Curcumin nanoparticles ameliorate ICAM-1 expression in TNF-α-treated lung epithelial cells through p47 (phox) and MAPKs/AP-1 pathways.
Upregulation of intercellular adhesion molecule-1 (ICAM-1) involves adhesions between both circulating and resident leukocytes and the human lung epithelial cells during lung inflammatory reactions. We have previously demonstrated that curcumin-loaded polyvinylpyrrolidone nanoparticles (CURN) improv...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/3c93774338314fa599540cb04625c851 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:3c93774338314fa599540cb04625c851 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:3c93774338314fa599540cb04625c8512021-11-18T07:46:10ZCurcumin nanoparticles ameliorate ICAM-1 expression in TNF-α-treated lung epithelial cells through p47 (phox) and MAPKs/AP-1 pathways.1932-620310.1371/journal.pone.0063845https://doaj.org/article/3c93774338314fa599540cb04625c8512013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23671702/?tool=EBIhttps://doaj.org/toc/1932-6203Upregulation of intercellular adhesion molecule-1 (ICAM-1) involves adhesions between both circulating and resident leukocytes and the human lung epithelial cells during lung inflammatory reactions. We have previously demonstrated that curcumin-loaded polyvinylpyrrolidone nanoparticles (CURN) improve the anti-inflammatory and anti-oxidative properties of curcumin in hepatocytes. In this study, we focused on the effects of CURN on the expression of ICAM-1 in TNF-α-treated lung epithelial cells and compared these to the effects of curcumin water preparation (CURH). TNF-αinduced ICAM-1 expression, ROS production, and cell-cell adhesion were significantly attenuated by the pretreatment with antioxidants (DPI, APO, or NAC) and CURN, but not by CURH, as revealed by western blot analysis, RT-PCR, promoter assay, and ROS detection and adhesion assay. In addition, treatment of TNF-α-treated cells with CURN and antioxidants also resulted in an inhibition of activation of p47 (phox) and phosphorylation of MAPKs, as compared to that using CURH. Our findings also suggest that phosphorylation of MAPKs may eventually lead to the activation of transcription factors. We also observed that the effects of TNF-α treatment for 30 min, which includes a significant increase in the binding activity of AP-1 and phosphorylation of c-jun and c-fos genes, were reduced by CURN treatment. In vivo studies have revealed that CURN improved the anti-inflammation activities of CURH in the lung epithelial cells of TNF-α-treated mice. Our results indicate that curcumin-loaded polyvinylpyrrolidone nanoparticles may potentially serve as an anti-inflammatory drug for the treatment of respiratory diseases.Feng-Lin YenMing-Horng TsaiChuen-Mao YangChan-Jung LiangChun-Ching LinYao-Chang ChiangHui-Chun LeeHorng-Huey KoChiang-Wen LeePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63845 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Feng-Lin Yen Ming-Horng Tsai Chuen-Mao Yang Chan-Jung Liang Chun-Ching Lin Yao-Chang Chiang Hui-Chun Lee Horng-Huey Ko Chiang-Wen Lee Curcumin nanoparticles ameliorate ICAM-1 expression in TNF-α-treated lung epithelial cells through p47 (phox) and MAPKs/AP-1 pathways. |
| description |
