Robust Expression of Functional NMDA Receptors in Human Induced Pluripotent Stem Cell-Derived Neuronal Cultures Using an Accelerated Protocol

N-methyl-D-aspartate (NMDA) receptors are critical for higher-order nervous system function, but in previously published protocols to convert human induced pluripotent stem cells (iPSCs) to mature neurons, functional NMDA receptors (NMDARs) are often either not reported or take an extended time to d...

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Autores principales:	Jacob B. Ruden, Mrinalini Dixit, José C. Zepeda, Brad A. Grueter, Laura L. Dugan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	calcium flux induced pluripotent stem cells (iPSCs) neural progenitor cells (NPCs) neurons NMDA receptors Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/3cabc6151cc24a2b9af9c70870951f28
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spelling	oai:doaj.org-article:3cabc6151cc24a2b9af9c70870951f282021-12-01T07:15:36ZRobust Expression of Functional NMDA Receptors in Human Induced Pluripotent Stem Cell-Derived Neuronal Cultures Using an Accelerated Protocol1662-509910.3389/fnmol.2021.777049https://doaj.org/article/3cabc6151cc24a2b9af9c70870951f282021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnmol.2021.777049/fullhttps://doaj.org/toc/1662-5099N-methyl-D-aspartate (NMDA) receptors are critical for higher-order nervous system function, but in previously published protocols to convert human induced pluripotent stem cells (iPSCs) to mature neurons, functional NMDA receptors (NMDARs) are often either not reported or take an extended time to develop. Here, we describe a protocol to convert human iPSC-derived neural progenitor cells (NPCs) to mature neurons in only 37 days. We demonstrate that the mature neurons express functional NMDARs exhibiting ligand-activated calcium flux, and we document the presence of NMDAR-mediated electrically evoked postsynaptic current. In addition to being more rapid than previous procedures, our protocol is straightforward, does not produce organoids which are difficult to image, and does not involve co-culture with rodent astrocytes. This could enhance our ability to study primate/human-specific aspects of NMDAR function and signaling in health and disease.Jacob B. RudenMrinalini DixitJosé C. ZepedaBrad A. GrueterBrad A. GrueterBrad A. GrueterBrad A. GrueterBrad A. GrueterBrad A. GrueterLaura L. DuganLaura L. DuganLaura L. DuganFrontiers Media S.A.articlecalcium fluxinduced pluripotent stem cells (iPSCs)neural progenitor cells (NPCs)neuronsNMDA receptorsNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Molecular Neuroscience, Vol 14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	calcium flux induced pluripotent stem cells (iPSCs) neural progenitor cells (NPCs) neurons NMDA receptors Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	calcium flux induced pluripotent stem cells (iPSCs) neural progenitor cells (NPCs) neurons NMDA receptors Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Jacob B. Ruden Mrinalini Dixit José C. Zepeda Brad A. Grueter Brad A. Grueter Brad A. Grueter Brad A. Grueter Brad A. Grueter Brad A. Grueter Laura L. Dugan Laura L. Dugan Laura L. Dugan Robust Expression of Functional NMDA Receptors in Human Induced Pluripotent Stem Cell-Derived Neuronal Cultures Using an Accelerated Protocol
description	N-methyl-D-aspartate (NMDA) receptors are critical for higher-order nervous system function, but in previously published protocols to convert human induced pluripotent stem cells (iPSCs) to mature neurons, functional NMDA receptors (NMDARs) are often either not reported or take an extended time to develop. Here, we describe a protocol to convert human iPSC-derived neural progenitor cells (NPCs) to mature neurons in only 37 days. We demonstrate that the mature neurons express functional NMDARs exhibiting ligand-activated calcium flux, and we document the presence of NMDAR-mediated electrically evoked postsynaptic current. In addition to being more rapid than previous procedures, our protocol is straightforward, does not produce organoids which are difficult to image, and does not involve co-culture with rodent astrocytes. This could enhance our ability to study primate/human-specific aspects of NMDAR function and signaling in health and disease.
format	article
author	Jacob B. Ruden Mrinalini Dixit José C. Zepeda Brad A. Grueter Brad A. Grueter Brad A. Grueter Brad A. Grueter Brad A. Grueter Brad A. Grueter Laura L. Dugan Laura L. Dugan Laura L. Dugan
author_facet	Jacob B. Ruden Mrinalini Dixit José C. Zepeda Brad A. Grueter Brad A. Grueter Brad A. Grueter Brad A. Grueter Brad A. Grueter Brad A. Grueter Laura L. Dugan Laura L. Dugan Laura L. Dugan
author_sort	Jacob B. Ruden
title	Robust Expression of Functional NMDA Receptors in Human Induced Pluripotent Stem Cell-Derived Neuronal Cultures Using an Accelerated Protocol
title_short	Robust Expression of Functional NMDA Receptors in Human Induced Pluripotent Stem Cell-Derived Neuronal Cultures Using an Accelerated Protocol
title_full	Robust Expression of Functional NMDA Receptors in Human Induced Pluripotent Stem Cell-Derived Neuronal Cultures Using an Accelerated Protocol
title_fullStr	Robust Expression of Functional NMDA Receptors in Human Induced Pluripotent Stem Cell-Derived Neuronal Cultures Using an Accelerated Protocol
title_full_unstemmed	Robust Expression of Functional NMDA Receptors in Human Induced Pluripotent Stem Cell-Derived Neuronal Cultures Using an Accelerated Protocol
title_sort	robust expression of functional nmda receptors in human induced pluripotent stem cell-derived neuronal cultures using an accelerated protocol
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3cabc6151cc24a2b9af9c70870951f28
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Robust Expression of Functional NMDA Receptors in Human Induced Pluripotent Stem Cell-Derived Neuronal Cultures Using an Accelerated Protocol

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