Probiotics into outer space: feasibility assessments of encapsulated freeze-dried probiotics during 1 month’s storage on the International Space Station
Abstract Suppression of immune function during long spaceflights is an issue that needs to be overcome. The well-established probiotic Lactobacillus casei strain Shirota (LcS) could be a promising countermeasure, and we have launched a project to investigate the efficacy of its use on the Internatio...
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2018
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/3d033919f06f43bf9266eb40c71a05fa |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:3d033919f06f43bf9266eb40c71a05fa |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:3d033919f06f43bf9266eb40c71a05fa2021-12-02T15:09:09ZProbiotics into outer space: feasibility assessments of encapsulated freeze-dried probiotics during 1 month’s storage on the International Space Station10.1038/s41598-018-29094-22045-2322https://doaj.org/article/3d033919f06f43bf9266eb40c71a05fa2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29094-2https://doaj.org/toc/2045-2322Abstract Suppression of immune function during long spaceflights is an issue that needs to be overcome. The well-established probiotic Lactobacillus casei strain Shirota (LcS) could be a promising countermeasure, and we have launched a project to investigate the efficacy of its use on the International Space Station (ISS). As a first step, we developed a specialist probiotic product for space experiments, containing freeze-dried LcS in capsule form (Probiotics Package), and tested its stability through 1 month of storage on the ISS. The temperature inside the ISS ranged from 20.0 to 24.5 °C. The absorbed dose rate of the flight sample was 0.26 mGy/day and the dose equivalent rate was 0.52 mSv/day. The number of live LcS was 1.05 × 1011 colony-forming units/g powder (49.5% of the initial value) 6 months after the start of the study; this value was comparable to those in the two ground controls. Profiles of randomly amplified polymorphic DNA, sequence variant frequency, carbohydrate fermentation, reactivity to LcS-specific antibody, and the cytokine-inducing ability of LcS in the flight sample did not differ from those of the ground controls. We can therefore maintain the viability and basic probiotic properties of LcS stored as a Probiotics Package on the ISS.Takafumi SakaiYasuhiro MotekiTakuya TakahashiKan ShidaMayumi KiwakiYasuhisa ShimakawaAkihisa MatsuiOsamu ChonanKazuya MorikawaToshiko OhtaHiroshi OhshimaSatoshi FurukawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Takafumi Sakai Yasuhiro Moteki Takuya Takahashi Kan Shida Mayumi Kiwaki Yasuhisa Shimakawa Akihisa Matsui Osamu Chonan Kazuya Morikawa Toshiko Ohta Hiroshi Ohshima Satoshi Furukawa Probiotics into outer space: feasibility assessments of encapsulated freeze-dried probiotics during 1 month’s storage on the International Space Station |
| description |
Abstract Suppression of immune function during long spaceflights is an issue that needs to be overcome. The well-established probiotic Lactobacillus casei strain Shirota (LcS) could be a promising countermeasure, and we have launched a project to investigate the efficacy of its use on the International Space Station (ISS). As a first step, we developed a specialist probiotic product for space experiments, containing freeze-dried LcS in capsule form (Probiotics Package), and tested its stability through 1 month of storage on the ISS. The temperature inside the ISS ranged from 20.0 to 24.5 °C. The absorbed dose rate of the flight sample was 0.26 mGy/day and the dose equivalent rate was 0.52 mSv/day. The number of live LcS was 1.05 × 1011 colony-forming units/g powder (49.5% of the initial value) 6 months after the start of the study; this value was comparable to those in the two ground controls. Profiles of randomly amplified polymorphic DNA, sequence variant frequency, carbohydrate fermentation, reactivity to LcS-specific antibody, and the cytokine-inducing ability of LcS in the flight sample did not differ from those of the ground controls. We can therefore maintain the viability and basic probiotic properties of LcS stored as a Probiotics Package on the ISS. |
| format |
article |
| author |
Takafumi Sakai Yasuhiro Moteki Takuya Takahashi Kan Shida Mayumi Kiwaki Yasuhisa Shimakawa Akihisa Matsui Osamu Chonan Kazuya Morikawa Toshiko Ohta Hiroshi Ohshima Satoshi Furukawa |
| author_facet |
Takafumi Sakai Yasuhiro Moteki Takuya Takahashi Kan Shida Mayumi Kiwaki Yasuhisa Shimakawa Akihisa Matsui Osamu Chonan Kazuya Morikawa Toshiko Ohta Hiroshi Ohshima Satoshi Furukawa |
| author_sort |
Takafumi Sakai |
| title |
Probiotics into outer space: feasibility assessments of encapsulated freeze-dried probiotics during 1 month’s storage on the International Space Station |
| title_short |
Probiotics into outer space: feasibility assessments of encapsulated freeze-dried probiotics during 1 month’s storage on the International Space Station |
| title_full |
Probiotics into outer space: feasibility assessments of encapsulated freeze-dried probiotics during 1 month’s storage on the International Space Station |
| title_fullStr |
Probiotics into outer space: feasibility assessments of encapsulated freeze-dried probiotics during 1 month’s storage on the International Space Station |
| title_full_unstemmed |
Probiotics into outer space: feasibility assessments of encapsulated freeze-dried probiotics during 1 month’s storage on the International Space Station |
| title_sort |
probiotics into outer space: feasibility assessments of encapsulated freeze-dried probiotics during 1 month’s storage on the international space station |
| publisher |
Nature Portfolio |
| publishDate |
2018 |
| url |
https://doaj.org/article/3d033919f06f43bf9266eb40c71a05fa |
| work_keys_str_mv |
AT takafumisakai probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT yasuhiromoteki probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT takuyatakahashi probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT kanshida probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT mayumikiwaki probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT yasuhisashimakawa probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT akihisamatsui probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT osamuchonan probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT kazuyamorikawa probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT toshikoohta probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT hiroshiohshima probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation AT satoshifurukawa probioticsintoouterspacefeasibilityassessmentsofencapsulatedfreezedriedprobioticsduring1monthsstorageontheinternationalspacestation |
| _version_ |
1718387867982495744 |