The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis

Abstract Monocytes/macrophages have been found to be an important component of colitis. However, the key chemokine that initiates the CCR2+ monocytes migration from circulation to colitis tissue remains to be undiscovered. PC3-secreted microprotein (PSMP) is a novel chemokine whose receptor is CCR2....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaolei Pei, Danfeng Zheng, Shaoping She, Jing Ma, Changyuan Guo, Xiaoning Mo, Yingmei Zhang, Quansheng Song, Yu Zhang, Dalong Ma, Ying Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3cbfbbfc63c74b4a87367152c720eb02
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3cbfbbfc63c74b4a87367152c720eb02
record_format	dspace
spelling	oai:doaj.org-article:3cbfbbfc63c74b4a87367152c720eb022021-12-02T11:52:39ZThe PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis10.1038/s41598-017-05255-72045-2322https://doaj.org/article/3cbfbbfc63c74b4a87367152c720eb022017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05255-7https://doaj.org/toc/2045-2322Abstract Monocytes/macrophages have been found to be an important component of colitis. However, the key chemokine that initiates the CCR2+ monocytes migration from circulation to colitis tissue remains to be undiscovered. PC3-secreted microprotein (PSMP) is a novel chemokine whose receptor is CCR2. The physiological and pathological functions of PSMP have not yet been reported. In this study, PSMP was found to be expressed in colitis and colonic tumor tissues from patients and significantly up-regulated in mouse DSS-induced colitis tissues. PSMP overexpression in the colon aggravated the DSS-induced colitis and the anti-PSMP neutralizing antibody mollified the colitis by reducing macrophage infiltration and inhibiting the expression of IL-6, TNF-α and CCL2. Furthermore, we demonstrated that lipopolysaccharide and muramyl dipeptide induced PSMP expression in the colonic epithelial cells. PSMP was up-regulated in the initial stage prior to IL-6, TNF-α and CCL2 up-regulated expression in DSS colitis and promoted the M1 macrophages to produce CCL2. PSMP chemo-attracted Ly6Chi monocytes in a CCR2 dependent manner via in situ chemotaxis and adoptive transfer assays. Our data identify PSMP as a key molecule in ulcerative colitis, which provides a novel mechanism of monocyte/macrophage migration that affects gut innate immunity and makes PSMP a potential target for controlling colitis.Xiaolei PeiDanfeng ZhengShaoping SheJing MaChangyuan GuoXiaoning MoYingmei ZhangQuansheng SongYu ZhangDalong MaYing WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaolei Pei Danfeng Zheng Shaoping She Jing Ma Changyuan Guo Xiaoning Mo Yingmei Zhang Quansheng Song Yu Zhang Dalong Ma Ying Wang The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis
description	Abstract Monocytes/macrophages have been found to be an important component of colitis. However, the key chemokine that initiates the CCR2+ monocytes migration from circulation to colitis tissue remains to be undiscovered. PC3-secreted microprotein (PSMP) is a novel chemokine whose receptor is CCR2. The physiological and pathological functions of PSMP have not yet been reported. In this study, PSMP was found to be expressed in colitis and colonic tumor tissues from patients and significantly up-regulated in mouse DSS-induced colitis tissues. PSMP overexpression in the colon aggravated the DSS-induced colitis and the anti-PSMP neutralizing antibody mollified the colitis by reducing macrophage infiltration and inhibiting the expression of IL-6, TNF-α and CCL2. Furthermore, we demonstrated that lipopolysaccharide and muramyl dipeptide induced PSMP expression in the colonic epithelial cells. PSMP was up-regulated in the initial stage prior to IL-6, TNF-α and CCL2 up-regulated expression in DSS colitis and promoted the M1 macrophages to produce CCL2. PSMP chemo-attracted Ly6Chi monocytes in a CCR2 dependent manner via in situ chemotaxis and adoptive transfer assays. Our data identify PSMP as a key molecule in ulcerative colitis, which provides a novel mechanism of monocyte/macrophage migration that affects gut innate immunity and makes PSMP a potential target for controlling colitis.
format	article
author	Xiaolei Pei Danfeng Zheng Shaoping She Jing Ma Changyuan Guo Xiaoning Mo Yingmei Zhang Quansheng Song Yu Zhang Dalong Ma Ying Wang
author_facet	Xiaolei Pei Danfeng Zheng Shaoping She Jing Ma Changyuan Guo Xiaoning Mo Yingmei Zhang Quansheng Song Yu Zhang Dalong Ma Ying Wang
author_sort	Xiaolei Pei
title	The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis
title_short	The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis
title_full	The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis
title_fullStr	The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis
title_full_unstemmed	The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis
title_sort	psmp-ccr2 interactions trigger monocyte/macrophage-dependent colitis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3cbfbbfc63c74b4a87367152c720eb02
work_keys_str_mv	AT xiaoleipei thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT danfengzheng thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT shaopingshe thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT jingma thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT changyuanguo thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT xiaoningmo thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT yingmeizhang thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT quanshengsong thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT yuzhang thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT dalongma thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT yingwang thepsmpccr2interactionstriggermonocytemacrophagedependentcolitis AT xiaoleipei psmpccr2interactionstriggermonocytemacrophagedependentcolitis AT danfengzheng psmpccr2interactionstriggermonocytemacrophagedependentcolitis AT shaopingshe psmpccr2interactionstriggermonocytemacrophagedependentcolitis AT jingma psmpccr2interactionstriggermonocytemacrophagedependentcolitis AT changyuanguo psmpccr2interactionstriggermonocytemacrophagedependentcolitis AT xiaoningmo psmpccr2interactionstriggermonocytemacrophagedependentcolitis AT yingmeizhang psmpccr2interactionstriggermonocytemacrophagedependentcolitis AT quanshengsong psmpccr2interactionstriggermonocytemacrophagedependentcolitis AT yuzhang psmpccr2interactionstriggermonocytemacrophagedependentcolitis AT dalongma psmpccr2interactionstriggermonocytemacrophagedependentcolitis AT yingwang psmpccr2interactionstriggermonocytemacrophagedependentcolitis
_version_	1718394978442412032

The PSMP-CCR2 interactions trigger monocyte/macrophage-dependent colitis

Ejemplares similares