Volta phase plate cryo-EM of the small protein complex Prx3

Although the resolution achievable with cryo-EM can now rival crystallography, its application to small protein assemblies remains challenging. Here the authors demonstrate the use of the Volta phase plate for single particle analysis and its potential for the study of small specimens.

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Bibliographic Details
Main Authors: Maryam Khoshouei, Mazdak Radjainia, Amy J. Phillips, Juliet A. Gerrard, Alok K. Mitra, Jürgen M. Plitzko, Wolfgang Baumeister, Radostin Danev
Format: article
Language:EN
Published: Nature Portfolio 2016
Subjects:
Q
Online Access:https://doaj.org/article/3d06cce2b2344e8f80bcc7ebd49bfda0
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Summary:Although the resolution achievable with cryo-EM can now rival crystallography, its application to small protein assemblies remains challenging. Here the authors demonstrate the use of the Volta phase plate for single particle analysis and its potential for the study of small specimens.