Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon ramulosum

Background: Invariant natural killer T cells (iNKT ) can be activated by certain types of glycolipids that have the potential to generate adjuvant effects which could be used to develop effective and safe immunotherapies. Many of these glycolipids have been isolated from natural organisms, but ther...

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Autores principales:	Andres BAENA GARCÍA, Lina GÓMEZ-GIRALDO, Wilton A. GOMEZ HENAO, Carlos Alberto PELAEZ JARAMILLO
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2015
Materias:	iNKT's Glycolipids CD1d Lichens Adjuvant Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/3ca1f0964ca74bfbb35e6ad00124d4ea
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spelling	oai:doaj.org-article:3ca1f0964ca74bfbb35e6ad00124d4ea2021-11-19T04:10:28ZMurine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon ramulosum0121-40042145-2660https://doaj.org/article/3ca1f0964ca74bfbb35e6ad00124d4ea2015-07-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/21794https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 Background: Invariant natural killer T cells (iNKT ) can be activated by certain types of glycolipids that have the potential to generate adjuvant effects which could be used to develop effective and safe immunotherapies. Many of these glycolipids have been isolated from natural organisms, but there is a great amount of these organisms completely unexplored as a source of these types of compounds. Some of these organisms are lichens which are complex symbiotic organisms that have been showed to contain glycolipids. Objectives: We decide to test if glycolipids isolated from lichens would be able to actívate iNKT cells in vitro and in vivo. Methods: We have used extracted glycolipids from 43 different species of lichens from Colombia. We have used iNKT hybridoma cells, C57BL/6 mice, IL-2 ELISA and the B16 melanoma to test for the adjuvant capabilities of glycolipids isolated from lichens. Results: In this study we have found two glycolipids with the capacity to activate iNKT cells in vitro. One of the glycolipids was able to activate iNKT cells in vivo, and was competent to induce protection against the B16 melanoma in the mouse model. Conclusions: We propose a possible chemical structure for a novel glycolipid called β-GalCer-lich (1) derived from the lichen Stereocaulon ramulosum. Andres BAENA GARCÍALina GÓMEZ-GIRALDOWilton A. GOMEZ HENAOCarlos Alberto PELAEZ JARAMILLOUniversidad de AntioquiaarticleiNKT'sGlycolipidsCD1dLichensAdjuvantFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 22, Iss 1 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	iNKT's Glycolipids CD1d Lichens Adjuvant Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	iNKT's Glycolipids CD1d Lichens Adjuvant Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Andres BAENA GARCÍA Lina GÓMEZ-GIRALDO Wilton A. GOMEZ HENAO Carlos Alberto PELAEZ JARAMILLO Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon ramulosum
description	Background: Invariant natural killer T cells (iNKT ) can be activated by certain types of glycolipids that have the potential to generate adjuvant effects which could be used to develop effective and safe immunotherapies. Many of these glycolipids have been isolated from natural organisms, but there is a great amount of these organisms completely unexplored as a source of these types of compounds. Some of these organisms are lichens which are complex symbiotic organisms that have been showed to contain glycolipids. Objectives: We decide to test if glycolipids isolated from lichens would be able to actívate iNKT cells in vitro and in vivo. Methods: We have used extracted glycolipids from 43 different species of lichens from Colombia. We have used iNKT hybridoma cells, C57BL/6 mice, IL-2 ELISA and the B16 melanoma to test for the adjuvant capabilities of glycolipids isolated from lichens. Results: In this study we have found two glycolipids with the capacity to activate iNKT cells in vitro. One of the glycolipids was able to activate iNKT cells in vivo, and was competent to induce protection against the B16 melanoma in the mouse model. Conclusions: We propose a possible chemical structure for a novel glycolipid called β-GalCer-lich (1) derived from the lichen Stereocaulon ramulosum.
format	article
author	Andres BAENA GARCÍA Lina GÓMEZ-GIRALDO Wilton A. GOMEZ HENAO Carlos Alberto PELAEZ JARAMILLO
author_facet	Andres BAENA GARCÍA Lina GÓMEZ-GIRALDO Wilton A. GOMEZ HENAO Carlos Alberto PELAEZ JARAMILLO
author_sort	Andres BAENA GARCÍA
title	Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon ramulosum
title_short	Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon ramulosum
title_full	Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon ramulosum
title_fullStr	Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon ramulosum
title_full_unstemmed	Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon ramulosum
title_sort	murine invariant natural killer t cells recognize glycolipids derived from extracts of the lichen stereocaulon ramulosum
publisher	Universidad de Antioquia
publishDate	2015
url	https://doaj.org/article/3ca1f0964ca74bfbb35e6ad00124d4ea
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Murine invariant natural killer T cells recognize glycolipids derived from extracts of the lichen Stereocaulon ramulosum

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