliosis foci in St. Petersburg and suburbs, obtaining new data regarding epidemiology, diagnostics, treatment and prevention of this infection. It is noteworthy than prevalence of pathogenic Borrelia spp. vs. tick-borne encephalitis virus in vectors was higher thereby accounting for its higher morbidity. Comparing our data with those published elsewhere by European researchers allows to note that prevalence of pathogenic Borrelia spp. in ticks varies broadly in diverse geographic regions. It is necessary to take into consideration that prevalence of Borrelia markers achieves ~10% in ticks given frequent attendance of park areas near St. Petersburg that point at risk of developing bor-reliosis in recreational zones.
format	article
author	Yu. A. Panferova A. N. Vaganova O. A. Freylikhman K. A. Tretyakov S. G. Medvedev A. O. Shapar’ N. K. Tokarevich
author_facet	Yu. A. Panferova A. N. Vaganova O. A. Freylikhman K. A. Tretyakov S. G. Medvedev A. O. Shapar’ N. K. Tokarevich
author_sort	Yu. A. Panferova
title	Prevalence of <i>Borrelia burgdorferi sensu</i> lato genetic markers in blood-sucking ticks in suburban park zones in Saint Petersburg
title_short	Prevalence of <i>Borrelia burgdorferi sensu</i> lato genetic markers in blood-sucking ticks in suburban park zones in Saint Petersburg
title_full	Prevalence of <i>Borrelia burgdorferi sensu</i> lato genetic markers in blood-sucking ticks in suburban park zones in Saint Petersburg
title_fullStr	Prevalence of <i>Borrelia burgdorferi sensu</i> lato genetic markers in blood-sucking ticks in suburban park zones in Saint Petersburg
title_full_unstemmed	Prevalence of <i>Borrelia burgdorferi sensu</i> lato genetic markers in blood-sucking ticks in suburban park zones in Saint Petersburg
title_sort	prevalence of <i>borrelia burgdorferi sensu</i> lato genetic markers in blood-sucking ticks in suburban park zones in saint petersburg
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2020
url	https://doaj.org/article/3c86cd723b3042bd92c143e5efa53155
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Prevalence of <i>Borrelia burgdorferi sensu</i> lato genetic markers in blood-sucking ticks in suburban park zones in Saint Petersburg

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