Biodistribution, Clearance And Morphological Alterations Of Intravenously Administered Iron Oxide Nanoparticles In Male Wistar Rats

Usha Singh Gaharwar, Ramovatar Meena, Paulraj Rajamani School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaCorrespondence: Paulraj RajamaniSchool of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaTel +91 11 2670 4162Email paulrajr@hotmai...

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Autores principales:	Gaharwar US, Meena R, Rajamani P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	metal oxide nanoparticles bioaccumulation ultrastructural changes toxicity metabolic cages Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3c91f674251c423da997e10ffab8d3f4
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spelling	oai:doaj.org-article:3c91f674251c423da997e10ffab8d3f42021-12-02T09:45:45ZBiodistribution, Clearance And Morphological Alterations Of Intravenously Administered Iron Oxide Nanoparticles In Male Wistar Rats1178-2013https://doaj.org/article/3c91f674251c423da997e10ffab8d3f42019-12-01T00:00:00Zhttps://www.dovepress.com/biodistribution-clearance-and-morphological-alterations-of-intravenous-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Usha Singh Gaharwar, Ramovatar Meena, Paulraj Rajamani School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaCorrespondence: Paulraj RajamaniSchool of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaTel +91 11 2670 4162Email paulrajr@hotmail.comIntroduction: Nanoparticles are used worldwide because of their unique properties, with large-scale application in various fields, such as medicine, cosmetics and industries. In view of their widespread use, the potential adverse effects of nanoparticles have become a significant cause for concern, in terms of not only human health and safety but also the environment. The present investigation focused on establishing the bioaccumulation patterns and ultrastructural changes induced by retained iron oxide nanoparticles (IONPs) in various target organs of rats.Methods: Twenty-four male Wistar rats were randomly divided into four groups. Experimental animals were intravenously administered different doses of IONPs (7.5 mg/kg, 15 mg/kg and 30 mg/kg) once in a week for 4 weeks. Urine and feces samples were collected on a daily basis to assess nanoparticle clearance and analyzed via atomic absorption spectroscopy (AAS). At the end of the experiment, rats were euthanized and different organs, including spleen, liver, kidney, lung, heart, testis and brain, were dissected. Bioaccumulation of iron in organs and ultrastructural changes induced by IONPs were determined.Results: The maximal concentration of iron was detected in spleen and minimal concentration in the brain. The level of iron accumulation in organs was as follows: spleen>blood>liver>kidney>lung>heart>testis>brain. The excretion profile in urine revealed maximum excretion on the day following administration that was maintained until day 28, whereas the iron content in feces remained high during the first three days after injection. A similar pattern was observed throughout the duration of the experiment. Ultrastructural alterations were detected in spleen, kidney, lung, heart, testis, brain and liver, indicative of cellular damage induced by accumulating nanoparticles in these organs.Conclusion: Intravenous administration of IONPs results in ultrastructural changes and dose-dependent bioaccumulation in different organs of rats.Keywords: metal oxide nanoparticles, bioaccumulation, ultrastructural changes, toxicity, metabolic cagesGaharwar USMeena RRajamani PDove Medical Pressarticlemetal oxide nanoparticlesbioaccumulationultrastructural changestoxicitymetabolic cagesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9677-9692 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	metal oxide nanoparticles bioaccumulation ultrastructural changes toxicity metabolic cages Medicine (General) R5-920
spellingShingle	metal oxide nanoparticles bioaccumulation ultrastructural changes toxicity metabolic cages Medicine (General) R5-920 Gaharwar US Meena R Rajamani P Biodistribution, Clearance And Morphological Alterations Of Intravenously Administered Iron Oxide Nanoparticles In Male Wistar Rats
description	Usha Singh Gaharwar, Ramovatar Meena, Paulraj Rajamani School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaCorrespondence: Paulraj RajamaniSchool of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaTel +91 11 2670 4162Email paulrajr@hotmail.comIntroduction: Nanoparticles are used worldwide because of their unique properties, with large-scale application in various fields, such as medicine, cosmetics and industries. In view of their widespread use, the potential adverse effects of nanoparticles have become a significant cause for concern, in terms of not only human health and safety but also the environment. The present investigation focused on establishing the bioaccumulation patterns and ultrastructural changes induced by retained iron oxide nanoparticles (IONPs) in various target organs of rats.Methods: Twenty-four male Wistar rats were randomly divided into four groups. Experimental animals were intravenously administered different doses of IONPs (7.5 mg/kg, 15 mg/kg and 30 mg/kg) once in a week for 4 weeks. Urine and feces samples were collected on a daily basis to assess nanoparticle clearance and analyzed via atomic absorption spectroscopy (AAS). At the end of the experiment, rats were euthanized and different organs, including spleen, liver, kidney, lung, heart, testis and brain, were dissected. Bioaccumulation of iron in organs and ultrastructural changes induced by IONPs were determined.Results: The maximal concentration of iron was detected in spleen and minimal concentration in the brain. The level of iron accumulation in organs was as follows: spleen>blood>liver>kidney>lung>heart>testis>brain. The excretion profile in urine revealed maximum excretion on the day following administration that was maintained until day 28, whereas the iron content in feces remained high during the first three days after injection. A similar pattern was observed throughout the duration of the experiment. Ultrastructural alterations were detected in spleen, kidney, lung, heart, testis, brain and liver, indicative of cellular damage induced by accumulating nanoparticles in these organs.Conclusion: Intravenous administration of IONPs results in ultrastructural changes and dose-dependent bioaccumulation in different organs of rats.Keywords: metal oxide nanoparticles, bioaccumulation, ultrastructural changes, toxicity, metabolic cages
format	article
author	Gaharwar US Meena R Rajamani P
author_facet	Gaharwar US Meena R Rajamani P
author_sort	Gaharwar US
title	Biodistribution, Clearance And Morphological Alterations Of Intravenously Administered Iron Oxide Nanoparticles In Male Wistar Rats
title_short	Biodistribution, Clearance And Morphological Alterations Of Intravenously Administered Iron Oxide Nanoparticles In Male Wistar Rats
title_full	Biodistribution, Clearance And Morphological Alterations Of Intravenously Administered Iron Oxide Nanoparticles In Male Wistar Rats
title_fullStr	Biodistribution, Clearance And Morphological Alterations Of Intravenously Administered Iron Oxide Nanoparticles In Male Wistar Rats
title_full_unstemmed	Biodistribution, Clearance And Morphological Alterations Of Intravenously Administered Iron Oxide Nanoparticles In Male Wistar Rats
title_sort	biodistribution, clearance and morphological alterations of intravenously administered iron oxide nanoparticles in male wistar rats
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/3c91f674251c423da997e10ffab8d3f4
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Biodistribution, Clearance And Morphological Alterations Of Intravenously Administered Iron Oxide Nanoparticles In Male Wistar Rats

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