Differential Expression of Hypothalamic and Gill-crh System With Osmotic Stress in the Euryhaline Black Porgy, Acanthopagrus schlegelii

The local gill production of corticotropin releasing hormone (crh) and crh-receptor (crhr) is hypothesized to play important roles during seawater (SW) and freshwater (FW) acclimation in euryhaline black porgy (Acanthopagrus schlegelii). The mRNA expression of crh, crhr, and Na+/K+-ATPase (a-nka) wa...

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Autores principales:	Adimoolam Aruna, Tsan-Ping Wang, Jyun-Cing Cao, Dan-Suei Lan, Ganesan Nagarajan, Ching-Fong Chang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	crh crhr α-nka gill fish brain Physiology QP1-981
Acceso en línea:	https://doaj.org/article/3ce952edd32f47c285a550c47056c54a
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spelling	oai:doaj.org-article:3ce952edd32f47c285a550c47056c54a2021-11-11T07:23:40ZDifferential Expression of Hypothalamic and Gill-crh System With Osmotic Stress in the Euryhaline Black Porgy, Acanthopagrus schlegelii1664-042X10.3389/fphys.2021.768122https://doaj.org/article/3ce952edd32f47c285a550c47056c54a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.768122/fullhttps://doaj.org/toc/1664-042XThe local gill production of corticotropin releasing hormone (crh) and crh-receptor (crhr) is hypothesized to play important roles during seawater (SW) and freshwater (FW) acclimation in euryhaline black porgy (Acanthopagrus schlegelii). The mRNA expression of crh, crhr, and Na+/K+-ATPase (a-nka) was examined in SW and FW diencephalon (Dien) and in the gills at different exposure time by Q-PCR analysis. The in situ hybridization results indicate that crh mRNA hybridization signals were more abundant in FW fish in the gigantocellular (PMgc) and parvocellular (PMpc) part of the magnocellular preoptic nucleus versus SW fish. The crh and crhr-expressing cells were located in basal cells of gill filament. Furthermore, in vitro dexamethasone (DEX) treatment could increase the crh-system in the gill. Increased transcripts of the crh-system in the gill via in vitro and in vivo CRH treatments suggest that CRH may regulate the system in a local manner. The a-Nka cells were localized in the filament and secondary lamellae mitochondria rich cells (MRCs) of FW fish at 8 h and 1 day. a-Nka cells were seen in both filament and lamellae in the FW but much less in SW fish indicating that gills play key roles in black porgy osmoregulation. Gill crh and crhr play important roles in the response to salinity stress.Adimoolam ArunaTsan-Ping WangJyun-Cing CaoDan-Suei LanGanesan NagarajanGanesan NagarajanChing-Fong ChangChing-Fong ChangFrontiers Media S.A.articlecrhcrhrα-nkagillfishbrainPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	crh crhr α-nka gill fish brain Physiology QP1-981
spellingShingle	crh crhr α-nka gill fish brain Physiology QP1-981 Adimoolam Aruna Tsan-Ping Wang Jyun-Cing Cao Dan-Suei Lan Ganesan Nagarajan Ganesan Nagarajan Ching-Fong Chang Ching-Fong Chang Differential Expression of Hypothalamic and Gill-crh System With Osmotic Stress in the Euryhaline Black Porgy, Acanthopagrus schlegelii
description	The local gill production of corticotropin releasing hormone (crh) and crh-receptor (crhr) is hypothesized to play important roles during seawater (SW) and freshwater (FW) acclimation in euryhaline black porgy (Acanthopagrus schlegelii). The mRNA expression of crh, crhr, and Na+/K+-ATPase (a-nka) was examined in SW and FW diencephalon (Dien) and in the gills at different exposure time by Q-PCR analysis. The in situ hybridization results indicate that crh mRNA hybridization signals were more abundant in FW fish in the gigantocellular (PMgc) and parvocellular (PMpc) part of the magnocellular preoptic nucleus versus SW fish. The crh and crhr-expressing cells were located in basal cells of gill filament. Furthermore, in vitro dexamethasone (DEX) treatment could increase the crh-system in the gill. Increased transcripts of the crh-system in the gill via in vitro and in vivo CRH treatments suggest that CRH may regulate the system in a local manner. The a-Nka cells were localized in the filament and secondary lamellae mitochondria rich cells (MRCs) of FW fish at 8 h and 1 day. a-Nka cells were seen in both filament and lamellae in the FW but much less in SW fish indicating that gills play key roles in black porgy osmoregulation. Gill crh and crhr play important roles in the response to salinity stress.
format	article
author	Adimoolam Aruna Tsan-Ping Wang Jyun-Cing Cao Dan-Suei Lan Ganesan Nagarajan Ganesan Nagarajan Ching-Fong Chang Ching-Fong Chang
author_facet	Adimoolam Aruna Tsan-Ping Wang Jyun-Cing Cao Dan-Suei Lan Ganesan Nagarajan Ganesan Nagarajan Ching-Fong Chang Ching-Fong Chang
author_sort	Adimoolam Aruna
title	Differential Expression of Hypothalamic and Gill-crh System With Osmotic Stress in the Euryhaline Black Porgy, Acanthopagrus schlegelii
title_short	Differential Expression of Hypothalamic and Gill-crh System With Osmotic Stress in the Euryhaline Black Porgy, Acanthopagrus schlegelii
title_full	Differential Expression of Hypothalamic and Gill-crh System With Osmotic Stress in the Euryhaline Black Porgy, Acanthopagrus schlegelii
title_fullStr	Differential Expression of Hypothalamic and Gill-crh System With Osmotic Stress in the Euryhaline Black Porgy, Acanthopagrus schlegelii
title_full_unstemmed	Differential Expression of Hypothalamic and Gill-crh System With Osmotic Stress in the Euryhaline Black Porgy, Acanthopagrus schlegelii
title_sort	differential expression of hypothalamic and gill-crh system with osmotic stress in the euryhaline black porgy, acanthopagrus schlegelii
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3ce952edd32f47c285a550c47056c54a
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Differential Expression of Hypothalamic and Gill-crh System With Osmotic Stress in the Euryhaline Black Porgy, Acanthopagrus schlegelii

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