Development of SimCells as a novel chassis for functional biosensors

Abstract This work serves as a proof-of-concept for bacterially derived SimCells (Simple Cells), which contain the cell machinery from bacteria and designed DNA (or potentially a simplified genome) to instruct the cell to carry out novel, specific tasks. SimCells represent a reprogrammable chassis w...

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Autores principales:	Cordelia P. N. Rampley, Paul A. Davison, Pu Qian, Gail M. Preston, C. Neil Hunter, Ian P. Thompson, Ling Juan Wu, Wei E. Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3d0fd9a8624f4df2992b9b31e8e450c3
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spelling	oai:doaj.org-article:3d0fd9a8624f4df2992b9b31e8e450c32021-12-02T15:05:41ZDevelopment of SimCells as a novel chassis for functional biosensors10.1038/s41598-017-07391-62045-2322https://doaj.org/article/3d0fd9a8624f4df2992b9b31e8e450c32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07391-6https://doaj.org/toc/2045-2322Abstract This work serves as a proof-of-concept for bacterially derived SimCells (Simple Cells), which contain the cell machinery from bacteria and designed DNA (or potentially a simplified genome) to instruct the cell to carry out novel, specific tasks. SimCells represent a reprogrammable chassis without a native chromosome, which can host designed DNA to perform defined functions. In this paper, the use of Escherichia coli MC1000 ∆minD minicells as a non-reproducing chassis for SimCells was explored, as demonstrated by their ability to act as sensitive biosensors for small molecules. Highly purified minicells derived from E. coli strains containing gene circuits for biosensing were able to transduce the input signals from several small molecules (glucarate, acrylate and arabinose) into the production of green fluorescent protein (GFP). A mathematical model was developed to fit the experimental data for induction of gene expression in SimCells. The intracellular ATP level was shown to be important for SimCell function. A purification and storage protocol was developed to prepare SimCells which could retain their functions for an extended period of time. This study demonstrates that SimCells are able to perform as ‘smart bioparticles’ controlled by designed gene circuits.Cordelia P. N. RampleyPaul A. DavisonPu QianGail M. PrestonC. Neil HunterIan P. ThompsonLing Juan WuWei E. HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cordelia P. N. Rampley Paul A. Davison Pu Qian Gail M. Preston C. Neil Hunter Ian P. Thompson Ling Juan Wu Wei E. Huang Development of SimCells as a novel chassis for functional biosensors
description	Abstract This work serves as a proof-of-concept for bacterially derived SimCells (Simple Cells), which contain the cell machinery from bacteria and designed DNA (or potentially a simplified genome) to instruct the cell to carry out novel, specific tasks. SimCells represent a reprogrammable chassis without a native chromosome, which can host designed DNA to perform defined functions. In this paper, the use of Escherichia coli MC1000 ∆minD minicells as a non-reproducing chassis for SimCells was explored, as demonstrated by their ability to act as sensitive biosensors for small molecules. Highly purified minicells derived from E. coli strains containing gene circuits for biosensing were able to transduce the input signals from several small molecules (glucarate, acrylate and arabinose) into the production of green fluorescent protein (GFP). A mathematical model was developed to fit the experimental data for induction of gene expression in SimCells. The intracellular ATP level was shown to be important for SimCell function. A purification and storage protocol was developed to prepare SimCells which could retain their functions for an extended period of time. This study demonstrates that SimCells are able to perform as ‘smart bioparticles’ controlled by designed gene circuits.
format	article
author	Cordelia P. N. Rampley Paul A. Davison Pu Qian Gail M. Preston C. Neil Hunter Ian P. Thompson Ling Juan Wu Wei E. Huang
author_facet	Cordelia P. N. Rampley Paul A. Davison Pu Qian Gail M. Preston C. Neil Hunter Ian P. Thompson Ling Juan Wu Wei E. Huang
author_sort	Cordelia P. N. Rampley
title	Development of SimCells as a novel chassis for functional biosensors
title_short	Development of SimCells as a novel chassis for functional biosensors
title_full	Development of SimCells as a novel chassis for functional biosensors
title_fullStr	Development of SimCells as a novel chassis for functional biosensors
title_full_unstemmed	Development of SimCells as a novel chassis for functional biosensors
title_sort	development of simcells as a novel chassis for functional biosensors
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3d0fd9a8624f4df2992b9b31e8e450c3
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_version_	1718388697905233920

Development of SimCells as a novel chassis for functional biosensors

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