Development of a yeast cell surface display method using the SpyTag/SpyCatcher system

Abstract Yeast cell surface display (YSD) has been used to engineer various proteins, including antibodies. Directed evolution, which subjects a gene to iterative rounds of mutagenesis, selection and amplification, is useful for protein engineering. In vivo continuous mutagenesis, which continuously...

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Autores principales: Kaho Kajiwara, Wataru Aoki, Naoki Koike, Mitsuyoshi Ueda
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/3c96bce5a6a04f1d89df7768632d6aad
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