Behavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos/larvae (Danio rerio)

Strobilurin fungicides are among the most widely used in the world and have characteristics that include high water solubility and toxicity to aquatic organisms. While several studies report on mechanisms of toxicity of strobilurins in fish, there are no data on the sub-lethal toxicity of fish to th...

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Autores principales:	Tao Huang, Christopher L. Souders, II, Shuo Wang, Jade Ganter, Jia He, Yuan H. Zhao, Hongguang Cheng, Christopher J. Martyniuk
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Strobilurins Fenamidone Development toxicity Mitochondrial oxygen consumption rates Locomotor activity Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/3cb4d56c80434f53803c6406488a70dc
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spelling	oai:doaj.org-article:3cb4d56c80434f53803c6406488a70dc2021-11-18T04:43:15ZBehavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos/larvae (Danio rerio)0147-651310.1016/j.ecoenv.2021.112966https://doaj.org/article/3cb4d56c80434f53803c6406488a70dc2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010782https://doaj.org/toc/0147-6513Strobilurin fungicides are among the most widely used in the world and have characteristics that include high water solubility and toxicity to aquatic organisms. While several studies report on mechanisms of toxicity of strobilurins in fish, there are no data on the sub-lethal toxicity of fish to the fungicide fenamidone. To address this gap, survival and hatch rate, deformities, mitochondrial bioenergetics, expression of oxidative stress and apoptotic genes, and behavior (locomotor activity and anxiolytic-related behaviors) were assessed in zebrafish embryos and larvae following exposure to fenamidone. Fenamidone negatively affected development of zebrafish embryos, causing a delay of hatching time at concentrations of 2.5 and 5 μM. Fenamidone caused morphological deformities in zebrafish, including pericardial edema, yolk sac edema, tail deformities, and spinal curvature. Exposure to 1.5 μM fenamidone reduced surface area of swim bladder in larvae at 6 dpf. Fenamidone significantly reduced oxygen consumption rates of embryos; 5 μM fenamidone decreased basal respiration (~85%), oligomycin induced ATP-linked respiration (~70%), FCCP-induced maximal respiration (~75%) and non-mitochondrial respiration (~90%) compared to controls. Sod2 mRNA levels were decreased by fenamidone in larval fish. Locomotor activity was significantly decreased in zebrafish larvae following exposure to 2 μM fenamidone but there was no evidence for anxiolytic nor anxiety-related behaviors (exposures of 100 nM up to 1.5 µM). This study addresses a data gap for potential risks associated with fenamidone exposure in developing fish.Tao HuangChristopher L. Souders, IIShuo WangJade GanterJia HeYuan H. ZhaoHongguang ChengChristopher J. MartyniukElsevierarticleStrobilurinsFenamidoneDevelopment toxicityMitochondrial oxygen consumption ratesLocomotor activityEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 112966- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Strobilurins Fenamidone Development toxicity Mitochondrial oxygen consumption rates Locomotor activity Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Strobilurins Fenamidone Development toxicity Mitochondrial oxygen consumption rates Locomotor activity Environmental pollution TD172-193.5 Environmental sciences GE1-350 Tao Huang Christopher L. Souders, II Shuo Wang Jade Ganter Jia He Yuan H. Zhao Hongguang Cheng Christopher J. Martyniuk Behavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos/larvae (Danio rerio)
description	Strobilurin fungicides are among the most widely used in the world and have characteristics that include high water solubility and toxicity to aquatic organisms. While several studies report on mechanisms of toxicity of strobilurins in fish, there are no data on the sub-lethal toxicity of fish to the fungicide fenamidone. To address this gap, survival and hatch rate, deformities, mitochondrial bioenergetics, expression of oxidative stress and apoptotic genes, and behavior (locomotor activity and anxiolytic-related behaviors) were assessed in zebrafish embryos and larvae following exposure to fenamidone. Fenamidone negatively affected development of zebrafish embryos, causing a delay of hatching time at concentrations of 2.5 and 5 μM. Fenamidone caused morphological deformities in zebrafish, including pericardial edema, yolk sac edema, tail deformities, and spinal curvature. Exposure to 1.5 μM fenamidone reduced surface area of swim bladder in larvae at 6 dpf. Fenamidone significantly reduced oxygen consumption rates of embryos; 5 μM fenamidone decreased basal respiration (~85%), oligomycin induced ATP-linked respiration (~70%), FCCP-induced maximal respiration (~75%) and non-mitochondrial respiration (~90%) compared to controls. Sod2 mRNA levels were decreased by fenamidone in larval fish. Locomotor activity was significantly decreased in zebrafish larvae following exposure to 2 μM fenamidone but there was no evidence for anxiolytic nor anxiety-related behaviors (exposures of 100 nM up to 1.5 µM). This study addresses a data gap for potential risks associated with fenamidone exposure in developing fish.
format	article
author	Tao Huang Christopher L. Souders, II Shuo Wang Jade Ganter Jia He Yuan H. Zhao Hongguang Cheng Christopher J. Martyniuk
author_facet	Tao Huang Christopher L. Souders, II Shuo Wang Jade Ganter Jia He Yuan H. Zhao Hongguang Cheng Christopher J. Martyniuk
author_sort	Tao Huang
title	Behavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos/larvae (Danio rerio)
title_short	Behavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos/larvae (Danio rerio)
title_full	Behavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos/larvae (Danio rerio)
title_fullStr	Behavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos/larvae (Danio rerio)
title_full_unstemmed	Behavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos/larvae (Danio rerio)
title_sort	behavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos/larvae (danio rerio)
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/3cb4d56c80434f53803c6406488a70dc
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Behavioral and developmental toxicity assessment of the strobilurin fungicide fenamidone in zebrafish embryos/larvae (Danio rerio)

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