Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity

Herawati W, Amurwanto A, Nafi’ah Z, Ningrum AM, Samiyarsih S. 2018. Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity. Biodiversitas 19: 119-125. A variation analysis of three salak cultivars was conducted in Banyumas District, C...

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Autores principales:	WIWIK HERAWATI, ADI AMURWANTO, ZUHROTUN NAFIâ€™AH, ARDIANTI MAYA NINGRUM, SITI SAMIYARSIH
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	anatomical characteristics molecular snake fruit Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3cbf00a44a914e12afa4ef128007bd78
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spelling	oai:doaj.org-article:3cbf00a44a914e12afa4ef128007bd782021-11-16T14:01:05ZVariation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity1412-033X2085-472210.13057/biodiv/d190118https://doaj.org/article/3cbf00a44a914e12afa4ef128007bd782018-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2342https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Herawati W, Amurwanto A, Nafi’ah Z, Ningrum AM, Samiyarsih S. 2018. Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity. Biodiversitas 19: 119-125. A variation analysis of three salak cultivars was conducted in Banyumas District, Central Java, Indonesia. The aim of the study was to investigate variation amongst the cultivars based on anatomy and genetic diversity by using RAPD (Random Amplified Polymorphic DNA) technique. The result of the study showed that all salak cultivars have similar variation in leaf anatomy. The only distinguishing characteristic is the number of trichomes on cultivars Kedung Paruk and Kalisube per 1 mm2 unit of leaf area, while the cultivars of Candinegara lacked trichomes. Ten primers could detect 50 polymorphic bands (80,6%) from a total of 62 bands which could be observed clearly. The percentage of polymorphic loci in 'Candinegara' was 64%, 'Kedung Paruk' 62%, and 'Kalisube' 44%. The heterozygosity value of 'Candinegara' was 0.1590, 'Kedung Paruk' 0,1449, and 'Candinegara' (0.0235). Meanwhile, the furthest genetic distance was shown by 'Kedung Paruk' and ‘Candinegara’ (0.0704). This result gives us important basic information about cultivars of Salacca zalacca in Banyumas District which have high genetic diversity and germplasm. These results can be used for further research such as for conservation and genetic engineering.WIWIK HERAWATIADI AMURWANTOZUHROTUN NAFIâ€™AHARDIANTI MAYA NINGRUMSITI SAMIYARSIHMBI & UNS Soloarticleanatomical characteristicsmolecularsnake fruitBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 1, Pp 119-125 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	anatomical characteristics molecular snake fruit Biology (General) QH301-705.5
spellingShingle	anatomical characteristics molecular snake fruit Biology (General) QH301-705.5 WIWIK HERAWATI ADI AMURWANTO ZUHROTUN NAFIâ€™AH ARDIANTI MAYA NINGRUM SITI SAMIYARSIH Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity
description	Herawati W, Amurwanto A, Nafi’ah Z, Ningrum AM, Samiyarsih S. 2018. Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity. Biodiversitas 19: 119-125. A variation analysis of three salak cultivars was conducted in Banyumas District, Central Java, Indonesia. The aim of the study was to investigate variation amongst the cultivars based on anatomy and genetic diversity by using RAPD (Random Amplified Polymorphic DNA) technique. The result of the study showed that all salak cultivars have similar variation in leaf anatomy. The only distinguishing characteristic is the number of trichomes on cultivars Kedung Paruk and Kalisube per 1 mm2 unit of leaf area, while the cultivars of Candinegara lacked trichomes. Ten primers could detect 50 polymorphic bands (80,6%) from a total of 62 bands which could be observed clearly. The percentage of polymorphic loci in 'Candinegara' was 64%, 'Kedung Paruk' 62%, and 'Kalisube' 44%. The heterozygosity value of 'Candinegara' was 0.1590, 'Kedung Paruk' 0,1449, and 'Candinegara' (0.0235). Meanwhile, the furthest genetic distance was shown by 'Kedung Paruk' and ‘Candinegara’ (0.0704). This result gives us important basic information about cultivars of Salacca zalacca in Banyumas District which have high genetic diversity and germplasm. These results can be used for further research such as for conservation and genetic engineering.
format	article
author	WIWIK HERAWATI ADI AMURWANTO ZUHROTUN NAFIâ€™AH ARDIANTI MAYA NINGRUM SITI SAMIYARSIH
author_facet	WIWIK HERAWATI ADI AMURWANTO ZUHROTUN NAFIâ€™AH ARDIANTI MAYA NINGRUM SITI SAMIYARSIH
author_sort	WIWIK HERAWATI
title	Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity
title_short	Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity
title_full	Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity
title_fullStr	Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity
title_full_unstemmed	Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity
title_sort	variation analysis of three banyumas local salak cultivars (salacca zalacca) based on leaf anatomy and genetic diversity
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/3cbf00a44a914e12afa4ef128007bd78
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Variation analysis of three Banyumas local salak cultivars (Salacca zalacca) based on leaf anatomy and genetic diversity

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