A longitudinal study of amoebic gill disease on a marine Atlantic salmon farm utilising a real-time PCR assay for the detection of Neoparamoeba perurans

Amoebic gill disease (AGD) is a proliferative gill disease of marine cultured Atlantic salmon Salmo salar, with the free-living protozoan Neoparamoeba perurans being the primary aetiological agent. The increased incidence of AGD in recent years presents a significant challenge to the Atlantic salmon...

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Autores principales:	JK Downes, K Henshilwood, EM Collins, A Ryan, I O’Connor, HD Rodger, E MacCarthy, NM Ruane
Formato:	article
Lenguaje:	EN
Publicado:	Inter-Research 2015
Materias:	Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/3d0de2aaf55947558a42559098479a1a
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spelling	oai:doaj.org-article:3d0de2aaf55947558a42559098479a1a2021-11-16T09:43:56ZA longitudinal study of amoebic gill disease on a marine Atlantic salmon farm utilising a real-time PCR assay for the detection of Neoparamoeba perurans1869-215X1869-753410.3354/aei00150https://doaj.org/article/3d0de2aaf55947558a42559098479a1a2015-11-01T00:00:00Zhttps://www.int-res.com/abstracts/aei/v7/n3/p239-251/https://doaj.org/toc/1869-215Xhttps://doaj.org/toc/1869-7534Amoebic gill disease (AGD) is a proliferative gill disease of marine cultured Atlantic salmon Salmo salar, with the free-living protozoan Neoparamoeba perurans being the primary aetiological agent. The increased incidence of AGD in recent years presents a significant challenge to the Atlantic salmon farming industry in Europe. In this study, a real-time TaqMan® PCR assay was developed and validated to detect Neoparamoeba perurans on Atlantic salmon gills and further used to monitor disease progression on a marine Atlantic salmon farm in Ireland in conjunction with gross gill pathology and histopathology. The assay proved specific for N. perurans, with no cross-reactivity with the related species N. pemaquidensis, N. branchiphila or N. aestuarina, and was capable of detecting 2.68 copies of N. perurans DNA µl-1. Although the parasite was detected throughout the 18 mo period of this study, mortality peaks associated with clinical AGD were only recorded during the first 12 mo of the marine phase of the production cycle. The initial AGD outbreak resulted in peak mortality in Week 17, which was preceded by PCR detections from Week 13 onwards. Freshwater treatments were an effective method for controlling the disease, resulting in a reduction in the weekly mortality levels and also a reduction in the number of PCR-positive fish. In comparison to traditional diagnostic methods, our PCR assay proved to be highly sensitive and a valuable tool to monitor disease progression and, therefore, has the potential to provide information on the timing and effectiveness of treatments.JK DownesK HenshilwoodEM CollinsA RyanI O’ConnorHD RodgerE MacCarthyNM RuaneInter-ResearcharticleAquaculture. Fisheries. AnglingSH1-691EcologyQH540-549.5ENAquaculture Environment Interactions, Vol 7, Iss 3, Pp 239-251 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5
spellingShingle	Aquaculture. Fisheries. Angling SH1-691 Ecology QH540-549.5 JK Downes K Henshilwood EM Collins A Ryan I O’Connor HD Rodger E MacCarthy NM Ruane A longitudinal study of amoebic gill disease on a marine Atlantic salmon farm utilising a real-time PCR assay for the detection of Neoparamoeba perurans
description	Amoebic gill disease (AGD) is a proliferative gill disease of marine cultured Atlantic salmon Salmo salar, with the free-living protozoan Neoparamoeba perurans being the primary aetiological agent. The increased incidence of AGD in recent years presents a significant challenge to the Atlantic salmon farming industry in Europe. In this study, a real-time TaqMan® PCR assay was developed and validated to detect Neoparamoeba perurans on Atlantic salmon gills and further used to monitor disease progression on a marine Atlantic salmon farm in Ireland in conjunction with gross gill pathology and histopathology. The assay proved specific for N. perurans, with no cross-reactivity with the related species N. pemaquidensis, N. branchiphila or N. aestuarina, and was capable of detecting 2.68 copies of N. perurans DNA µl-1. Although the parasite was detected throughout the 18 mo period of this study, mortality peaks associated with clinical AGD were only recorded during the first 12 mo of the marine phase of the production cycle. The initial AGD outbreak resulted in peak mortality in Week 17, which was preceded by PCR detections from Week 13 onwards. Freshwater treatments were an effective method for controlling the disease, resulting in a reduction in the weekly mortality levels and also a reduction in the number of PCR-positive fish. In comparison to traditional diagnostic methods, our PCR assay proved to be highly sensitive and a valuable tool to monitor disease progression and, therefore, has the potential to provide information on the timing and effectiveness of treatments.
format	article
author	JK Downes K Henshilwood EM Collins A Ryan I O’Connor HD Rodger E MacCarthy NM Ruane
author_facet	JK Downes K Henshilwood EM Collins A Ryan I O’Connor HD Rodger E MacCarthy NM Ruane
author_sort	JK Downes
title	A longitudinal study of amoebic gill disease on a marine Atlantic salmon farm utilising a real-time PCR assay for the detection of Neoparamoeba perurans
title_short	A longitudinal study of amoebic gill disease on a marine Atlantic salmon farm utilising a real-time PCR assay for the detection of Neoparamoeba perurans
title_full	A longitudinal study of amoebic gill disease on a marine Atlantic salmon farm utilising a real-time PCR assay for the detection of Neoparamoeba perurans
title_fullStr	A longitudinal study of amoebic gill disease on a marine Atlantic salmon farm utilising a real-time PCR assay for the detection of Neoparamoeba perurans
title_full_unstemmed	A longitudinal study of amoebic gill disease on a marine Atlantic salmon farm utilising a real-time PCR assay for the detection of Neoparamoeba perurans
title_sort	longitudinal study of amoebic gill disease on a marine atlantic salmon farm utilising a real-time pcr assay for the detection of neoparamoeba perurans
publisher	Inter-Research
publishDate	2015
url	https://doaj.org/article/3d0de2aaf55947558a42559098479a1a
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A longitudinal study of amoebic gill disease on a marine Atlantic salmon farm utilising a real-time PCR assay for the detection of Neoparamoeba perurans

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