Development of a yeast cell surface display method using the SpyTag/SpyCatcher system
Abstract Yeast cell surface display (YSD) has been used to engineer various proteins, including antibodies. Directed evolution, which subjects a gene to iterative rounds of mutagenesis, selection and amplification, is useful for protein engineering. In vivo continuous mutagenesis, which continuously...
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Main Authors: | Kaho Kajiwara, Wataru Aoki, Naoki Koike, Mitsuyoshi Ueda |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2021
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Online Access: | https://doaj.org/article/3c96bce5a6a04f1d89df7768632d6aad |
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