Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels.

In a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each...

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Autores principales:	Alejandra de-la-Torre, Arnaud Sauer, Alexander W Pfaff, Tristan Bourcier, Julie Brunet, Claude Speeg-Schatz, Laurent Ballonzoli, Odile Villard, Daniel Ajzenberg, Natarajan Sundar, Michael E Grigg, Jorge E Gomez-Marin, Ermanno Candolfi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/3cb184ea2d72493781383585c07fe05c
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spelling	oai:doaj.org-article:3cb184ea2d72493781383585c07fe05c2021-12-02T20:24:12ZSevere South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels.1935-27271935-273510.1371/journal.pntd.0002541https://doaj.org/article/3cb184ea2d72493781383585c07fe05c2013-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278490/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735In a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each patient. The infecting strain was characterized by genotyping when intraocular Toxoplasma DNA was detectable, as well as by peptide-specific serotyping for each patient. To characterize the immune response, we assessed Toxoplasma protein recognition patterns by intraocular antibodies and the intraocular profile of cytokines, chemokines and growth factors. Significant differences were found for size of active lesions, unilateral macular involvement, unilateral visual impairment, vitreous inflammation, synechiae, and vasculitis, with higher values observed throughout for Colombian patients. Multilocus PCR-DNA sequence genotyping was only successful in three Colombian patients revealing one type I and two atypical strains. The Colombian OT patients possessed heterogeneous atypical serotypes whereas the French were uniformly reactive to type II strain peptides. The protein patterns recognized by intraocular antibodies and the cytokine patterns were strikingly different between the two populations. Intraocular IFN-γ and IL-17 expression was lower, while higher levels of IL-13 and IL-6 were detected in aqueous humor of Colombian patients. Our results are consistent with the hypothesis that South American strains may cause more severe OT due to an inhibition of the protective effect of IFN-γ.Alejandra de-la-TorreArnaud SauerAlexander W PfaffTristan BourcierJulie BrunetClaude Speeg-SchatzLaurent BallonzoliOdile VillardDaniel AjzenbergNatarajan SundarMichael E GriggJorge E Gomez-MarinErmanno CandolfiPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 7, Iss 11, p e2541 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alejandra de-la-Torre Arnaud Sauer Alexander W Pfaff Tristan Bourcier Julie Brunet Claude Speeg-Schatz Laurent Ballonzoli Odile Villard Daniel Ajzenberg Natarajan Sundar Michael E Grigg Jorge E Gomez-Marin Ermanno Candolfi Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels.
description	In a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each patient. The infecting strain was characterized by genotyping when intraocular Toxoplasma DNA was detectable, as well as by peptide-specific serotyping for each patient. To characterize the immune response, we assessed Toxoplasma protein recognition patterns by intraocular antibodies and the intraocular profile of cytokines, chemokines and growth factors. Significant differences were found for size of active lesions, unilateral macular involvement, unilateral visual impairment, vitreous inflammation, synechiae, and vasculitis, with higher values observed throughout for Colombian patients. Multilocus PCR-DNA sequence genotyping was only successful in three Colombian patients revealing one type I and two atypical strains. The Colombian OT patients possessed heterogeneous atypical serotypes whereas the French were uniformly reactive to type II strain peptides. The protein patterns recognized by intraocular antibodies and the cytokine patterns were strikingly different between the two populations. Intraocular IFN-γ and IL-17 expression was lower, while higher levels of IL-13 and IL-6 were detected in aqueous humor of Colombian patients. Our results are consistent with the hypothesis that South American strains may cause more severe OT due to an inhibition of the protective effect of IFN-γ.
format	article
author	Alejandra de-la-Torre Arnaud Sauer Alexander W Pfaff Tristan Bourcier Julie Brunet Claude Speeg-Schatz Laurent Ballonzoli Odile Villard Daniel Ajzenberg Natarajan Sundar Michael E Grigg Jorge E Gomez-Marin Ermanno Candolfi
author_facet	Alejandra de-la-Torre Arnaud Sauer Alexander W Pfaff Tristan Bourcier Julie Brunet Claude Speeg-Schatz Laurent Ballonzoli Odile Villard Daniel Ajzenberg Natarajan Sundar Michael E Grigg Jorge E Gomez-Marin Ermanno Candolfi
author_sort	Alejandra de-la-Torre
title	Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels.
title_short	Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels.
title_full	Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels.
title_fullStr	Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels.
title_full_unstemmed	Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels.
title_sort	severe south american ocular toxoplasmosis is associated with decreased ifn-γ/il-17a and increased il-6/il-13 intraocular levels.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3cb184ea2d72493781383585c07fe05c
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Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels.

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