Volta phase plate cryo-EM of the small protein complex Prx3

Although the resolution achievable with cryo-EM can now rival crystallography, its application to small protein assemblies remains challenging. Here the authors demonstrate the use of the Volta phase plate for single particle analysis and its potential for the study of small specimens.

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Autores principales: Maryam Khoshouei, Mazdak Radjainia, Amy J. Phillips, Juliet A. Gerrard, Alok K. Mitra, Jürgen M. Plitzko, Wolfgang Baumeister, Radostin Danev
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Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/3d06cce2b2344e8f80bcc7ebd49bfda0
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spelling oai:doaj.org-article:3d06cce2b2344e8f80bcc7ebd49bfda02021-12-02T17:31:33ZVolta phase plate cryo-EM of the small protein complex Prx310.1038/ncomms105342041-1723https://doaj.org/article/3d06cce2b2344e8f80bcc7ebd49bfda02016-01-01T00:00:00Zhttps://doi.org/10.1038/ncomms10534https://doaj.org/toc/2041-1723Although the resolution achievable with cryo-EM can now rival crystallography, its application to small protein assemblies remains challenging. Here the authors demonstrate the use of the Volta phase plate for single particle analysis and its potential for the study of small specimens.Maryam KhoshoueiMazdak RadjainiaAmy J. PhillipsJuliet A. GerrardAlok K. MitraJürgen M. PlitzkoWolfgang BaumeisterRadostin DanevNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-5 (2016)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Maryam Khoshouei
Mazdak Radjainia
Amy J. Phillips
Juliet A. Gerrard
Alok K. Mitra
Jürgen M. Plitzko
Wolfgang Baumeister
Radostin Danev
Volta phase plate cryo-EM of the small protein complex Prx3
description Although the resolution achievable with cryo-EM can now rival crystallography, its application to small protein assemblies remains challenging. Here the authors demonstrate the use of the Volta phase plate for single particle analysis and its potential for the study of small specimens.
format article
author Maryam Khoshouei
Mazdak Radjainia
Amy J. Phillips
Juliet A. Gerrard
Alok K. Mitra
Jürgen M. Plitzko
Wolfgang Baumeister
Radostin Danev
author_facet Maryam Khoshouei
Mazdak Radjainia
Amy J. Phillips
Juliet A. Gerrard
Alok K. Mitra
Jürgen M. Plitzko
Wolfgang Baumeister
Radostin Danev
author_sort Maryam Khoshouei
title Volta phase plate cryo-EM of the small protein complex Prx3
title_short Volta phase plate cryo-EM of the small protein complex Prx3
title_full Volta phase plate cryo-EM of the small protein complex Prx3
title_fullStr Volta phase plate cryo-EM of the small protein complex Prx3
title_full_unstemmed Volta phase plate cryo-EM of the small protein complex Prx3
title_sort volta phase plate cryo-em of the small protein complex prx3
publisher Nature Portfolio
publishDate 2016
url https://doaj.org/article/3d06cce2b2344e8f80bcc7ebd49bfda0
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