Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.

Nanotechnology is expected to play a vital role in the rapidly developing field of nanomedicine, creating innovative solutions and therapies for currently untreatable diseases, and providing new tools for various biomedical applications, such as drug delivery and gene therapy. In order to optimize t...

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Autores principales:	Tiago dos Santos, Juan Varela, Iseult Lynch, Anna Salvati, Kenneth A Dawson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ca265fed31a4583b89c9a1c355190af
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spelling	oai:doaj.org-article:3ca265fed31a4583b89c9a1c355190af2021-11-04T06:08:19ZEffects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.1932-620310.1371/journal.pone.0024438https://doaj.org/article/3ca265fed31a4583b89c9a1c355190af2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21949717/?tool=EBIhttps://doaj.org/toc/1932-6203Nanotechnology is expected to play a vital role in the rapidly developing field of nanomedicine, creating innovative solutions and therapies for currently untreatable diseases, and providing new tools for various biomedical applications, such as drug delivery and gene therapy. In order to optimize the efficacy of nanoparticle (NP) delivery to cells, it is necessary to understand the mechanisms by which NPs are internalized by cells, as this will likely determine their ultimate sub-cellular fate and localisation. Here we have used pharmacological inhibitors of some of the major endocytic pathways to investigate nanoparticle uptake mechanisms in a range of representative human cell lines, including HeLa (cervical cancer), A549 (lung carcinoma) and 1321N1 (brain astrocytoma). Chlorpromazine and genistein were used to inhibit clathrin and caveolin mediated endocytosis, respectively. Cytochalasin A and nocodazole were used to inhibit, respectively, the polymerisation of actin and microtubule cytoskeleton. Uptake experiments were performed systematically across the different cell lines, using carboxylated polystyrene NPs of 40 nm and 200 nm diameters, as model NPs of sizes comparable to typical endocytic cargoes. The results clearly indicated that, in all cases and cell types, NPs entered cells via active energy dependent processes. NP uptake in HeLa and 1321N1 cells was strongly affected by actin depolymerisation, while A549 cells showed a stronger inhibition of NP uptake (in comparison to the other cell types) after microtubule disruption and treatment with genistein. A strong reduction of NP uptake was observed after chlorpromazine treatment only in the case of 1321N1 cells. These outcomes suggested that the same NP might exploit different uptake mechanisms to enter different cell types.Tiago dos SantosJuan VarelaIseult LynchAnna SalvatiKenneth A DawsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24438 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tiago dos Santos Juan Varela Iseult Lynch Anna Salvati Kenneth A Dawson Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.
description	Nanotechnology is expected to play a vital role in the rapidly developing field of nanomedicine, creating innovative solutions and therapies for currently untreatable diseases, and providing new tools for various biomedical applications, such as drug delivery and gene therapy. In order to optimize the efficacy of nanoparticle (NP) delivery to cells, it is necessary to understand the mechanisms by which NPs are internalized by cells, as this will likely determine their ultimate sub-cellular fate and localisation. Here we have used pharmacological inhibitors of some of the major endocytic pathways to investigate nanoparticle uptake mechanisms in a range of representative human cell lines, including HeLa (cervical cancer), A549 (lung carcinoma) and 1321N1 (brain astrocytoma). Chlorpromazine and genistein were used to inhibit clathrin and caveolin mediated endocytosis, respectively. Cytochalasin A and nocodazole were used to inhibit, respectively, the polymerisation of actin and microtubule cytoskeleton. Uptake experiments were performed systematically across the different cell lines, using carboxylated polystyrene NPs of 40 nm and 200 nm diameters, as model NPs of sizes comparable to typical endocytic cargoes. The results clearly indicated that, in all cases and cell types, NPs entered cells via active energy dependent processes. NP uptake in HeLa and 1321N1 cells was strongly affected by actin depolymerisation, while A549 cells showed a stronger inhibition of NP uptake (in comparison to the other cell types) after microtubule disruption and treatment with genistein. A strong reduction of NP uptake was observed after chlorpromazine treatment only in the case of 1321N1 cells. These outcomes suggested that the same NP might exploit different uptake mechanisms to enter different cell types.
format	article
author	Tiago dos Santos Juan Varela Iseult Lynch Anna Salvati Kenneth A Dawson
author_facet	Tiago dos Santos Juan Varela Iseult Lynch Anna Salvati Kenneth A Dawson
author_sort	Tiago dos Santos
title	Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.
title_short	Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.
title_full	Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.
title_fullStr	Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.
title_full_unstemmed	Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.
title_sort	effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/3ca265fed31a4583b89c9a1c355190af
work_keys_str_mv	AT tiagodossantos effectsoftransportinhibitorsonthecellularuptakeofcarboxylatedpolystyrenenanoparticlesindifferentcelllines AT juanvarela effectsoftransportinhibitorsonthecellularuptakeofcarboxylatedpolystyrenenanoparticlesindifferentcelllines AT iseultlynch effectsoftransportinhibitorsonthecellularuptakeofcarboxylatedpolystyrenenanoparticlesindifferentcelllines AT annasalvati effectsoftransportinhibitorsonthecellularuptakeofcarboxylatedpolystyrenenanoparticlesindifferentcelllines AT kennethadawson effectsoftransportinhibitorsonthecellularuptakeofcarboxylatedpolystyrenenanoparticlesindifferentcelllines
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Effects of transport inhibitors on the cellular uptake of carboxylated polystyrene nanoparticles in different cell lines.

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