Stac3 is a novel regulator of skeletal muscle development in mice.

The goal of this study was to identify novel factors that mediate skeletal muscle development or function. We began the study by searching the gene expression databases for genes that have no known functions but are preferentially expressed in skeletal muscle. This search led to the identification o...

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Autores principales:	Brad M Reinholt, Xiaomei Ge, Xiaofei Cong, David E Gerrard, Honglin Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3d05cb39958e485286d5caebb988c376
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spelling	oai:doaj.org-article:3d05cb39958e485286d5caebb988c3762021-11-18T07:48:13ZStac3 is a novel regulator of skeletal muscle development in mice.1932-620310.1371/journal.pone.0062760https://doaj.org/article/3d05cb39958e485286d5caebb988c3762013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23626854/?tool=EBIhttps://doaj.org/toc/1932-6203The goal of this study was to identify novel factors that mediate skeletal muscle development or function. We began the study by searching the gene expression databases for genes that have no known functions but are preferentially expressed in skeletal muscle. This search led to the identification of the Src homology three (SH3) domain and cysteine rich (C1) domain 3 (Stac3) gene. We experimentally confirmed that Stac3 mRNA was predominantly expressed in skeletal muscle. We determined if Stac3 plays a role in skeletal muscle development or function by generating Stac3 knockout mice. All Stac3 homozygous mutant mice were found dead at birth, were never seen move, and had a curved body and dropping forelimbs. These mice had marked abnormalities in skeletal muscles throughout the body, including central location of myonuclei, decreased number but increased cross-sectional area of myofibers, decreased number and size of myofibrils, disarrayed myofibrils, and streaming Z-lines. These phenotypes demonstrate that the Stac3 gene plays a critical role in skeletal muscle development and function in mice.Brad M ReinholtXiaomei GeXiaofei CongDavid E GerrardHonglin JiangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62760 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brad M Reinholt Xiaomei Ge Xiaofei Cong David E Gerrard Honglin Jiang Stac3 is a novel regulator of skeletal muscle development in mice.
description	The goal of this study was to identify novel factors that mediate skeletal muscle development or function. We began the study by searching the gene expression databases for genes that have no known functions but are preferentially expressed in skeletal muscle. This search led to the identification of the Src homology three (SH3) domain and cysteine rich (C1) domain 3 (Stac3) gene. We experimentally confirmed that Stac3 mRNA was predominantly expressed in skeletal muscle. We determined if Stac3 plays a role in skeletal muscle development or function by generating Stac3 knockout mice. All Stac3 homozygous mutant mice were found dead at birth, were never seen move, and had a curved body and dropping forelimbs. These mice had marked abnormalities in skeletal muscles throughout the body, including central location of myonuclei, decreased number but increased cross-sectional area of myofibers, decreased number and size of myofibrils, disarrayed myofibrils, and streaming Z-lines. These phenotypes demonstrate that the Stac3 gene plays a critical role in skeletal muscle development and function in mice.
format	article
author	Brad M Reinholt Xiaomei Ge Xiaofei Cong David E Gerrard Honglin Jiang
author_facet	Brad M Reinholt Xiaomei Ge Xiaofei Cong David E Gerrard Honglin Jiang
author_sort	Brad M Reinholt
title	Stac3 is a novel regulator of skeletal muscle development in mice.
title_short	Stac3 is a novel regulator of skeletal muscle development in mice.
title_full	Stac3 is a novel regulator of skeletal muscle development in mice.
title_fullStr	Stac3 is a novel regulator of skeletal muscle development in mice.
title_full_unstemmed	Stac3 is a novel regulator of skeletal muscle development in mice.
title_sort	stac3 is a novel regulator of skeletal muscle development in mice.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3d05cb39958e485286d5caebb988c376
work_keys_str_mv	AT bradmreinholt stac3isanovelregulatorofskeletalmuscledevelopmentinmice AT xiaomeige stac3isanovelregulatorofskeletalmuscledevelopmentinmice AT xiaofeicong stac3isanovelregulatorofskeletalmuscledevelopmentinmice AT davidegerrard stac3isanovelregulatorofskeletalmuscledevelopmentinmice AT honglinjiang stac3isanovelregulatorofskeletalmuscledevelopmentinmice
_version_	1718422905595887616

Stac3 is a novel regulator of skeletal muscle development in mice.

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