Simultaneous Rapid Detection of Aflatoxin B<sub>1</sub> and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay
Spices are susceptible to contamination by aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) and ochratoxin A (OTA), which are both mycotoxins with high toxicity and carcinogenicity. In this study, we aimed to develop an immuno-chromatographic strip test for the simultaneous quantifi...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/3ce4cb2d6889458d89d6a24f9f8aad6d |
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| Sumario: | Spices are susceptible to contamination by aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) and ochratoxin A (OTA), which are both mycotoxins with high toxicity and carcinogenicity. In this study, we aimed to develop an immuno-chromatographic strip test for the simultaneous quantification of AFB<sub>1</sub> and OTA in spices by spraying the coupled antigens AFB<sub>1</sub>–ovalbumin (AFB<sub>1</sub>–OVA) and OTA–ovalbumin (OTA–OVA) on a nitrocellulose membrane. The test strip had high sensitivity, good specificity, and strong stability. The detection limits of these two mycotoxins in Chinese prickly ash, pepper, chili, cinnamon, and aniseed were 5 μg/kg. The false positivity rate was 2%, and the false negativity rate was 0%. The maximum coefficient of variation was 4.28% between batches and 5.72% within batches. The average recovery rates of AFB<sub>1</sub> and OTA in spices were 81.2–113.7% and 82.2–118.6%, respectively, and the relative standard deviation (RSD) was <10%. The actual sample detection was consistent with high performance liquid chromatography analysis results. Therefore, the immuno-chromatographic test strips developed in this study can be used for the on-site simultaneous detection of AFB<sub>1</sub> and OTA in spices. This method would allow the relevant regulatory agencies to strengthen supervision in an effort to reduce the possible human health hazards of such contaminated spices. |
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