Free radicals in the aqueous humor of patients with glaucoma

Eiki Oshida, Yukihiro Matsumoto, Kiyomi Arai1Department of Ophthalmology, Dokkyo Medical University Koshigaya Hospital, Koshigaya, Saitama, JapanPurpose: To clarify the presence of oxidative stress in glaucoma and discuss whether free radicals contribute to neovascular glaucoma (NVG) and non-NVG.Met...

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Autores principales:	Eiki Oshida, Yukihiro Matsumoto, Kiyomi Arai
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/3ccc48d428154112be96a4bbf133fce3
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spelling	oai:doaj.org-article:3ccc48d428154112be96a4bbf133fce32021-12-02T02:57:03ZFree radicals in the aqueous humor of patients with glaucoma1177-54671177-5483https://doaj.org/article/3ccc48d428154112be96a4bbf133fce32010-06-01T00:00:00Zhttp://www.dovepress.com/free-radicals-in-the-aqueous-humor-of-patients-with-glaucoma-a4636https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Eiki Oshida, Yukihiro Matsumoto, Kiyomi Arai1Department of Ophthalmology, Dokkyo Medical University Koshigaya Hospital, Koshigaya, Saitama, JapanPurpose: To clarify the presence of oxidative stress in glaucoma and discuss whether free radicals contribute to neovascular glaucoma (NVG) and non-NVG.Methods: Two patient groups were formed: the NVG group (n = 10) and the non-NVG group (n = 17). Free radicals in aqueous humor were detected by measuring the electron spin resonance (ESR). To identify free radical species, either superoxide dismutase (SOD) or catalase was added to the aqueous humor and compared with aqueous humor in which SOD or catalase was not added.Results: In the NVG group, free radical waveforms were detected that differed from ascorbate-free radical (AFR) in all cases. Under SOD was added, the characteristic waveforms disappeared and the AFR of a specific waveform appeared. After catalase was added, the waveforms were unchanged. In the non-NVG group, the AFR of specific waveforms were detected in all cases. In 4 cases, the waveforms detected the presence of a trace of superoxide.Conclusions: In the NVG group, superoxides were detected, suggesting that superoxide scavenging activity was decreased markedly. L-ascorbic acid likely has an antioxidative function in the non-NVG group, suggesting that the aqueous humor in the NVG group was under higher oxidative stress compared with the non-NVG group.Keywords: oxidative stress, free radicals, glaucoma, ascorbate Eiki OshidaYukihiro MatsumotoKiyomi AraiDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2010, Iss default, Pp 653-660 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Eiki Oshida Yukihiro Matsumoto Kiyomi Arai Free radicals in the aqueous humor of patients with glaucoma
description	Eiki Oshida, Yukihiro Matsumoto, Kiyomi Arai1Department of Ophthalmology, Dokkyo Medical University Koshigaya Hospital, Koshigaya, Saitama, JapanPurpose: To clarify the presence of oxidative stress in glaucoma and discuss whether free radicals contribute to neovascular glaucoma (NVG) and non-NVG.Methods: Two patient groups were formed: the NVG group (n = 10) and the non-NVG group (n = 17). Free radicals in aqueous humor were detected by measuring the electron spin resonance (ESR). To identify free radical species, either superoxide dismutase (SOD) or catalase was added to the aqueous humor and compared with aqueous humor in which SOD or catalase was not added.Results: In the NVG group, free radical waveforms were detected that differed from ascorbate-free radical (AFR) in all cases. Under SOD was added, the characteristic waveforms disappeared and the AFR of a specific waveform appeared. After catalase was added, the waveforms were unchanged. In the non-NVG group, the AFR of specific waveforms were detected in all cases. In 4 cases, the waveforms detected the presence of a trace of superoxide.Conclusions: In the NVG group, superoxides were detected, suggesting that superoxide scavenging activity was decreased markedly. L-ascorbic acid likely has an antioxidative function in the non-NVG group, suggesting that the aqueous humor in the NVG group was under higher oxidative stress compared with the non-NVG group.Keywords: oxidative stress, free radicals, glaucoma, ascorbate
format	article
author	Eiki Oshida Yukihiro Matsumoto Kiyomi Arai
author_facet	Eiki Oshida Yukihiro Matsumoto Kiyomi Arai
author_sort	Eiki Oshida
title	Free radicals in the aqueous humor of patients with glaucoma
title_short	Free radicals in the aqueous humor of patients with glaucoma
title_full	Free radicals in the aqueous humor of patients with glaucoma
title_fullStr	Free radicals in the aqueous humor of patients with glaucoma
title_full_unstemmed	Free radicals in the aqueous humor of patients with glaucoma
title_sort	free radicals in the aqueous humor of patients with glaucoma
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/3ccc48d428154112be96a4bbf133fce3
work_keys_str_mv	AT eikioshida freeradicalsintheaqueoushumorofpatientswithglaucoma AT yukihiromatsumoto freeradicalsintheaqueoushumorofpatientswithglaucoma AT kiyomiarai freeradicalsintheaqueoushumorofpatientswithglaucoma
_version_	1718402035573850112

Free radicals in the aqueous humor of patients with glaucoma

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