Elucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during One-Step Nucleic Acid Amplification

Abstract One-step nucleic acid amplification (OSNA) is an established method for intraoperative diagnosis of breast cancer metastasis in sentinel lymph nodes, based on quantification of CK19 mRNA, specific to breast epithelial cells. Inhibitors interfere with the PCR amplification process of PCR. Th...

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Autores principales:	Yoshiya Horimoto, Masahiko Tanabe, Saiko Kazuno, Yoshiki Miura, Kaoru Mogushi, Hiroshi Sonoue, Atsushi Arakawa, Kazunori Kajino, Toshiyuki Kobayashi, Mitsue Saito
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c8732659ff74b27a1c63e428c0fa47c
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spelling	oai:doaj.org-article:3c8732659ff74b27a1c63e428c0fa47c2021-12-02T11:40:24ZElucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during One-Step Nucleic Acid Amplification10.1038/s41598-018-25911-w2045-2322https://doaj.org/article/3c8732659ff74b27a1c63e428c0fa47c2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25911-whttps://doaj.org/toc/2045-2322Abstract One-step nucleic acid amplification (OSNA) is an established method for intraoperative diagnosis of breast cancer metastasis in sentinel lymph nodes, based on quantification of CK19 mRNA, specific to breast epithelial cells. Inhibitors interfere with the PCR amplification process of PCR. Thus, OSNA, based on gene amplification without RNA purification, might be impacted by numerous factors persisting in a sample, and thereby potentially acting as PCR inhibitors. However, neither the characteristics of breast cancers showing inhibitory effects during OSNA, nor any of the possible inhibitors, have as yet been identified. Inhibitory effects detected during OSNA in 72 metastatic lymph nodes and the patients’ clinicopathological features were examined. Left-over OSNA samples were analyzed with mass spectrometry to identify proteins possibly acting as inhibitors. Most tumors showed inhibitory effects, though to varying degrees. Large tumor, young age and high tumor-infiltrating lymphocyte counts were related to stronger inhibitory effects. Proteome analysis revealed elevations in RPB9 protein and EIF2 signaling upregulation in samples showing strong inhibitory effects. Tumors showing strong inhibitory effects had clinically relevant characteristics, including large size and extensive tumor-infiltrating lymphocyte involvement. Identifying inhibitors in OSNA might provide new insights into breast cancer biology as well as advancing the current technology.Yoshiya HorimotoMasahiko TanabeSaiko KazunoYoshiki MiuraKaoru MogushiHiroshi SonoueAtsushi ArakawaKazunori KajinoToshiyuki KobayashiMitsue SaitoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoshiya Horimoto Masahiko Tanabe Saiko Kazuno Yoshiki Miura Kaoru Mogushi Hiroshi Sonoue Atsushi Arakawa Kazunori Kajino Toshiyuki Kobayashi Mitsue Saito Elucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during One-Step Nucleic Acid Amplification
description	Abstract One-step nucleic acid amplification (OSNA) is an established method for intraoperative diagnosis of breast cancer metastasis in sentinel lymph nodes, based on quantification of CK19 mRNA, specific to breast epithelial cells. Inhibitors interfere with the PCR amplification process of PCR. Thus, OSNA, based on gene amplification without RNA purification, might be impacted by numerous factors persisting in a sample, and thereby potentially acting as PCR inhibitors. However, neither the characteristics of breast cancers showing inhibitory effects during OSNA, nor any of the possible inhibitors, have as yet been identified. Inhibitory effects detected during OSNA in 72 metastatic lymph nodes and the patients’ clinicopathological features were examined. Left-over OSNA samples were analyzed with mass spectrometry to identify proteins possibly acting as inhibitors. Most tumors showed inhibitory effects, though to varying degrees. Large tumor, young age and high tumor-infiltrating lymphocyte counts were related to stronger inhibitory effects. Proteome analysis revealed elevations in RPB9 protein and EIF2 signaling upregulation in samples showing strong inhibitory effects. Tumors showing strong inhibitory effects had clinically relevant characteristics, including large size and extensive tumor-infiltrating lymphocyte involvement. Identifying inhibitors in OSNA might provide new insights into breast cancer biology as well as advancing the current technology.
format	article
author	Yoshiya Horimoto Masahiko Tanabe Saiko Kazuno Yoshiki Miura Kaoru Mogushi Hiroshi Sonoue Atsushi Arakawa Kazunori Kajino Toshiyuki Kobayashi Mitsue Saito
author_facet	Yoshiya Horimoto Masahiko Tanabe Saiko Kazuno Yoshiki Miura Kaoru Mogushi Hiroshi Sonoue Atsushi Arakawa Kazunori Kajino Toshiyuki Kobayashi Mitsue Saito
author_sort	Yoshiya Horimoto
title	Elucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during One-Step Nucleic Acid Amplification
title_short	Elucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during One-Step Nucleic Acid Amplification
title_full	Elucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during One-Step Nucleic Acid Amplification
title_fullStr	Elucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during One-Step Nucleic Acid Amplification
title_full_unstemmed	Elucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during One-Step Nucleic Acid Amplification
title_sort	elucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during one-step nucleic acid amplification
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/3c8732659ff74b27a1c63e428c0fa47c
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Elucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during One-Step Nucleic Acid Amplification

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