Simultaneous Rapid Detection of Aflatoxin B<sub>1</sub> and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay

Simultaneous Rapid Detection of Aflatoxin B<sub>1</sub> and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay

Spices are susceptible to contamination by aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) and ochratoxin A (OTA), which are both mycotoxins with high toxicity and carcinogenicity. In this study, we aimed to develop an immuno-chromatographic strip test for the simultaneous quantifi...

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Autores principales: Xue Zhao, Xindi Jin, Zhang Lin, Qi Guo, Bin Liu, Yahong Yuan, Tianli Yue, Xubo Zhao
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
aflatoxin B<sub>1</sub>
ochratotoxin A
gold immuno-chromatography assay
spice
detection method
Chemical technology
TP1-1185
Acceso en línea:https://doaj.org/article/3ce4cb2d6889458d89d6a24f9f8aad6d
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spelling oai:doaj.org-article:3ce4cb2d6889458d89d6a24f9f8aad6d2021-11-25T17:34:58ZSimultaneous Rapid Detection of Aflatoxin B<sub>1</sub> and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay10.3390/foods101127382304-8158https://doaj.org/article/3ce4cb2d6889458d89d6a24f9f8aad6d2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2738https://doaj.org/toc/2304-8158Spices are susceptible to contamination by aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) and ochratoxin A (OTA), which are both mycotoxins with high toxicity and carcinogenicity. In this study, we aimed to develop an immuno-chromatographic strip test for the simultaneous quantification of AFB<sub>1</sub> and OTA in spices by spraying the coupled antigens AFB<sub>1</sub>–ovalbumin (AFB<sub>1</sub>–OVA) and OTA–ovalbumin (OTA–OVA) on a nitrocellulose membrane. The test strip had high sensitivity, good specificity, and strong stability. The detection limits of these two mycotoxins in Chinese prickly ash, pepper, chili, cinnamon, and aniseed were 5 μg/kg. The false positivity rate was 2%, and the false negativity rate was 0%. The maximum coefficient of variation was 4.28% between batches and 5.72% within batches. The average recovery rates of AFB<sub>1</sub> and OTA in spices were 81.2–113.7% and 82.2–118.6%, respectively, and the relative standard deviation (RSD) was <10%. The actual sample detection was consistent with high performance liquid chromatography analysis results. Therefore, the immuno-chromatographic test strips developed in this study can be used for the on-site simultaneous detection of AFB<sub>1</sub> and OTA in spices. This method would allow the relevant regulatory agencies to strengthen supervision in an effort to reduce the possible human health hazards of such contaminated spices.Xue ZhaoXindi JinZhang LinQi GuoBin LiuYahong YuanTianli YueXubo ZhaoMDPI AGarticleaflatoxin B<sub>1</sub>ochratotoxin Agold immuno-chromatography assayspicedetection methodChemical technologyTP1-1185ENFoods, Vol 10, Iss 2738, p 2738 (2021)
institution DOAJ
collection DOAJ
language EN
topic aflatoxin B<sub>1</sub>
ochratotoxin A
gold immuno-chromatography assay
spice
detection method
Chemical technology
TP1-1185
spellingShingle aflatoxin B<sub>1</sub>
ochratotoxin A
gold immuno-chromatography assay
spice
detection method
Chemical technology
TP1-1185
Xue Zhao
Xindi Jin
Zhang Lin
Qi Guo
Bin Liu
Yahong Yuan
Tianli Yue
Xubo Zhao
Simultaneous Rapid Detection of Aflatoxin B<sub>1</sub> and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay
description Spices are susceptible to contamination by aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) and ochratoxin A (OTA), which are both mycotoxins with high toxicity and carcinogenicity. In this study, we aimed to develop an immuno-chromatographic strip test for the simultaneous quantification of AFB<sub>1</sub> and OTA in spices by spraying the coupled antigens AFB<sub>1</sub>–ovalbumin (AFB<sub>1</sub>–OVA) and OTA–ovalbumin (OTA–OVA) on a nitrocellulose membrane. The test strip had high sensitivity, good specificity, and strong stability. The detection limits of these two mycotoxins in Chinese prickly ash, pepper, chili, cinnamon, and aniseed were 5 μg/kg. The false positivity rate was 2%, and the false negativity rate was 0%. The maximum coefficient of variation was 4.28% between batches and 5.72% within batches. The average recovery rates of AFB<sub>1</sub> and OTA in spices were 81.2–113.7% and 82.2–118.6%, respectively, and the relative standard deviation (RSD) was <10%. The actual sample detection was consistent with high performance liquid chromatography analysis results. Therefore, the immuno-chromatographic test strips developed in this study can be used for the on-site simultaneous detection of AFB<sub>1</sub> and OTA in spices. This method would allow the relevant regulatory agencies to strengthen supervision in an effort to reduce the possible human health hazards of such contaminated spices.
format article
author Xue Zhao
Xindi Jin
Zhang Lin
Qi Guo
Bin Liu
Yahong Yuan
Tianli Yue
Xubo Zhao
author_facet Xue Zhao
Xindi Jin
Zhang Lin
Qi Guo
Bin Liu
Yahong Yuan
Tianli Yue
Xubo Zhao
author_sort Xue Zhao
title Simultaneous Rapid Detection of Aflatoxin B<sub>1</sub> and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay
title_short Simultaneous Rapid Detection of Aflatoxin B<sub>1</sub> and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay
title_full Simultaneous Rapid Detection of Aflatoxin B<sub>1</sub> and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay
title_fullStr Simultaneous Rapid Detection of Aflatoxin B<sub>1</sub> and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay
title_full_unstemmed Simultaneous Rapid Detection of Aflatoxin B<sub>1</sub> and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay
title_sort simultaneous rapid detection of aflatoxin b<sub>1</sub> and ochratoxin a in spices using lateral flow immuno-chromatographic assay
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/3ce4cb2d6889458d89d6a24f9f8aad6d
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