Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines

Weizhong Zhao1, Xun Lu3, Yuan Yuan1, Changsheng Liu1, Baican Yang3, Hua Hong1, Guoying Wang3, Fanyan Zeng21The State Key Laboratory of Bioreactor Engineering, 2Key Laboratory for Ultrafine Materials of Ministry of Education and Engineering Research Center for Biomedical Materials of Ministry of Educ...

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Autores principales:	Zhao W, Lu X, Yuan Y, Liu C, Yang B, Hong H, Wang G, Zeng F
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3c956742fe5f4e3583c76843f0787ce3
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spelling	oai:doaj.org-article:3c956742fe5f4e3583c76843f0787ce32021-12-02T02:34:13ZEffect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines1176-91141178-2013https://doaj.org/article/3c956742fe5f4e3583c76843f0787ce32011-08-01T00:00:00Zhttp://www.dovepress.com/effect-of-size-and-processing-method-on-the-cytotoxicity-of-realgar-na-a8001https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Weizhong Zhao1, Xun Lu3, Yuan Yuan1, Changsheng Liu1, Baican Yang3, Hua Hong1, Guoying Wang3, Fanyan Zeng21The State Key Laboratory of Bioreactor Engineering, 2Key Laboratory for Ultrafine Materials of Ministry of Education and Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, 3Pharmacy Department of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People&rsquo;s Republic of ChinaAbstract: In this study, the effects of the size and Chinese traditional processing (including elutriation, water cleaning, acid cleaning, alkali cleaning) on realgar nanoparticles (RN)-induced antitumor activity in human osteosarcoma cell lines (MG-63) and hepatoma carcinoma cell lines (HepG-2) were investigated. The human normal liver cell line (L-02) was used as control. RN was prepared by high-energy ball milling technology. The results showed that with the assistance of sodium dodecyl sulfate, the size of realgar could be reduced to 127 nm after 12 hours&rsquo; ball milling. The surface charge was decreased from 0.83 eV to &minus;17.85 eV and the content of As2O3 clearly increased. Except for elutriation, the processing methods did not clearly change the size of the RN, but the content of As2O3 was reduced dramatically. In vitro MTT tests indicated that in the two cancer cell lines, RN cytotoxicity was more intense than that of the coarse realgar nanoparticles, and cytotoxicity was typically time- and concentration-dependent. Also, RN cytotoxicities in the HepG-2 and L-02 cells all increased with increasing milling time. Due to the reduction of the As2O3 content, water cleaning, acid cleaning, and alkali cleaning decreased RN cytotoxicity in HepG-2, but RN after elutriation, with the lowest As2O3 (3.5 mg/g) and the smallest size (109.3 nm), showed comparable cytotoxicity in HepG-2 to RN without treatment. Meanwhile, RN-induced cytotoxicity in L-02 cells was clearly reduced. Therefore, it can be concluded that RN may provide a strong antiproliferation effect in the MG-63 and HepG-2 cells. Elutriation processing is a suitable approach to limit the dangerous side-effects of As2O3, while maintaining the effectiveness of RN.Keywords: realgar nanoparticles, cytotoxicity, size, processing, apoptosisZhao WLu XYuan YLiu CYang BHong HWang GZeng FDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1569-1577 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhao W Lu X Yuan Y Liu C Yang B Hong H Wang G Zeng F Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
description	Weizhong Zhao1, Xun Lu3, Yuan Yuan1, Changsheng Liu1, Baican Yang3, Hua Hong1, Guoying Wang3, Fanyan Zeng21The State Key Laboratory of Bioreactor Engineering, 2Key Laboratory for Ultrafine Materials of Ministry of Education and Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, 3Pharmacy Department of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People&rsquo;s Republic of ChinaAbstract: In this study, the effects of the size and Chinese traditional processing (including elutriation, water cleaning, acid cleaning, alkali cleaning) on realgar nanoparticles (RN)-induced antitumor activity in human osteosarcoma cell lines (MG-63) and hepatoma carcinoma cell lines (HepG-2) were investigated. The human normal liver cell line (L-02) was used as control. RN was prepared by high-energy ball milling technology. The results showed that with the assistance of sodium dodecyl sulfate, the size of realgar could be reduced to 127 nm after 12 hours&rsquo; ball milling. The surface charge was decreased from 0.83 eV to &minus;17.85 eV and the content of As2O3 clearly increased. Except for elutriation, the processing methods did not clearly change the size of the RN, but the content of As2O3 was reduced dramatically. In vitro MTT tests indicated that in the two cancer cell lines, RN cytotoxicity was more intense than that of the coarse realgar nanoparticles, and cytotoxicity was typically time- and concentration-dependent. Also, RN cytotoxicities in the HepG-2 and L-02 cells all increased with increasing milling time. Due to the reduction of the As2O3 content, water cleaning, acid cleaning, and alkali cleaning decreased RN cytotoxicity in HepG-2, but RN after elutriation, with the lowest As2O3 (3.5 mg/g) and the smallest size (109.3 nm), showed comparable cytotoxicity in HepG-2 to RN without treatment. Meanwhile, RN-induced cytotoxicity in L-02 cells was clearly reduced. Therefore, it can be concluded that RN may provide a strong antiproliferation effect in the MG-63 and HepG-2 cells. Elutriation processing is a suitable approach to limit the dangerous side-effects of As2O3, while maintaining the effectiveness of RN.Keywords: realgar nanoparticles, cytotoxicity, size, processing, apoptosis
format	article
author	Zhao W Lu X Yuan Y Liu C Yang B Hong H Wang G Zeng F
author_facet	Zhao W Lu X Yuan Y Liu C Yang B Hong H Wang G Zeng F
author_sort	Zhao W
title	Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title_short	Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title_full	Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title_fullStr	Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title_full_unstemmed	Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
title_sort	effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/3c956742fe5f4e3583c76843f0787ce3
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_version_	1718402344021917696

Effect of size and processing method on the cytotoxicity of realgar nanoparticles in cancer cell lines

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