Lysophosphatidic Acid Alters The Expression of Apoptosis Related Genes and miR-22 in Cultured and Autotransplanted Ovaries

Objective: The aim of this study was to evaluate the effect of lysophosphatidic acid (LPA) on the follicular development, incidence of cell death, and expressions of apoptosis related genes and miR-22 in transplanted ovaries. Materials and Methods: In this experimental study, three-week-old mice ov...

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Autores principales:	Maryam Dehghan, Shirin Shahbazi, Mojdeh Salehnia
Formato:	article
Lenguaje:	EN
Publicado:	Royan Institute (ACECR), Tehran 2021
Materias:	apoptosis autotransplantation bax protein lysophosphatidic acid ovarian follicle Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3cc7e31e3211490181c7cdc549ea5afc
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spelling	oai:doaj.org-article:3cc7e31e3211490181c7cdc549ea5afc2021-11-07T08:07:05ZLysophosphatidic Acid Alters The Expression of Apoptosis Related Genes and miR-22 in Cultured and Autotransplanted Ovaries10.22074/cellj.2021.73032228-58062228-5814https://doaj.org/article/3cc7e31e3211490181c7cdc549ea5afc2021-10-01T00:00:00Zhttps://celljournal.org/journal/article/fulltext/effects-of-adipose-derived-stem-cells-and-platelet-rich-plasma-exosomes-on-the-inductivity-of-hair-dermal-papilla-cells.pdfhttps://doaj.org/toc/2228-5806https://doaj.org/toc/2228-5814Objective: The aim of this study was to evaluate the effect of lysophosphatidic acid (LPA) on the follicular development, incidence of cell death, and expressions of apoptosis related genes and miR-22 in transplanted ovaries. Materials and Methods: In this experimental study, three-week-old mice ovaries were cultured for 24 hours in the presence and absence of LPA, and we assessed cell survival and normal follicular rates in some of the cultured ovaries. The remaining cultured ovaries were autotransplanted in the presence and absence of LPA as four experimental groups (LPA-/LPA-, LPA-/LPA+, LPA+/LPA-, LPA+/LPA+). The follicular development, immunohistochemistry for BAX, and expressions of genes related to apoptosis and miR-22 by real time reverse transcription polymerase chain reaction (RTPCR) were studied at the first oestrous cycles in the recovered ovaries. Sera 17-β-oestradiol (E2) and progesterone (P4) levels were also assessed. Results: Both cell survival and normal follicular rates were significantly higher in cultured ovaries in the presence of LPA after 24 hours (P<0.05). There was an increase in follicular development in comparison with the intact control group in the four transplanted groups (P<0.05). The LPA+/LPA- group had significantly higher follicular development, a decline in BAX positive cells, and a decrease in pro-apoptotic gene expressions in parallel with enhanced expression of anti-apoptotic and miR-22 genes and higher levels of hormones compared with the non-treated and intact control groups (P<0.05). Conclusion: LPA, as a survival factor, improves follicular development in transplanted ovaries by providing a balance between the anti- and pro-apoptotic genes in association with an increase in miR-22 expression.Maryam DehghanShirin ShahbaziMojdeh SalehniaRoyan Institute (ACECR), Tehranarticleapoptosisautotransplantationbax proteinlysophosphatidic acidovarian follicleMedicineRScienceQENCell Journal, Vol 23, Iss 5, Pp 584-592 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis autotransplantation bax protein lysophosphatidic acid ovarian follicle Medicine R Science Q
spellingShingle	apoptosis autotransplantation bax protein lysophosphatidic acid ovarian follicle Medicine R Science Q Maryam Dehghan Shirin Shahbazi Mojdeh Salehnia Lysophosphatidic Acid Alters The Expression of Apoptosis Related Genes and miR-22 in Cultured and Autotransplanted Ovaries
description	Objective: The aim of this study was to evaluate the effect of lysophosphatidic acid (LPA) on the follicular development, incidence of cell death, and expressions of apoptosis related genes and miR-22 in transplanted ovaries. Materials and Methods: In this experimental study, three-week-old mice ovaries were cultured for 24 hours in the presence and absence of LPA, and we assessed cell survival and normal follicular rates in some of the cultured ovaries. The remaining cultured ovaries were autotransplanted in the presence and absence of LPA as four experimental groups (LPA-/LPA-, LPA-/LPA+, LPA+/LPA-, LPA+/LPA+). The follicular development, immunohistochemistry for BAX, and expressions of genes related to apoptosis and miR-22 by real time reverse transcription polymerase chain reaction (RTPCR) were studied at the first oestrous cycles in the recovered ovaries. Sera 17-β-oestradiol (E2) and progesterone (P4) levels were also assessed. Results: Both cell survival and normal follicular rates were significantly higher in cultured ovaries in the presence of LPA after 24 hours (P<0.05). There was an increase in follicular development in comparison with the intact control group in the four transplanted groups (P<0.05). The LPA+/LPA- group had significantly higher follicular development, a decline in BAX positive cells, and a decrease in pro-apoptotic gene expressions in parallel with enhanced expression of anti-apoptotic and miR-22 genes and higher levels of hormones compared with the non-treated and intact control groups (P<0.05). Conclusion: LPA, as a survival factor, improves follicular development in transplanted ovaries by providing a balance between the anti- and pro-apoptotic genes in association with an increase in miR-22 expression.
format	article
author	Maryam Dehghan Shirin Shahbazi Mojdeh Salehnia
author_facet	Maryam Dehghan Shirin Shahbazi Mojdeh Salehnia
author_sort	Maryam Dehghan
title	Lysophosphatidic Acid Alters The Expression of Apoptosis Related Genes and miR-22 in Cultured and Autotransplanted Ovaries
title_short	Lysophosphatidic Acid Alters The Expression of Apoptosis Related Genes and miR-22 in Cultured and Autotransplanted Ovaries
title_full	Lysophosphatidic Acid Alters The Expression of Apoptosis Related Genes and miR-22 in Cultured and Autotransplanted Ovaries
title_fullStr	Lysophosphatidic Acid Alters The Expression of Apoptosis Related Genes and miR-22 in Cultured and Autotransplanted Ovaries
title_full_unstemmed	Lysophosphatidic Acid Alters The Expression of Apoptosis Related Genes and miR-22 in Cultured and Autotransplanted Ovaries
title_sort	lysophosphatidic acid alters the expression of apoptosis related genes and mir-22 in cultured and autotransplanted ovaries
publisher	Royan Institute (ACECR), Tehran
publishDate	2021
url	https://doaj.org/article/3cc7e31e3211490181c7cdc549ea5afc
work_keys_str_mv	AT maryamdehghan lysophosphatidicacidalterstheexpressionofapoptosisrelatedgenesandmir22inculturedandautotransplantedovaries AT shirinshahbazi lysophosphatidicacidalterstheexpressionofapoptosisrelatedgenesandmir22inculturedandautotransplantedovaries AT mojdehsalehnia lysophosphatidicacidalterstheexpressionofapoptosisrelatedgenesandmir22inculturedandautotransplantedovaries
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Lysophosphatidic Acid Alters The Expression of Apoptosis Related Genes and miR-22 in Cultured and Autotransplanted Ovaries

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