Concentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts

The objective of the study was to determine the concentration of BMP-15 and GDF-9 in the fluid of follicular cysts and ovarian follicles, and to compare their concentrations in mono- and polyoocyte follicles in gilts. The study involved two experiments conducted on the ovaries collected post-slaught...

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Autores principales:	Stankiewicz Tomasz, Błaszczyk Barbara
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2014
Materias:	ovarian cysts polyoocyte follicles bmp-15 gdf-9 elisa gilts Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/3cdbdce14ddf4cdcb40c60e800bc3551
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spelling	oai:doaj.org-article:3cdbdce14ddf4cdcb40c60e800bc35512021-11-17T21:27:52ZConcentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts1820-744810.2478/acve-2014-0003https://doaj.org/article/3cdbdce14ddf4cdcb40c60e800bc35512014-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2014-0003https://doaj.org/toc/1820-7448The objective of the study was to determine the concentration of BMP-15 and GDF-9 in the fluid of follicular cysts and ovarian follicles, and to compare their concentrations in mono- and polyoocyte follicles in gilts. The study involved two experiments conducted on the ovaries collected post-slaughter from gilts (7-8 months old). The first experiment covered 31 follicular single cyst gilts (15-25 mm in diameter) and 41 gilts without cysts. Follicular fluid from follicles of 8-10 mm in diameter (n=41) and 5-8 mm in diameter (n=41), and cystic fluid (n=31) were collected for analysis. The second experiment involved collecting follicular fluid from poly- (n=19) and monooocyte (n=22) follicles. The concentration of BMP-15 and GDF-9 was then determined in the samples using specimen-specific ELISA kits. The differences in the concentration of these factors were calculated by means of analysis of variance and a posthoc test. Duncan’s multiple range test was used to verify the significance of differences at P<0.05 and P<0.01. In addition, correlations between the factors were calculated. BMP-15 and GDF-9 levels in the cystic fluid were significantly higher than those in the follicular fluid (P<0.01). However, no differences were observed between various size follicles or between mono- and polyoocyte follicles. BMP-15 and GDF-9 concentrations were found to be positively correlated (P<0.01). Differences in BMP-15 and GDF-9 concentrations in ovarian follicles and follicular cysts, as evidenced by our study, indicate that these factors may be related to folliculogenesis disorders in gilts. What is more, the number of oocytes in ovarian follicles does not influence the intrafollicular concentration of BMP-15 and GDF-9.Stankiewicz TomaszBłaszczyk BarbaraSciendoarticleovarian cystspolyoocyte folliclesbmp-15gdf-9elisagiltsVeterinary medicineSF600-1100ENActa Veterinaria, Vol 64, Iss 1, Pp 24-32 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	ovarian cysts polyoocyte follicles bmp-15 gdf-9 elisa gilts Veterinary medicine SF600-1100
spellingShingle	ovarian cysts polyoocyte follicles bmp-15 gdf-9 elisa gilts Veterinary medicine SF600-1100 Stankiewicz Tomasz Błaszczyk Barbara Concentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts
description	The objective of the study was to determine the concentration of BMP-15 and GDF-9 in the fluid of follicular cysts and ovarian follicles, and to compare their concentrations in mono- and polyoocyte follicles in gilts. The study involved two experiments conducted on the ovaries collected post-slaughter from gilts (7-8 months old). The first experiment covered 31 follicular single cyst gilts (15-25 mm in diameter) and 41 gilts without cysts. Follicular fluid from follicles of 8-10 mm in diameter (n=41) and 5-8 mm in diameter (n=41), and cystic fluid (n=31) were collected for analysis. The second experiment involved collecting follicular fluid from poly- (n=19) and monooocyte (n=22) follicles. The concentration of BMP-15 and GDF-9 was then determined in the samples using specimen-specific ELISA kits. The differences in the concentration of these factors were calculated by means of analysis of variance and a posthoc test. Duncan’s multiple range test was used to verify the significance of differences at P<0.05 and P<0.01. In addition, correlations between the factors were calculated. BMP-15 and GDF-9 levels in the cystic fluid were significantly higher than those in the follicular fluid (P<0.01). However, no differences were observed between various size follicles or between mono- and polyoocyte follicles. BMP-15 and GDF-9 concentrations were found to be positively correlated (P<0.01). Differences in BMP-15 and GDF-9 concentrations in ovarian follicles and follicular cysts, as evidenced by our study, indicate that these factors may be related to folliculogenesis disorders in gilts. What is more, the number of oocytes in ovarian follicles does not influence the intrafollicular concentration of BMP-15 and GDF-9.
format	article
author	Stankiewicz Tomasz Błaszczyk Barbara
author_facet	Stankiewicz Tomasz Błaszczyk Barbara
author_sort	Stankiewicz Tomasz
title	Concentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts
title_short	Concentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts
title_full	Concentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts
title_fullStr	Concentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts
title_full_unstemmed	Concentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts
title_sort	concentrations of bone morphogenetic protein-15 (bmp-15) and growth differentiation factor-9 (gdf-9) in follicular cysts, mono - and polyoocyte follicles in gilts
publisher	Sciendo
publishDate	2014
url	https://doaj.org/article/3cdbdce14ddf4cdcb40c60e800bc3551
work_keys_str_mv	AT stankiewicztomasz concentrationsofbonemorphogeneticprotein15bmp15andgrowthdifferentiationfactor9gdf9infollicularcystsmonoandpolyoocytefolliclesingilts AT błaszczykbarbara concentrationsofbonemorphogeneticprotein15bmp15andgrowthdifferentiationfactor9gdf9infollicularcystsmonoandpolyoocytefolliclesingilts
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Concentrations of bone morphogenetic protein-15 (BMP-15) and growth differentiation factor-9 (GDF-9) in follicular cysts, mono - and polyoocyte follicles in gilts

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