Moderate Beer Intake Downregulates Inflammasome Pathway Gene Expression in Human Macrophages

Inflammasomes are key components of the innate immunity system that trigger the inflammatory response. Inappropriate activity of the inflammasome system has been linked to onset and perpetuation of inflammation in atherosclerotic plaques and cardiovascular disease. Low-to-moderate beer consumption i...

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Autores principales:	Natàlia Muñoz-Garcia, Rafael Escate, Lina Badimon, Teresa Padro
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	inflammasome beer macrophages atherosclerosis gene expression fermented beverages Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3cff9968876c42409c254953383d1693
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spelling	oai:doaj.org-article:3cff9968876c42409c254953383d16932021-11-25T16:47:33ZModerate Beer Intake Downregulates Inflammasome Pathway Gene Expression in Human Macrophages10.3390/biology101111592079-7737https://doaj.org/article/3cff9968876c42409c254953383d16932021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1159https://doaj.org/toc/2079-7737Inflammasomes are key components of the innate immunity system that trigger the inflammatory response. Inappropriate activity of the inflammasome system has been linked to onset and perpetuation of inflammation in atherosclerotic plaques and cardiovascular disease. Low-to-moderate beer consumption is inversely associated with cardiovascular event presentation, while high levels of alcohol intake are associated with increased cardiovascular risk. Although fermented beverages have been suggested to exert their beneficial effects through their anti-oxidant and anti-inflammatory properties, little is known regarding the capacity of beer to modulate innate immunity cell responses. To this aim, primed or activated THP-1 macrophages were conditioned with human serum obtained from a prospective two-arms longitudinal crossover study to investigate the effect of a moderate and regular daily intake of beer, either alcohol-free or traditional, in the regulation of TLR-mediated inflammatory responses in healthy but overweight individuals. Conditioned macrophages with serum obtained after four-week intervention with alcohol-free beer significantly reduced the transcription of pro-inflammatory interleukins such as IL-1β and TNF. The serum of traditional beer consumers did not exhibit the same capacity as the serum of alcohol-free beer consumers to reduce gene expression of pro-inflammatory interleukins; however, serum from traditional beer consumers showed a regulatory effect at the protein level by significantly decreasing the intracellular protein levels of pro-IL-1β in primed macrophages and preventing cleaved-IL-1β protein release.Natàlia Muñoz-GarciaRafael EscateLina BadimonTeresa PadroMDPI AGarticleinflammasomebeermacrophagesatherosclerosisgene expressionfermented beveragesBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1159, p 1159 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	inflammasome beer macrophages atherosclerosis gene expression fermented beverages Biology (General) QH301-705.5
spellingShingle	inflammasome beer macrophages atherosclerosis gene expression fermented beverages Biology (General) QH301-705.5 Natàlia Muñoz-Garcia Rafael Escate Lina Badimon Teresa Padro Moderate Beer Intake Downregulates Inflammasome Pathway Gene Expression in Human Macrophages
description	Inflammasomes are key components of the innate immunity system that trigger the inflammatory response. Inappropriate activity of the inflammasome system has been linked to onset and perpetuation of inflammation in atherosclerotic plaques and cardiovascular disease. Low-to-moderate beer consumption is inversely associated with cardiovascular event presentation, while high levels of alcohol intake are associated with increased cardiovascular risk. Although fermented beverages have been suggested to exert their beneficial effects through their anti-oxidant and anti-inflammatory properties, little is known regarding the capacity of beer to modulate innate immunity cell responses. To this aim, primed or activated THP-1 macrophages were conditioned with human serum obtained from a prospective two-arms longitudinal crossover study to investigate the effect of a moderate and regular daily intake of beer, either alcohol-free or traditional, in the regulation of TLR-mediated inflammatory responses in healthy but overweight individuals. Conditioned macrophages with serum obtained after four-week intervention with alcohol-free beer significantly reduced the transcription of pro-inflammatory interleukins such as IL-1β and TNF. The serum of traditional beer consumers did not exhibit the same capacity as the serum of alcohol-free beer consumers to reduce gene expression of pro-inflammatory interleukins; however, serum from traditional beer consumers showed a regulatory effect at the protein level by significantly decreasing the intracellular protein levels of pro-IL-1β in primed macrophages and preventing cleaved-IL-1β protein release.
format	article
author	Natàlia Muñoz-Garcia Rafael Escate Lina Badimon Teresa Padro
author_facet	Natàlia Muñoz-Garcia Rafael Escate Lina Badimon Teresa Padro
author_sort	Natàlia Muñoz-Garcia
title	Moderate Beer Intake Downregulates Inflammasome Pathway Gene Expression in Human Macrophages
title_short	Moderate Beer Intake Downregulates Inflammasome Pathway Gene Expression in Human Macrophages
title_full	Moderate Beer Intake Downregulates Inflammasome Pathway Gene Expression in Human Macrophages
title_fullStr	Moderate Beer Intake Downregulates Inflammasome Pathway Gene Expression in Human Macrophages
title_full_unstemmed	Moderate Beer Intake Downregulates Inflammasome Pathway Gene Expression in Human Macrophages
title_sort	moderate beer intake downregulates inflammasome pathway gene expression in human macrophages
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3cff9968876c42409c254953383d1693
work_keys_str_mv	AT nataliamunozgarcia moderatebeerintakedownregulatesinflammasomepathwaygeneexpressioninhumanmacrophages AT rafaelescate moderatebeerintakedownregulatesinflammasomepathwaygeneexpressioninhumanmacrophages AT linabadimon moderatebeerintakedownregulatesinflammasomepathwaygeneexpressioninhumanmacrophages AT teresapadro moderatebeerintakedownregulatesinflammasomepathwaygeneexpressioninhumanmacrophages
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Moderate Beer Intake Downregulates Inflammasome Pathway Gene Expression in Human Macrophages

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