Deep Sequencing Identifies Noncanonical Editing of Ebola and Marburg Virus RNAs in Infected Cells

ABSTRACT Deep sequencing of RNAs produced by Zaire ebolavirus (EBOV) or the Angola strain of Marburgvirus (MARV-Ang) identified novel viral and cellular mechanisms that diversify the coding and noncoding sequences of viral mRNAs and genomic RNAs. We identified previously undescribed sites within the...

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Autores principales:	Reed S. Shabman, Omar J. Jabado, Chad E. Mire, Timothy B. Stockwell, Megan Edwards, Milind Mahajan, Thomas W. Geisbert, Christopher F. Basler
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3cbce08f99e9408999873963711c686b
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spelling	oai:doaj.org-article:3cbce08f99e9408999873963711c686b2021-11-15T15:47:03ZDeep Sequencing Identifies Noncanonical Editing of Ebola and Marburg Virus RNAs in Infected Cells10.1128/mBio.02011-142150-7511https://doaj.org/article/3cbce08f99e9408999873963711c686b2014-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02011-14https://doaj.org/toc/2150-7511ABSTRACT Deep sequencing of RNAs produced by Zaire ebolavirus (EBOV) or the Angola strain of Marburgvirus (MARV-Ang) identified novel viral and cellular mechanisms that diversify the coding and noncoding sequences of viral mRNAs and genomic RNAs. We identified previously undescribed sites within the EBOV and MARV-Ang mRNAs where apparent cotranscriptional editing has resulted in the addition of non-template-encoded residues within the EBOV glycoprotein (GP) mRNA, the MARV-Ang nucleoprotein (NP) mRNA, and the MARV-Ang polymerase (L) mRNA, such that novel viral translation products could be produced. Further, we found that the well-characterized EBOV GP mRNA editing site is modified at a high frequency during viral genome RNA replication. Additionally, editing hot spots representing sites of apparent adenosine deaminase activity were found in the MARV-Ang NP 3′-untranslated region. These studies identify novel filovirus-host interactions and reveal production of a greater diversity of filoviral gene products than was previously appreciated. IMPORTANCE This study identifies novel mechanisms that alter the protein coding capacities of Ebola and Marburg virus mRNAs. Therefore, filovirus gene expression is more complex and diverse than previously recognized. These observations suggest new directions in understanding the regulation of filovirus gene expression.Reed S. ShabmanOmar J. JabadoChad E. MireTimothy B. StockwellMegan EdwardsMilind MahajanThomas W. GeisbertChristopher F. BaslerAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 6 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Reed S. Shabman Omar J. Jabado Chad E. Mire Timothy B. Stockwell Megan Edwards Milind Mahajan Thomas W. Geisbert Christopher F. Basler Deep Sequencing Identifies Noncanonical Editing of Ebola and Marburg Virus RNAs in Infected Cells
description	ABSTRACT Deep sequencing of RNAs produced by Zaire ebolavirus (EBOV) or the Angola strain of Marburgvirus (MARV-Ang) identified novel viral and cellular mechanisms that diversify the coding and noncoding sequences of viral mRNAs and genomic RNAs. We identified previously undescribed sites within the EBOV and MARV-Ang mRNAs where apparent cotranscriptional editing has resulted in the addition of non-template-encoded residues within the EBOV glycoprotein (GP) mRNA, the MARV-Ang nucleoprotein (NP) mRNA, and the MARV-Ang polymerase (L) mRNA, such that novel viral translation products could be produced. Further, we found that the well-characterized EBOV GP mRNA editing site is modified at a high frequency during viral genome RNA replication. Additionally, editing hot spots representing sites of apparent adenosine deaminase activity were found in the MARV-Ang NP 3′-untranslated region. These studies identify novel filovirus-host interactions and reveal production of a greater diversity of filoviral gene products than was previously appreciated. IMPORTANCE This study identifies novel mechanisms that alter the protein coding capacities of Ebola and Marburg virus mRNAs. Therefore, filovirus gene expression is more complex and diverse than previously recognized. These observations suggest new directions in understanding the regulation of filovirus gene expression.
format	article
author	Reed S. Shabman Omar J. Jabado Chad E. Mire Timothy B. Stockwell Megan Edwards Milind Mahajan Thomas W. Geisbert Christopher F. Basler
author_facet	Reed S. Shabman Omar J. Jabado Chad E. Mire Timothy B. Stockwell Megan Edwards Milind Mahajan Thomas W. Geisbert Christopher F. Basler
author_sort	Reed S. Shabman
title	Deep Sequencing Identifies Noncanonical Editing of Ebola and Marburg Virus RNAs in Infected Cells
title_short	Deep Sequencing Identifies Noncanonical Editing of Ebola and Marburg Virus RNAs in Infected Cells
title_full	Deep Sequencing Identifies Noncanonical Editing of Ebola and Marburg Virus RNAs in Infected Cells
title_fullStr	Deep Sequencing Identifies Noncanonical Editing of Ebola and Marburg Virus RNAs in Infected Cells
title_full_unstemmed	Deep Sequencing Identifies Noncanonical Editing of Ebola and Marburg Virus RNAs in Infected Cells
title_sort	deep sequencing identifies noncanonical editing of ebola and marburg virus rnas in infected cells
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/3cbce08f99e9408999873963711c686b
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Deep Sequencing Identifies Noncanonical Editing of Ebola and Marburg Virus RNAs in Infected Cells

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