Minocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages

Minocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages

D Liu, P S YangShandong Provincial Key Laboratory of Oral Biomedicine, College of Stomatology, Shandong University, Shandong Province, People's Republic of ChinaBackground: As an adjunctive treatment of chronic periodontitis, it seems that the application of periocline or the other antim...

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Autores principales: Liu D, Yang PS
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/3cb258ad134e4c72be126ea8ea1bfbdc
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spelling oai:doaj.org-article:3cb258ad134e4c72be126ea8ea1bfbdc2021-12-02T08:20:22ZMinocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages1176-91141178-2013https://doaj.org/article/3cb258ad134e4c72be126ea8ea1bfbdc2012-08-01T00:00:00Zhttp://www.dovepress.com/minocycline-hydrochloride-nanoliposomes-inhibit-the-production-of-tnf--a10875https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013D Liu, P S YangShandong Provincial Key Laboratory of Oral Biomedicine, College of Stomatology, Shandong University, Shandong Province, People's Republic of ChinaBackground: As an adjunctive treatment of chronic periodontitis, it seems that the application of periocline or the other antimicrobials is effective against periodontopathogens. In this study, nanoliposomes were investigated as carriers of minocycline hydrochloride and the inhibition effects of minocycline hydrochloride nanoliposomes on the proliferation and lipopolysaccharide (LPS)-stimulated production of tumor necrosis factor-α (TNF-α) of macrophages were elucidated.Methods: After stimulation with 10 µg/mL LPS, murine macrophages (ANA-1) were treated with 10, 20, 40, 50 and 70 µg/mL 2% minocycline hydrochloride nanoliposomes, minocycline hydrochloride solution, and periocline for 6, 12, 24, 48 and 60 hours, respectively. A tetrazolium (MTT) assay was used to evaluate macrophages cell proliferation rate and the levels of TNF-α mRNA were measured by SYBR Green Real Time PCR.Results: Ten to 70 µg/mL 2% minocycline hydrochloride nanoliposomes, minocycline hydrochloride solution, and periocline showed dose- and time-dependent inhibition of ANA-1 proliferation. Minocycline hydrochloride nanoliposomes showed dose- and ratio-dependent inhibition of LPS-stimulated TNF-α secretion of ANA-1. The inhibition effect of 10 µg/mL minocycline hydrochloride nanoliposomes was significantly better than that of two positive control groups, and equated to that of 60 or 70 µg/mL periocline. The expression of TNF-α mRNA in experimental group continued to reduce linearly with time.Conclusion: All three preparations of minocycline hydrochloride showed dose- and time-dependent inhibition of proliferation of ANA-1. Minocycline hydrochloride nanoliposomes have stronger and longer inhibition effect on LPS-stimulated TNF-α secretion of macrophages cell than minocycline hydrochloride solution and periocline.Keywords: minocycline hydrochloride, nanoliposomes, periodontitis, lipopolysaccharide, macrophages, tumor necrosis factor-αLiu DYang PSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4769-4775 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Liu D
Yang PS
Minocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages
description D Liu, P S YangShandong Provincial Key Laboratory of Oral Biomedicine, College of Stomatology, Shandong University, Shandong Province, People's Republic of ChinaBackground: As an adjunctive treatment of chronic periodontitis, it seems that the application of periocline or the other antimicrobials is effective against periodontopathogens. In this study, nanoliposomes were investigated as carriers of minocycline hydrochloride and the inhibition effects of minocycline hydrochloride nanoliposomes on the proliferation and lipopolysaccharide (LPS)-stimulated production of tumor necrosis factor-α (TNF-α) of macrophages were elucidated.Methods: After stimulation with 10 µg/mL LPS, murine macrophages (ANA-1) were treated with 10, 20, 40, 50 and 70 µg/mL 2% minocycline hydrochloride nanoliposomes, minocycline hydrochloride solution, and periocline for 6, 12, 24, 48 and 60 hours, respectively. A tetrazolium (MTT) assay was used to evaluate macrophages cell proliferation rate and the levels of TNF-α mRNA were measured by SYBR Green Real Time PCR.Results: Ten to 70 µg/mL 2% minocycline hydrochloride nanoliposomes, minocycline hydrochloride solution, and periocline showed dose- and time-dependent inhibition of ANA-1 proliferation. Minocycline hydrochloride nanoliposomes showed dose- and ratio-dependent inhibition of LPS-stimulated TNF-α secretion of ANA-1. The inhibition effect of 10 µg/mL minocycline hydrochloride nanoliposomes was significantly better than that of two positive control groups, and equated to that of 60 or 70 µg/mL periocline. The expression of TNF-α mRNA in experimental group continued to reduce linearly with time.Conclusion: All three preparations of minocycline hydrochloride showed dose- and time-dependent inhibition of proliferation of ANA-1. Minocycline hydrochloride nanoliposomes have stronger and longer inhibition effect on LPS-stimulated TNF-α secretion of macrophages cell than minocycline hydrochloride solution and periocline.Keywords: minocycline hydrochloride, nanoliposomes, periodontitis, lipopolysaccharide, macrophages, tumor necrosis factor-α
format article
author Liu D
Yang PS
author_facet Liu D
Yang PS
author_sort Liu D
title Minocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages
title_short Minocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages
title_full Minocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages
title_fullStr Minocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages
title_full_unstemmed Minocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages
title_sort minocycline hydrochloride nanoliposomes inhibit the production of tnf-α in lps-stimulated macrophages
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/3cb258ad134e4c72be126ea8ea1bfbdc
work_keys_str_mv AT liud minocyclinehydrochloridenanoliposomesinhibittheproductionoftnfampalphainlpsstimulatedmacrophages
AT yangps minocyclinehydrochloridenanoliposomesinhibittheproductionoftnfampalphainlpsstimulatedmacrophages
_version_ 1718398538071670784

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