Alterations in the Helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.

Jasmonate inducible plant leucine aminopeptidase (LAP) is proposed to serve as direct defense in the insect midgut. However, exact functions of inducible plant LAPs in the insect midgut remain to be estimated. In the present investigation, we report the direct defensive role of pigeon pea inducible...

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Autores principales:	Purushottam R Lomate, Bhakti R Jadhav, Ashok P Giri, Vandana K Hivrale
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c8e40df5989493597cb9996913ffe63
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spelling	oai:doaj.org-article:3c8e40df5989493597cb9996913ffe632021-11-18T08:53:08ZAlterations in the Helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.1932-620310.1371/journal.pone.0074889https://doaj.org/article/3c8e40df5989493597cb9996913ffe632013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24098675/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Jasmonate inducible plant leucine aminopeptidase (LAP) is proposed to serve as direct defense in the insect midgut. However, exact functions of inducible plant LAPs in the insect midgut remain to be estimated. In the present investigation, we report the direct defensive role of pigeon pea inducible LAP in the midgut of Helicoverpa armigera (Lepidoptera: Noctuidae) and responses of midgut soluble aminopeptidases and serine proteinases upon LAP ingestion. Larval growth and survival was significantly reduced on the diets supplemented with pigeon pea LAP. Aminopeptidase activities in larvae remain unaltered in presence or absence of inducible LAP in the diet. On the contrary, serine proteinase activities were significantly decreased in the larvae reared on pigeon pea LAP containing diet as compared to larvae fed on diet without LAP. Our data suggest that pigeon pea inducible LAP is responsible for the degradation of midgut serine proteinases upon ingestion. Reduction in the aminopeptidase activity with LpNA in the H. armigera larvae was compensated with an induction of aminopeptidase activity with ApNA. Our findings could be helpful to further dissect the roles of plant inducible LAPs in the direct plant defense against herbivory.Purushottam R LomateBhakti R JadhavAshok P GiriVandana K HivralePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e74889 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Purushottam R Lomate Bhakti R Jadhav Ashok P Giri Vandana K Hivrale Alterations in the Helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.
description	Jasmonate inducible plant leucine aminopeptidase (LAP) is proposed to serve as direct defense in the insect midgut. However, exact functions of inducible plant LAPs in the insect midgut remain to be estimated. In the present investigation, we report the direct defensive role of pigeon pea inducible LAP in the midgut of Helicoverpa armigera (Lepidoptera: Noctuidae) and responses of midgut soluble aminopeptidases and serine proteinases upon LAP ingestion. Larval growth and survival was significantly reduced on the diets supplemented with pigeon pea LAP. Aminopeptidase activities in larvae remain unaltered in presence or absence of inducible LAP in the diet. On the contrary, serine proteinase activities were significantly decreased in the larvae reared on pigeon pea LAP containing diet as compared to larvae fed on diet without LAP. Our data suggest that pigeon pea inducible LAP is responsible for the degradation of midgut serine proteinases upon ingestion. Reduction in the aminopeptidase activity with LpNA in the H. armigera larvae was compensated with an induction of aminopeptidase activity with ApNA. Our findings could be helpful to further dissect the roles of plant inducible LAPs in the direct plant defense against herbivory.
format	article
author	Purushottam R Lomate Bhakti R Jadhav Ashok P Giri Vandana K Hivrale
author_facet	Purushottam R Lomate Bhakti R Jadhav Ashok P Giri Vandana K Hivrale
author_sort	Purushottam R Lomate
title	Alterations in the Helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.
title_short	Alterations in the Helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.
title_full	Alterations in the Helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.
title_fullStr	Alterations in the Helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.
title_full_unstemmed	Alterations in the Helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.
title_sort	alterations in the helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3c8e40df5989493597cb9996913ffe63
work_keys_str_mv	AT purushottamrlomate alterationsinthehelicoverpaarmigeramidgutdigestivephysiologyafteringestionofpigeonpeainducibleleucineaminopeptidase AT bhaktirjadhav alterationsinthehelicoverpaarmigeramidgutdigestivephysiologyafteringestionofpigeonpeainducibleleucineaminopeptidase AT ashokpgiri alterationsinthehelicoverpaarmigeramidgutdigestivephysiologyafteringestionofpigeonpeainducibleleucineaminopeptidase AT vandanakhivrale alterationsinthehelicoverpaarmigeramidgutdigestivephysiologyafteringestionofpigeonpeainducibleleucineaminopeptidase
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Alterations in the Helicoverpa armigera midgut digestive physiology after ingestion of pigeon pea inducible leucine aminopeptidase.

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