Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours

Abstract Spontaneously occurring canine mammary tumours (CMTs) are the most common neoplasms of female unspayed dogs and are of potential importance as models for human breast cancer as well. Mortality rates are thrice higher in dogs as compared to humans with breast cancer, which can partly be attr...

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Autores principales:	Shahid Hussain, Sonal Saxena, Sameer Shrivastava, Richa Arora, Rajkumar James Singh, Subas Chandra Jena, Naveen Kumar, Anil Kumar Sharma, Monalisa Sahoo, Ashok Kumar Tiwari, Bishnu Prasad Mishra, Raj Kumar Singh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Canine Mammary Tumors (CMT) Manganese Superoxide Dismutase (MNSOD) Halo Tag Phosphoglycerate Mutase (PGAM1) Human Breast Cancer Studies Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3c95f4a99c364737afb76162ca548e7e
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spelling	oai:doaj.org-article:3c95f4a99c364737afb76162ca548e7e2021-12-02T15:08:48ZMultiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours10.1038/s41598-018-34097-02045-2322https://doaj.org/article/3c95f4a99c364737afb76162ca548e7e2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34097-0https://doaj.org/toc/2045-2322Abstract Spontaneously occurring canine mammary tumours (CMTs) are the most common neoplasms of female unspayed dogs and are of potential importance as models for human breast cancer as well. Mortality rates are thrice higher in dogs as compared to humans with breast cancer, which can partly be attributed to lack of diagnostic techniques for their early detection. Human breast cancer studies reveal role of autoantibodies in early cancer diagnosis and also the usefulness of autoantibody panels in increasing the sensitivity, as well as, specificity of diagnostic assays. Therefore, in this study, we took advantage of high-throughput Luminex technique for developing a multiplex assay to detect autoantibody signatures against 5 canine mammary tumour-associated autoantigens (TAAs). These TAAs were expressed separately as fusion proteins with halo tag at the N-terminus, which allows easy and specific covalent coupling with magnetic microspheres. The multiplex assay, comprising a panel of candidate autoantigens (TPI, PGAM1, MNSOD, CMYC & MUC1) was used for screening circulating autoantibodies in 125 dog sera samples, including 75 mammary tumour sera and 50 healthy dog sera. The area under curve (AUC) of the combined panel of biomarkers is 0.931 (p < 0.0001), which validates the discriminative potential of the panel in differentiating tumour patients from healthy controls. The assay could be conducted in 3hrs using only 1ul of serum sample and could detect clinical cases of canine mammary tumour with sensitivity and specificity of 78.6% and 90%, respectively. In this study, we report for the first time a multiplexed assay for detection of autoantibodies in canine tumours, utilizing luminex technology and halo-tag coupling strategy. Further to the best of our knowledge, autoantibodies to CMYC and MUC1 have been reported for the first time in canines in this study.Shahid HussainSonal SaxenaSameer ShrivastavaRicha AroraRajkumar James SinghSubas Chandra JenaNaveen KumarAnil Kumar SharmaMonalisa SahooAshok Kumar TiwariBishnu Prasad MishraRaj Kumar SinghNature PortfolioarticleCanine Mammary Tumors (CMT)Manganese Superoxide Dismutase (MNSOD)Halo TagPhosphoglycerate Mutase (PGAM1)Human Breast Cancer StudiesMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Canine Mammary Tumors (CMT) Manganese Superoxide Dismutase (MNSOD) Halo Tag Phosphoglycerate Mutase (PGAM1) Human Breast Cancer Studies Medicine R Science Q
spellingShingle	Canine Mammary Tumors (CMT) Manganese Superoxide Dismutase (MNSOD) Halo Tag Phosphoglycerate Mutase (PGAM1) Human Breast Cancer Studies Medicine R Science Q Shahid Hussain Sonal Saxena Sameer Shrivastava Richa Arora Rajkumar James Singh Subas Chandra Jena Naveen Kumar Anil Kumar Sharma Monalisa Sahoo Ashok Kumar Tiwari Bishnu Prasad Mishra Raj Kumar Singh Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours
description	Abstract Spontaneously occurring canine mammary tumours (CMTs) are the most common neoplasms of female unspayed dogs and are of potential importance as models for human breast cancer as well. Mortality rates are thrice higher in dogs as compared to humans with breast cancer, which can partly be attributed to lack of diagnostic techniques for their early detection. Human breast cancer studies reveal role of autoantibodies in early cancer diagnosis and also the usefulness of autoantibody panels in increasing the sensitivity, as well as, specificity of diagnostic assays. Therefore, in this study, we took advantage of high-throughput Luminex technique for developing a multiplex assay to detect autoantibody signatures against 5 canine mammary tumour-associated autoantigens (TAAs). These TAAs were expressed separately as fusion proteins with halo tag at the N-terminus, which allows easy and specific covalent coupling with magnetic microspheres. The multiplex assay, comprising a panel of candidate autoantigens (TPI, PGAM1, MNSOD, CMYC & MUC1) was used for screening circulating autoantibodies in 125 dog sera samples, including 75 mammary tumour sera and 50 healthy dog sera. The area under curve (AUC) of the combined panel of biomarkers is 0.931 (p < 0.0001), which validates the discriminative potential of the panel in differentiating tumour patients from healthy controls. The assay could be conducted in 3hrs using only 1ul of serum sample and could detect clinical cases of canine mammary tumour with sensitivity and specificity of 78.6% and 90%, respectively. In this study, we report for the first time a multiplexed assay for detection of autoantibodies in canine tumours, utilizing luminex technology and halo-tag coupling strategy. Further to the best of our knowledge, autoantibodies to CMYC and MUC1 have been reported for the first time in canines in this study.
format	article
author	Shahid Hussain Sonal Saxena Sameer Shrivastava Richa Arora Rajkumar James Singh Subas Chandra Jena Naveen Kumar Anil Kumar Sharma Monalisa Sahoo Ashok Kumar Tiwari Bishnu Prasad Mishra Raj Kumar Singh
author_facet	Shahid Hussain Sonal Saxena Sameer Shrivastava Richa Arora Rajkumar James Singh Subas Chandra Jena Naveen Kumar Anil Kumar Sharma Monalisa Sahoo Ashok Kumar Tiwari Bishnu Prasad Mishra Raj Kumar Singh
author_sort	Shahid Hussain
title	Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours
title_short	Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours
title_full	Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours
title_fullStr	Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours
title_full_unstemmed	Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours
title_sort	multiplexed autoantibody signature for serological detection of canine mammary tumours
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/3c95f4a99c364737afb76162ca548e7e
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Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours

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