Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone

Savitha Nadig1, Nithya Velusamy2, Prajna Lalitha2, Sarita Kar3, Savitri Sharma3, Gayathri Arakere11Society for Innovation and Development, Indian Institute of Science, Bengaluru, Karnataka, 2Aravind Eye Hospital, Madurai, Tamil Nadu, 3LV Prasad Eye Institute, Bhubaneswar, Odisha, IndiaPurpose: The p...

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Autores principales:	Nadig S, Velusamy N, Lalitha P, Kar S, Sharma S, Arakere G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/3cc361f8b6cb4e7aabe9afff0314dbf4
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spelling	oai:doaj.org-article:3cc361f8b6cb4e7aabe9afff0314dbf42021-12-02T05:33:06ZStaphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone1177-54671177-5483https://doaj.org/article/3cc361f8b6cb4e7aabe9afff0314dbf42012-01-01T00:00:00Zhttp://www.dovepress.com/staphylococcus-aureus-eye-infections-in-two-indian-hospitals-emergence-a9118https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Savitha Nadig1, Nithya Velusamy2, Prajna Lalitha2, Sarita Kar3, Savitri Sharma3, Gayathri Arakere11Society for Innovation and Development, Indian Institute of Science, Bengaluru, Karnataka, 2Aravind Eye Hospital, Madurai, Tamil Nadu, 3LV Prasad Eye Institute, Bhubaneswar, Odisha, IndiaPurpose: The purpose of this study was to perform molecular characterization of Staphylococcus aureus isolates causing a variety of eye infections from two major eye care hospitals in India.Methods: Twenty-four isolates from Aravind Eye Hospital, Madurai, India, and nine isolates from LV Prasad Eye Institute, Bhubaneswar, India, representing severe to nonsevere eye infections like microbial keratitis to lacrimal sac abscess, were characterized. Staphylococcal cassette chromosome mec typing, multilocus sequence typing, accessory gene regulator typing, staphylococcal protein A typing, and pulsed field gel electrophoresis were used, along with determination of the presence of Panton&ndash;Valentine leucocidin toxin and endotoxin gene cluster among each sequence type.Results: The majority of eye infections, both severe and nonsevere, were caused by sequence type (ST)772, positive for the Panton&ndash;Valentine leucocidin gene, and carrying methicillin-resistant staphylococcal cassette chromosome mec type V cassette (22/33, 67%). Some of the other sequence types that caused severe eye infections were ST1 (9%), 5 (3%), 72 (6%), 88 (3%), 121 (3%), and 672 (3%). This is the first report of the presence of ST1 and 88 in India.Conclusion: Although the number of isolates included in this study was small, most of the eye infections were caused by community-associated S. aureus where patients had no history of hospitalization or treatment in the past year. In the case of six severe infections, patients were admitted for surgeries and there is probability of hospital infection. In addition, only methicillin-resistant S. aureus isolates carrying staphylococcal cassette chromosome mec type V were detected. Epidemic methicillin-resistant Staphylococcus aureus 15 (ST22) is a major ST found in health care as well as community settings in non-eye infections in India, but only one methicillin-sensitive S. aureus isolate belonging to ST22 was detected. Predominantly ST772, along with a few other STs, caused the 33 eye infections studied.Keywords: CA-MRSA, severe and nonsevere eye infections, ST772, PVL, agr type IINadig SVelusamy NLalitha PKar SSharma SArakere GDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2012, Iss default, Pp 165-173 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Nadig S Velusamy N Lalitha P Kar S Sharma S Arakere G Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone
description	Savitha Nadig1, Nithya Velusamy2, Prajna Lalitha2, Sarita Kar3, Savitri Sharma3, Gayathri Arakere11Society for Innovation and Development, Indian Institute of Science, Bengaluru, Karnataka, 2Aravind Eye Hospital, Madurai, Tamil Nadu, 3LV Prasad Eye Institute, Bhubaneswar, Odisha, IndiaPurpose: The purpose of this study was to perform molecular characterization of Staphylococcus aureus isolates causing a variety of eye infections from two major eye care hospitals in India.Methods: Twenty-four isolates from Aravind Eye Hospital, Madurai, India, and nine isolates from LV Prasad Eye Institute, Bhubaneswar, India, representing severe to nonsevere eye infections like microbial keratitis to lacrimal sac abscess, were characterized. Staphylococcal cassette chromosome mec typing, multilocus sequence typing, accessory gene regulator typing, staphylococcal protein A typing, and pulsed field gel electrophoresis were used, along with determination of the presence of Panton&ndash;Valentine leucocidin toxin and endotoxin gene cluster among each sequence type.Results: The majority of eye infections, both severe and nonsevere, were caused by sequence type (ST)772, positive for the Panton&ndash;Valentine leucocidin gene, and carrying methicillin-resistant staphylococcal cassette chromosome mec type V cassette (22/33, 67%). Some of the other sequence types that caused severe eye infections were ST1 (9%), 5 (3%), 72 (6%), 88 (3%), 121 (3%), and 672 (3%). This is the first report of the presence of ST1 and 88 in India.Conclusion: Although the number of isolates included in this study was small, most of the eye infections were caused by community-associated S. aureus where patients had no history of hospitalization or treatment in the past year. In the case of six severe infections, patients were admitted for surgeries and there is probability of hospital infection. In addition, only methicillin-resistant S. aureus isolates carrying staphylococcal cassette chromosome mec type V were detected. Epidemic methicillin-resistant Staphylococcus aureus 15 (ST22) is a major ST found in health care as well as community settings in non-eye infections in India, but only one methicillin-sensitive S. aureus isolate belonging to ST22 was detected. Predominantly ST772, along with a few other STs, caused the 33 eye infections studied.Keywords: CA-MRSA, severe and nonsevere eye infections, ST772, PVL, agr type II
format	article
author	Nadig S Velusamy N Lalitha P Kar S Sharma S Arakere G
author_facet	Nadig S Velusamy N Lalitha P Kar S Sharma S Arakere G
author_sort	Nadig S
title	Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone
title_short	Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone
title_full	Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone
title_fullStr	Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone
title_full_unstemmed	Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone
title_sort	staphylococcus aureus eye infections in two indian hospitals: emergence of st772 as a major clone
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/3cc361f8b6cb4e7aabe9afff0314dbf4
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Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone

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