Simplified modeling of E. coli mortality after genome damage induced by UV-C light exposure

Abstract UV light is a group of high-energy waves from the electromagnetic spectrum. There are three types of UV radiations: UV-A, -B and -C. UV-C light are the highest in energy, but most are retained by the ozone layer. UV-A and -B reach the earth’s surface and cause damage on living organisms, be...

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Autores principales:	Jaime Sánchez-Navarrete, Nancy Jannet Ruiz-Pérez, Armando Guerra-Trejo, Julia Dolores Toscano-Garibay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c88b84ff8ac465b9a6aeb3f5d83206a
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spelling	oai:doaj.org-article:3c88b84ff8ac465b9a6aeb3f5d83206a2021-12-02T15:39:49ZSimplified modeling of E. coli mortality after genome damage induced by UV-C light exposure10.1038/s41598-020-67838-12045-2322https://doaj.org/article/3c88b84ff8ac465b9a6aeb3f5d83206a2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-67838-1https://doaj.org/toc/2045-2322Abstract UV light is a group of high-energy waves from the electromagnetic spectrum. There are three types of UV radiations: UV-A, -B and -C. UV-C light are the highest in energy, but most are retained by the ozone layer. UV-A and -B reach the earth’s surface and cause damage on living organisms, being considered as mutagenic physical agents. Numerous test models are used to study UV mutagenicity; some include special lamps, cell cultures and mathematical modeling. Mercury lamps are affordable and useful sources of UV-C light due to their emission at near the maximum absorption peak of nucleic acids. E. coli cultures are widely used because they have DNA-damage and -repairing mechanisms fairly similar to humans. In here we present two simple models that describe UV-C light incidence on a genome matrix, using fundamental quantum–mechanical concepts and considering light as a particle with a discontinuous distribution. To test the accuracy of our equations, stationary phase cultures of several E. coli strains were exposed to UV-C light in 30 s-intervals. Surviving CFUs were counted and survival/mortality curves were constructed. These graphs adjusted with high goodness of fit to the regression predictions. Results were also analyzed using three main parameters: quantum yield, specific speed and time of mortality.Jaime Sánchez-NavarreteNancy Jannet Ruiz-PérezArmando Guerra-TrejoJulia Dolores Toscano-GaribayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jaime Sánchez-Navarrete Nancy Jannet Ruiz-Pérez Armando Guerra-Trejo Julia Dolores Toscano-Garibay Simplified modeling of E. coli mortality after genome damage induced by UV-C light exposure
description	Abstract UV light is a group of high-energy waves from the electromagnetic spectrum. There are three types of UV radiations: UV-A, -B and -C. UV-C light are the highest in energy, but most are retained by the ozone layer. UV-A and -B reach the earth’s surface and cause damage on living organisms, being considered as mutagenic physical agents. Numerous test models are used to study UV mutagenicity; some include special lamps, cell cultures and mathematical modeling. Mercury lamps are affordable and useful sources of UV-C light due to their emission at near the maximum absorption peak of nucleic acids. E. coli cultures are widely used because they have DNA-damage and -repairing mechanisms fairly similar to humans. In here we present two simple models that describe UV-C light incidence on a genome matrix, using fundamental quantum–mechanical concepts and considering light as a particle with a discontinuous distribution. To test the accuracy of our equations, stationary phase cultures of several E. coli strains were exposed to UV-C light in 30 s-intervals. Surviving CFUs were counted and survival/mortality curves were constructed. These graphs adjusted with high goodness of fit to the regression predictions. Results were also analyzed using three main parameters: quantum yield, specific speed and time of mortality.
format	article
author	Jaime Sánchez-Navarrete Nancy Jannet Ruiz-Pérez Armando Guerra-Trejo Julia Dolores Toscano-Garibay
author_facet	Jaime Sánchez-Navarrete Nancy Jannet Ruiz-Pérez Armando Guerra-Trejo Julia Dolores Toscano-Garibay
author_sort	Jaime Sánchez-Navarrete
title	Simplified modeling of E. coli mortality after genome damage induced by UV-C light exposure
title_short	Simplified modeling of E. coli mortality after genome damage induced by UV-C light exposure
title_full	Simplified modeling of E. coli mortality after genome damage induced by UV-C light exposure
title_fullStr	Simplified modeling of E. coli mortality after genome damage induced by UV-C light exposure
title_full_unstemmed	Simplified modeling of E. coli mortality after genome damage induced by UV-C light exposure
title_sort	simplified modeling of e. coli mortality after genome damage induced by uv-c light exposure
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/3c88b84ff8ac465b9a6aeb3f5d83206a
work_keys_str_mv	AT jaimesancheznavarrete simplifiedmodelingofecolimortalityaftergenomedamageinducedbyuvclightexposure AT nancyjannetruizperez simplifiedmodelingofecolimortalityaftergenomedamageinducedbyuvclightexposure AT armandoguerratrejo simplifiedmodelingofecolimortalityaftergenomedamageinducedbyuvclightexposure AT juliadolorestoscanogaribay simplifiedmodelingofecolimortalityaftergenomedamageinducedbyuvclightexposure
_version_	1718385866948214784

Simplified modeling of E. coli mortality after genome damage induced by UV-C light exposure

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