Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR

Rismaya, Azamris, Budiarti S, Desriani. 2017. Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR. Biodiversitas 18: 1041-1046. HER-2 amplification in breast cancer gives an implication in therapy and prognosis. FDA have been app...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	RISMAYA RISMAYA, AZAMRIS AZAMRIS, SRI BUDIARTI, DESRIANI DESRIANI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	breast cancer her-2 erbb2 qpcr fish ihc housekeeping gene Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3cc833c084764037abaf04d01622974b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3cc833c084764037abaf04d01622974b
record_format	dspace
spelling	oai:doaj.org-article:3cc833c084764037abaf04d01622974b2021-11-16T13:56:34ZHousekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR1412-033X2085-472210.13057/biodiv/d180323https://doaj.org/article/3cc833c084764037abaf04d01622974b2017-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2022https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Rismaya, Azamris, Budiarti S, Desriani. 2017. Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR. Biodiversitas 18: 1041-1046. HER-2 amplification in breast cancer gives an implication in therapy and prognosis. FDA have been approved Fluorescence In situ hybridization (FISH) for HER-2 amplification and immunohistochemistry (IHC) for protein expression measurement. In this recently times, quantitative PCR techniques have been reported as an alternative method for HER-2 amplification determination. Here in this report, we investigated new reference gene coming from housekeeping gene as an alternative calibrator for HER-2 amplification scoring based on qPCR methods. We compared two recommended housekeeping gene, 18S rRNA and ?-Actin, as a candidate for a HER-2 calibrator. For methods development, optimization and comparison study between 18S rRNA and ?-Actin, we used non-invasive samples (DNA buccal cells). Selected candidate reference further was tested to eighteen breast cancer sample, validated with qPCR methods refer to Mendoza et al. 2013. ?- Actin is shown as the best candidate for HER-2 scoring status determination. qPCR concordance result with other qPCR methods refers to Mendoza et al. 2013 shown 94.4%. The qPCR efficiency and % CV value shown as a requirement as a theory. qPCR efficiency was 103.8-105.3% for ?-Actin and HER-2 respectively while CV value was around 1.2%. Our result showed a significant correlation with reported methods, which could potentially complement with FDA approved methods.RISMAYA RISMAYAAZAMRIS AZAMRISSRI BUDIARTIDESRIANI DESRIANIMBI & UNS Soloarticlebreast cancerher-2erbb2qpcrfishihchousekeeping geneBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 3, Pp 1041-1046 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer her-2 erbb2 qpcr fish ihc housekeeping gene Biology (General) QH301-705.5
spellingShingle	breast cancer her-2 erbb2 qpcr fish ihc housekeeping gene Biology (General) QH301-705.5 RISMAYA RISMAYA AZAMRIS AZAMRIS SRI BUDIARTI DESRIANI DESRIANI Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR
description	Rismaya, Azamris, Budiarti S, Desriani. 2017. Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR. Biodiversitas 18: 1041-1046. HER-2 amplification in breast cancer gives an implication in therapy and prognosis. FDA have been approved Fluorescence In situ hybridization (FISH) for HER-2 amplification and immunohistochemistry (IHC) for protein expression measurement. In this recently times, quantitative PCR techniques have been reported as an alternative method for HER-2 amplification determination. Here in this report, we investigated new reference gene coming from housekeeping gene as an alternative calibrator for HER-2 amplification scoring based on qPCR methods. We compared two recommended housekeeping gene, 18S rRNA and ?-Actin, as a candidate for a HER-2 calibrator. For methods development, optimization and comparison study between 18S rRNA and ?-Actin, we used non-invasive samples (DNA buccal cells). Selected candidate reference further was tested to eighteen breast cancer sample, validated with qPCR methods refer to Mendoza et al. 2013. ?- Actin is shown as the best candidate for HER-2 scoring status determination. qPCR concordance result with other qPCR methods refers to Mendoza et al. 2013 shown 94.4%. The qPCR efficiency and % CV value shown as a requirement as a theory. qPCR efficiency was 103.8-105.3% for ?-Actin and HER-2 respectively while CV value was around 1.2%. Our result showed a significant correlation with reported methods, which could potentially complement with FDA approved methods.
format	article
author	RISMAYA RISMAYA AZAMRIS AZAMRIS SRI BUDIARTI DESRIANI DESRIANI
author_facet	RISMAYA RISMAYA AZAMRIS AZAMRIS SRI BUDIARTI DESRIANI DESRIANI
author_sort	RISMAYA RISMAYA
title	Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR
title_short	Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR
title_full	Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR
title_fullStr	Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR
title_full_unstemmed	Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR
title_sort	housekeeping gene as a source of calibrator candidate for her-2 scoring in frozen tissue breast cancer study based on qpcr
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/3cc833c084764037abaf04d01622974b
work_keys_str_mv	AT rismayarismaya housekeepinggeneasasourceofcalibratorcandidateforher2scoringinfrozentissuebreastcancerstudybasedonqpcr AT azamrisazamris housekeepinggeneasasourceofcalibratorcandidateforher2scoringinfrozentissuebreastcancerstudybasedonqpcr AT sribudiarti housekeepinggeneasasourceofcalibratorcandidateforher2scoringinfrozentissuebreastcancerstudybasedonqpcr AT desrianidesriani housekeepinggeneasasourceofcalibratorcandidateforher2scoringinfrozentissuebreastcancerstudybasedonqpcr
_version_	1718426475675254784

Housekeeping gene as a source of calibrator candidate for HER-2 scoring in frozen tissue breast cancer study based on qPCR

Ejemplares similares