Upregulation of intercellular adhesion molecule-1 (ICAM-1) involves adhesions between both circulating and resident leukocytes and the human lung epithelial cells during lung inflammatory reactions. We have previously demonstrated that curcumin-loaded polyvinylpyrrolidone nanoparticles (CURN) improve the anti-inflammatory and anti-oxidative properties of curcumin in hepatocytes. In this study, we focused on the effects of CURN on the expression of ICAM-1 in TNF-α-treated lung epithelial cells and compared these to the effects of curcumin water preparation (CURH). TNF-αinduced ICAM-1 expression, ROS production, and cell-cell adhesion were significantly attenuated by the pretreatment with antioxidants (DPI, APO, or NAC) and CURN, but not by CURH, as revealed by western blot analysis, RT-PCR, promoter assay, and ROS detection and adhesion assay. In addition, treatment of TNF-α-treated cells with CURN and antioxidants also resulted in an inhibition of activation of p47 (phox) and phosphorylation of MAPKs, as compared to that using CURH. Our findings also suggest that phosphorylation of MAPKs may eventually lead to the activation of transcription factors. We also observed that the effects of TNF-α treatment for 30 min, which includes a significant increase in the binding activity of AP-1 and phosphorylation of c-jun and c-fos genes, were reduced by CURN treatment. In vivo studies have revealed that CURN improved the anti-inflammation activities of CURH in the lung epithelial cells of TNF-α-treated mice. Our results indicate that curcumin-loaded polyvinylpyrrolidone nanoparticles may potentially serve as an anti-inflammatory drug for the treatment of respiratory diseases. |
| format |
article |
| author |
Feng-Lin Yen Ming-Horng Tsai Chuen-Mao Yang Chan-Jung Liang Chun-Ching Lin Yao-Chang Chiang Hui-Chun Lee Horng-Huey Ko Chiang-Wen Lee |
| author_facet |
Feng-Lin Yen Ming-Horng Tsai Chuen-Mao Yang Chan-Jung Liang Chun-Ching Lin Yao-Chang Chiang Hui-Chun Lee Horng-Huey Ko Chiang-Wen Lee |
| author_sort |
Feng-Lin Yen |
| title |
Curcumin nanoparticles ameliorate ICAM-1 expression in TNF-α-treated lung epithelial cells through p47 (phox) and MAPKs/AP-1 pathways. |
| title_short |
Curcumin nanoparticles ameliorate ICAM-1 expression in TNF-α-treated lung epithelial cells through p47 (phox) and MAPKs/AP-1 pathways. |
| title_full |
Curcumin nanoparticles ameliorate ICAM-1 expression in TNF-α-treated lung epithelial cells through p47 (phox) and MAPKs/AP-1 pathways. |
| title_fullStr |
Curcumin nanoparticles ameliorate ICAM-1 expression in TNF-α-treated lung epithelial cells through p47 (phox) and MAPKs/AP-1 pathways. |
| title_full_unstemmed |
Curcumin nanoparticles ameliorate ICAM-1 expression in TNF-α-treated lung epithelial cells through p47 (phox) and MAPKs/AP-1 pathways. |
| title_sort |
curcumin nanoparticles ameliorate icam-1 expression in tnf-α-treated lung epithelial cells through p47 (phox) and mapks/ap-1 pathways. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/3c93774338314fa599540cb04625c851 |
| work_keys_str_mv |
AT fenglinyen curcuminnanoparticlesameliorateicam1expressionintnfatreatedlungepithelialcellsthroughp47phoxandmapksap1pathways AT minghorngtsai curcuminnanoparticlesameliorateicam1expressionintnfatreatedlungepithelialcellsthroughp47phoxandmapksap1pathways AT chuenmaoyang curcuminnanoparticlesameliorateicam1expressionintnfatreatedlungepithelialcellsthroughp47phoxandmapksap1pathways AT chanjungliang curcuminnanoparticlesameliorateicam1expressionintnfatreatedlungepithelialcellsthroughp47phoxandmapksap1pathways AT chunchinglin curcuminnanoparticlesameliorateicam1expressionintnfatreatedlungepithelialcellsthroughp47phoxandmapksap1pathways AT yaochangchiang curcuminnanoparticlesameliorateicam1expressionintnfatreatedlungepithelialcellsthroughp47phoxandmapksap1pathways AT huichunlee curcuminnanoparticlesameliorateicam1expressionintnfatreatedlungepithelialcellsthroughp47phoxandmapksap1pathways AT hornghueyko curcuminnanoparticlesameliorateicam1expressionintnfatreatedlungepithelialcellsthroughp47phoxandmapksap1pathways AT chiangwenlee curcuminnanoparticlesameliorateicam1expressionintnfatreatedlungepithelialcellsthroughp47phoxandmapksap1pathways |
| _version_ |
1718422963458408448 |