A portable library of phosphate‐depletion based synthetic promoters for customable and automata control of gene expression in bacteria

Summary Industrial biotechnology gene expression systems relay on constitutive promoters compromising cellular growth from the start of the bioprocess, or on inducible devices, which require manual addition of cognate inducers. To overcome this shortcoming, we engineered an automata regulatory syste...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jesús Torres‐Bacete, José Luís García, Juan Nogales
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/3d0a7fb9320442d9b2a47e264cd4285c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3d0a7fb9320442d9b2a47e264cd4285c
record_format	dspace
spelling	oai:doaj.org-article:3d0a7fb9320442d9b2a47e264cd4285c2021-11-18T15:39:53ZA portable library of phosphate‐depletion based synthetic promoters for customable and automata control of gene expression in bacteria1751-791510.1111/1751-7915.13808https://doaj.org/article/3d0a7fb9320442d9b2a47e264cd4285c2021-11-01T00:00:00Zhttps://doi.org/10.1111/1751-7915.13808https://doaj.org/toc/1751-7915Summary Industrial biotechnology gene expression systems relay on constitutive promoters compromising cellular growth from the start of the bioprocess, or on inducible devices, which require manual addition of cognate inducers. To overcome this shortcoming, we engineered an automata regulatory system based on cell‐stress mechanisms. Specifically, we engineered a synthetic and highly portable phosphate‐depletion library of promoters inspired by bacterial PHO starvation system (Pliar promoters). Furthermore, we fully characterized 10 synthetic promoters within the background of two well‐known bacterial workhorses such as E. coli W and P. putida KT2440. The promoters displayed an interesting host‐dependent performance and a wide strength spectrum ranging from 0.4‐ to 1.3‐fold when compared to the wild‐type phosphatase alkaline promoter (PphoA). By comparing with available gene expression systems, we proved the suitability of this new library for the automata and effective decoupling of growth from production in P. putida. Growth phase‐dependent expression of these promoters could therefore be activated by fine tuning the initial concentration of phosphate in the medium. Finally, the Pliar library was implemented in the SEVA platform in a ready‐to‐use mode allowing its broad use by the scientific community.Jesús Torres‐BaceteJosé Luís GarcíaJuan NogalesWileyarticleBiotechnologyTP248.13-248.65ENMicrobial Biotechnology, Vol 14, Iss 6, Pp 2643-2658 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Jesús Torres‐Bacete José Luís García Juan Nogales A portable library of phosphate‐depletion based synthetic promoters for customable and automata control of gene expression in bacteria
description	Summary Industrial biotechnology gene expression systems relay on constitutive promoters compromising cellular growth from the start of the bioprocess, or on inducible devices, which require manual addition of cognate inducers. To overcome this shortcoming, we engineered an automata regulatory system based on cell‐stress mechanisms. Specifically, we engineered a synthetic and highly portable phosphate‐depletion library of promoters inspired by bacterial PHO starvation system (Pliar promoters). Furthermore, we fully characterized 10 synthetic promoters within the background of two well‐known bacterial workhorses such as E. coli W and P. putida KT2440. The promoters displayed an interesting host‐dependent performance and a wide strength spectrum ranging from 0.4‐ to 1.3‐fold when compared to the wild‐type phosphatase alkaline promoter (PphoA). By comparing with available gene expression systems, we proved the suitability of this new library for the automata and effective decoupling of growth from production in P. putida. Growth phase‐dependent expression of these promoters could therefore be activated by fine tuning the initial concentration of phosphate in the medium. Finally, the Pliar library was implemented in the SEVA platform in a ready‐to‐use mode allowing its broad use by the scientific community.
format	article
author	Jesús Torres‐Bacete José Luís García Juan Nogales
author_facet	Jesús Torres‐Bacete José Luís García Juan Nogales
author_sort	Jesús Torres‐Bacete
title	A portable library of phosphate‐depletion based synthetic promoters for customable and automata control of gene expression in bacteria
title_short	A portable library of phosphate‐depletion based synthetic promoters for customable and automata control of gene expression in bacteria
title_full	A portable library of phosphate‐depletion based synthetic promoters for customable and automata control of gene expression in bacteria
title_fullStr	A portable library of phosphate‐depletion based synthetic promoters for customable and automata control of gene expression in bacteria
title_full_unstemmed	A portable library of phosphate‐depletion based synthetic promoters for customable and automata control of gene expression in bacteria
title_sort	portable library of phosphate‐depletion based synthetic promoters for customable and automata control of gene expression in bacteria
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/3d0a7fb9320442d9b2a47e264cd4285c
work_keys_str_mv	AT jesustorresbacete aportablelibraryofphosphatedepletionbasedsyntheticpromotersforcustomableandautomatacontrolofgeneexpressioninbacteria AT joseluisgarcia aportablelibraryofphosphatedepletionbasedsyntheticpromotersforcustomableandautomatacontrolofgeneexpressioninbacteria AT juannogales aportablelibraryofphosphatedepletionbasedsyntheticpromotersforcustomableandautomatacontrolofgeneexpressioninbacteria AT jesustorresbacete portablelibraryofphosphatedepletionbasedsyntheticpromotersforcustomableandautomatacontrolofgeneexpressioninbacteria AT joseluisgarcia portablelibraryofphosphatedepletionbasedsyntheticpromotersforcustomableandautomatacontrolofgeneexpressioninbacteria AT juannogales portablelibraryofphosphatedepletionbasedsyntheticpromotersforcustomableandautomatacontrolofgeneexpressioninbacteria
_version_	1718420779386798080

A portable library of phosphate‐depletion based synthetic promoters for customable and automata control of gene expression in bacteria

Ejemplares similares