α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility

Abstract The balance between ovarian folliculogenesis and follicular atresia is critical for female fertility and is strictly regulated by a complex network of neuroendocrine and intra-ovarian signals. Despite the numerous functions executed by granulosa cells (GCs) in ovarian physiology, the role o...

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Autores principales:	Alexis Arcos, Matilde de Paola, Diego Gianetti, Diego Acuña, Zahady D. Velásquez, María Paz Miró, Gabriela Toro, Bryan Hinrichsen, Rosa Iris Muñoz, Yimo Lin, Gonzalo A. Mardones, Pamela Ehrenfeld, Francisco J. Rivera, Marcela A. Michaut, Luis Federico Batiz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ceea81d511e4c4e9bf27c8800f69b4e
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spelling	oai:doaj.org-article:3ceea81d511e4c4e9bf27c8800f69b4e2021-12-02T15:05:42Zα-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility10.1038/s41598-017-12292-92045-2322https://doaj.org/article/3ceea81d511e4c4e9bf27c8800f69b4e2017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-12292-9https://doaj.org/toc/2045-2322Abstract The balance between ovarian folliculogenesis and follicular atresia is critical for female fertility and is strictly regulated by a complex network of neuroendocrine and intra-ovarian signals. Despite the numerous functions executed by granulosa cells (GCs) in ovarian physiology, the role of multifunctional proteins able to simultaneously coordinate/modulate several cellular pathways is unclear. Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (α-SNAP) is a multifunctional protein that participates in SNARE-mediated membrane fusion events. In addition, it regulates cell-to-cell adhesion, AMPK signaling, autophagy and apoptosis in different cell types. In this study we examined the expression pattern of α-SNAP in ovarian tissue and the consequences of α-SNAP (M105I) mutation (hyh mutation) in folliculogenesis and female fertility. Our results showed that α-SNAP protein is highly expressed in GCs and its expression is modulated by gonadotropin stimuli. On the other hand, α-SNAP-mutant mice show a reduction in α-SNAP protein levels. Moreover, increased apoptosis of GCs and follicular atresia, reduced ovulation rate, and a dramatic decline in fertility is observed in α-SNAP-mutant females. In conclusion, α-SNAP plays a critical role in the balance between follicular development and atresia. Consequently, a reduction in its expression/function (M105I mutation) causes early depletion of ovarian follicles and female subfertility.Alexis ArcosMatilde de PaolaDiego GianettiDiego AcuñaZahady D. VelásquezMaría Paz MiróGabriela ToroBryan HinrichsenRosa Iris MuñozYimo LinGonzalo A. MardonesPamela EhrenfeldFrancisco J. RiveraMarcela A. MichautLuis Federico BatizNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexis Arcos Matilde de Paola Diego Gianetti Diego Acuña Zahady D. Velásquez María Paz Miró Gabriela Toro Bryan Hinrichsen Rosa Iris Muñoz Yimo Lin Gonzalo A. Mardones Pamela Ehrenfeld Francisco J. Rivera Marcela A. Michaut Luis Federico Batiz α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
description	Abstract The balance between ovarian folliculogenesis and follicular atresia is critical for female fertility and is strictly regulated by a complex network of neuroendocrine and intra-ovarian signals. Despite the numerous functions executed by granulosa cells (GCs) in ovarian physiology, the role of multifunctional proteins able to simultaneously coordinate/modulate several cellular pathways is unclear. Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (α-SNAP) is a multifunctional protein that participates in SNARE-mediated membrane fusion events. In addition, it regulates cell-to-cell adhesion, AMPK signaling, autophagy and apoptosis in different cell types. In this study we examined the expression pattern of α-SNAP in ovarian tissue and the consequences of α-SNAP (M105I) mutation (hyh mutation) in folliculogenesis and female fertility. Our results showed that α-SNAP protein is highly expressed in GCs and its expression is modulated by gonadotropin stimuli. On the other hand, α-SNAP-mutant mice show a reduction in α-SNAP protein levels. Moreover, increased apoptosis of GCs and follicular atresia, reduced ovulation rate, and a dramatic decline in fertility is observed in α-SNAP-mutant females. In conclusion, α-SNAP plays a critical role in the balance between follicular development and atresia. Consequently, a reduction in its expression/function (M105I mutation) causes early depletion of ovarian follicles and female subfertility.
format	article
author	Alexis Arcos Matilde de Paola Diego Gianetti Diego Acuña Zahady D. Velásquez María Paz Miró Gabriela Toro Bryan Hinrichsen Rosa Iris Muñoz Yimo Lin Gonzalo A. Mardones Pamela Ehrenfeld Francisco J. Rivera Marcela A. Michaut Luis Federico Batiz
author_facet	Alexis Arcos Matilde de Paola Diego Gianetti Diego Acuña Zahady D. Velásquez María Paz Miró Gabriela Toro Bryan Hinrichsen Rosa Iris Muñoz Yimo Lin Gonzalo A. Mardones Pamela Ehrenfeld Francisco J. Rivera Marcela A. Michaut Luis Federico Batiz
author_sort	Alexis Arcos
title	α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
title_short	α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
title_full	α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
title_fullStr	α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
title_full_unstemmed	α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
title_sort	α-snap is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3ceea81d511e4c4e9bf27c8800f69b4e
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α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility

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