ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma

Background. ANLN and miR-30a-5p may be involved in the progression of lung adenocarcinoma (LUAD). However, their underlying mechanism in LUAD has not been completely comprehended. Methods. Differential expression analysis, binding site prediction, and survival analysis were conducted by bioinformati...

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Autores principales:	Feng Deng, Zhili Xu, Jun Zhou, Ruhu Zhang, Xiaowei Gong
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Computer applications to medicine. Medical informatics R858-859.7
Acceso en línea:	https://doaj.org/article/3cfb6eb1edae4dc5807130728bd6a6df
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spelling	oai:doaj.org-article:3cfb6eb1edae4dc5807130728bd6a6df2021-11-15T01:19:23ZANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma1748-671810.1155/2021/9549287https://doaj.org/article/3cfb6eb1edae4dc5807130728bd6a6df2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9549287https://doaj.org/toc/1748-6718Background. ANLN and miR-30a-5p may be involved in the progression of lung adenocarcinoma (LUAD). However, their underlying mechanism in LUAD has not been completely comprehended. Methods. Differential expression analysis, binding site prediction, and survival analysis were conducted by bioinformatics approaches. ANLN mRNA and miR-30a-5p expression were detected by qRT-PCR. ANLN protein expression was detected by western blot. Cell behaviors in LUAD were examined by functional experiments. Results. ANLN was activated in LUAD cells in terms of mRNA and protein. High ANLN level was positively correlated with poor prognosis. Enforced ANLN stimulated protumorigenesis LUAD cell behaviors. miR-30a-5p could target ANLN mRNA, as revealed and verified through assays. Remarkably low miR-30a-5p expression was observed in LUAD cells, and it could repress ANLN expression. The accelerated cell behaviors by overexpression of ANLN were counteracted by upregulating miR-30a-5p. Conclusion. Overall, miR-30a-5p remarkably restrained the malignant progression of LUAD cells by constraining ANLN expression. Thus, ANLN and miR-30a-5p could be novel therapeutic targets of LUAD.Feng DengZhili XuJun ZhouRuhu ZhangXiaowei GongHindawi LimitedarticleComputer applications to medicine. Medical informaticsR858-859.7ENComputational and Mathematical Methods in Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Computer applications to medicine. Medical informatics R858-859.7
spellingShingle	Computer applications to medicine. Medical informatics R858-859.7 Feng Deng Zhili Xu Jun Zhou Ruhu Zhang Xiaowei Gong ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma
description	Background. ANLN and miR-30a-5p may be involved in the progression of lung adenocarcinoma (LUAD). However, their underlying mechanism in LUAD has not been completely comprehended. Methods. Differential expression analysis, binding site prediction, and survival analysis were conducted by bioinformatics approaches. ANLN mRNA and miR-30a-5p expression were detected by qRT-PCR. ANLN protein expression was detected by western blot. Cell behaviors in LUAD were examined by functional experiments. Results. ANLN was activated in LUAD cells in terms of mRNA and protein. High ANLN level was positively correlated with poor prognosis. Enforced ANLN stimulated protumorigenesis LUAD cell behaviors. miR-30a-5p could target ANLN mRNA, as revealed and verified through assays. Remarkably low miR-30a-5p expression was observed in LUAD cells, and it could repress ANLN expression. The accelerated cell behaviors by overexpression of ANLN were counteracted by upregulating miR-30a-5p. Conclusion. Overall, miR-30a-5p remarkably restrained the malignant progression of LUAD cells by constraining ANLN expression. Thus, ANLN and miR-30a-5p could be novel therapeutic targets of LUAD.
format	article
author	Feng Deng Zhili Xu Jun Zhou Ruhu Zhang Xiaowei Gong
author_facet	Feng Deng Zhili Xu Jun Zhou Ruhu Zhang Xiaowei Gong
author_sort	Feng Deng
title	ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma
title_short	ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma
title_full	ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma
title_fullStr	ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma
title_full_unstemmed	ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma
title_sort	anln regulated by mir-30a-5p mediates malignant progression of lung adenocarcinoma
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/3cfb6eb1edae4dc5807130728bd6a6df
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ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma

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