Isolation and characterization marine bacteria capable of degrading lignin-derived compounds.

Lignin, a characteristic component of terrestrial plants. Rivers transport large amounts of vascular plant organic matter into the oceans where lignin can degrade over time; however, microorganisms involved in this degradation have not been identified. In this study, several bacterial strains were i...

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Autores principales:	Peng Lu, Weinan Wang, Guangxi Zhang, Wen Li, Anjie Jiang, Mengjiao Cao, Xiaoyan Zhang, Ke Xing, Xue Peng, Bo Yuan, Zhaozhong Feng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3d0692cf38cc4a68b85b0aaf0d9ca3f8
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spelling	oai:doaj.org-article:3d0692cf38cc4a68b85b0aaf0d9ca3f82021-12-02T20:05:46ZIsolation and characterization marine bacteria capable of degrading lignin-derived compounds.1932-620310.1371/journal.pone.0240187https://doaj.org/article/3d0692cf38cc4a68b85b0aaf0d9ca3f82020-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0240187https://doaj.org/toc/1932-6203Lignin, a characteristic component of terrestrial plants. Rivers transport large amounts of vascular plant organic matter into the oceans where lignin can degrade over time; however, microorganisms involved in this degradation have not been identified. In this study, several bacterial strains were isolated from marine samples using the lignin-derived compound vanillic acid (4-hydroxy-3-methoxybenzoic acid) as the sole carbon and energy source. The optimum growth temperature for all isolates ranged from 30 to 35°C. All isolates grew well in a wide NaCl concentration range of 0 to over 50 g/L, with an optimum concentration of 22.8 g/L, which is the same as natural seawater. Phylogenetic analysis indicates that these strains are the members of Halomonas, Arthrobacter, Pseudoalteromonas, Marinomonas, and Thalassospira. These isolates are also able to use other lignin-derived compounds, such as 4-hydroxybenzoic acid, ferulic acid, syringic acid, and benzoic acid. Vanillic acid was detected in all culture media when isolates were grown on ferulic acid as the sole carbon source; however, no 4-hydroxy-3-methoxystyrene was detected, indicating that ferulic acid metabolism by these strains occurs via the elimination of two side chain carbons. Furthermore, the isolates exhibit 3,4-dioxygenase or 4,5-dioxygenase activity for protocatechuic acid ring-cleavage, which is consistent with the genetic sequences of related genera. This study was conducted to isolate and characterize marine bacteria of degrading lignin-derived compounds, thereby revealing the degradation of aromatic compounds in the marine environment and opening up new avenues for the development and utilization of marine biological resources.Peng LuWeinan WangGuangxi ZhangWen LiAnjie JiangMengjiao CaoXiaoyan ZhangKe XingXue PengBo YuanZhaozhong FengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 15, Iss 10, p e0240187 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Lu Weinan Wang Guangxi Zhang Wen Li Anjie Jiang Mengjiao Cao Xiaoyan Zhang Ke Xing Xue Peng Bo Yuan Zhaozhong Feng Isolation and characterization marine bacteria capable of degrading lignin-derived compounds.
description	Lignin, a characteristic component of terrestrial plants. Rivers transport large amounts of vascular plant organic matter into the oceans where lignin can degrade over time; however, microorganisms involved in this degradation have not been identified. In this study, several bacterial strains were isolated from marine samples using the lignin-derived compound vanillic acid (4-hydroxy-3-methoxybenzoic acid) as the sole carbon and energy source. The optimum growth temperature for all isolates ranged from 30 to 35°C. All isolates grew well in a wide NaCl concentration range of 0 to over 50 g/L, with an optimum concentration of 22.8 g/L, which is the same as natural seawater. Phylogenetic analysis indicates that these strains are the members of Halomonas, Arthrobacter, Pseudoalteromonas, Marinomonas, and Thalassospira. These isolates are also able to use other lignin-derived compounds, such as 4-hydroxybenzoic acid, ferulic acid, syringic acid, and benzoic acid. Vanillic acid was detected in all culture media when isolates were grown on ferulic acid as the sole carbon source; however, no 4-hydroxy-3-methoxystyrene was detected, indicating that ferulic acid metabolism by these strains occurs via the elimination of two side chain carbons. Furthermore, the isolates exhibit 3,4-dioxygenase or 4,5-dioxygenase activity for protocatechuic acid ring-cleavage, which is consistent with the genetic sequences of related genera. This study was conducted to isolate and characterize marine bacteria of degrading lignin-derived compounds, thereby revealing the degradation of aromatic compounds in the marine environment and opening up new avenues for the development and utilization of marine biological resources.
format	article
author	Peng Lu Weinan Wang Guangxi Zhang Wen Li Anjie Jiang Mengjiao Cao Xiaoyan Zhang Ke Xing Xue Peng Bo Yuan Zhaozhong Feng
author_facet	Peng Lu Weinan Wang Guangxi Zhang Wen Li Anjie Jiang Mengjiao Cao Xiaoyan Zhang Ke Xing Xue Peng Bo Yuan Zhaozhong Feng
author_sort	Peng Lu
title	Isolation and characterization marine bacteria capable of degrading lignin-derived compounds.
title_short	Isolation and characterization marine bacteria capable of degrading lignin-derived compounds.
title_full	Isolation and characterization marine bacteria capable of degrading lignin-derived compounds.
title_fullStr	Isolation and characterization marine bacteria capable of degrading lignin-derived compounds.
title_full_unstemmed	Isolation and characterization marine bacteria capable of degrading lignin-derived compounds.
title_sort	isolation and characterization marine bacteria capable of degrading lignin-derived compounds.
publisher	Public Library of Science (PLoS)
publishDate	2020
url	https://doaj.org/article/3d0692cf38cc4a68b85b0aaf0d9ca3f8
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Isolation and characterization marine bacteria capable of degrading lignin-derived compounds.

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