The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction

The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction

Wenbin Cai,1,2,* Wei Lv,1,3,* Yang Feng,4 Hengli Yang,1 Yajun Zhang,1 Guodong Yang,5 Yunyou Duan,1 Jia Wang1 1Department of Ultrasound Diagnosis, Tang Du Hospital, Fourth Military Medical University, Xi’an 710038, China; 2General Hospital of Tibet Military Command, Lhasa, Tibet Autonomous...

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Autores principales: Cai W, Lv W, Feng Y, Yang H, Zhang Y, Yang G, Duan Y, Wang J
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2018
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Nanobubbles Ultrasound siRNA Glioma
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/3d2ce7e340e14a43b706d39e0b876954
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spelling oai:doaj.org-article:3d2ce7e340e14a43b706d39e0b8769542021-12-02T06:14:11ZThe therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction1178-2013https://doaj.org/article/3d2ce7e340e14a43b706d39e0b8769542018-10-01T00:00:00Zhttps://www.dovepress.com/the-therapeutic-effect-in-gliomas-of-nanobubbles-carrying-sirna-combin-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wenbin Cai,1,2,* Wei Lv,1,3,* Yang Feng,4 Hengli Yang,1 Yajun Zhang,1 Guodong Yang,5 Yunyou Duan,1 Jia Wang1 1Department of Ultrasound Diagnosis, Tang Du Hospital, Fourth Military Medical University, Xi’an 710038, China; 2General Hospital of Tibet Military Command, Lhasa, Tibet Autonomous Region, 850007, China; 3Department of Radiology, 305 Hospital of Chinese People’s Liberation Army, Xicheng District, Beijing, 100017, China; 4Xijing Hospital, Traditional Chinese Medicine, Xi’an 710032, China; 5Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi’an 710032, China *These authors contributed equally to this work Background: Nanobubbles (NBs) combined with ultrasound-targeted destruction (UTD) have become promising potential carriers for drug or siRNA delivery. Due to their nano-size, NBs could penetrate tumor blood vessels and accumulate in intercellular spaces so that “sonoporation” induced by UTD would act directly on the tumor cells to increase cell membrane permeability. Methods: Based on the successful the fabrication of NBs, we synthesized NBs carrying siRNA (NBs-siRNA) by using a biotin-streptavidin system. We then utilized ultrasound irradiation (UI)-targeted NBs-siRNA to improve siRNA transfection and achieve the inhibition of glioma growth.Results: NBs as carriers combined with UI effectively enhanced siRNA transfection and the effect of silencing targeted genes in vitro. Additionally, a better therapeutic effect was shown in the NBs-siRNA with UI group in vivo compared with that of microbubbles (MBs) with UI or NBs-siRNA without UI.Conclusion: These results indicated that NBs combined with UTD might be an ideal delivery vector for siRNA to achieve the noninvasive treatment of glioma. Keywords: nanobubbles, ultrasound, siRNA, gliomaCai WLv WFeng YYang HZhang YYang GDuan YWang JDove Medical PressarticleNanobubbles Ultrasound siRNA GliomaMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 6791-6807 (2018)
institution DOAJ
collection DOAJ
language EN
topic Nanobubbles Ultrasound siRNA Glioma
Medicine (General)
R5-920
spellingShingle Nanobubbles Ultrasound siRNA Glioma
Medicine (General)
R5-920
Cai W
Lv W
Feng Y
Yang H
Zhang Y
Yang G
Duan Y
Wang J
The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
description Wenbin Cai,1,2,* Wei Lv,1,3,* Yang Feng,4 Hengli Yang,1 Yajun Zhang,1 Guodong Yang,5 Yunyou Duan,1 Jia Wang1 1Department of Ultrasound Diagnosis, Tang Du Hospital, Fourth Military Medical University, Xi’an 710038, China; 2General Hospital of Tibet Military Command, Lhasa, Tibet Autonomous Region, 850007, China; 3Department of Radiology, 305 Hospital of Chinese People’s Liberation Army, Xicheng District, Beijing, 100017, China; 4Xijing Hospital, Traditional Chinese Medicine, Xi’an 710032, China; 5Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi’an 710032, China *These authors contributed equally to this work Background: Nanobubbles (NBs) combined with ultrasound-targeted destruction (UTD) have become promising potential carriers for drug or siRNA delivery. Due to their nano-size, NBs could penetrate tumor blood vessels and accumulate in intercellular spaces so that “sonoporation” induced by UTD would act directly on the tumor cells to increase cell membrane permeability. Methods: Based on the successful the fabrication of NBs, we synthesized NBs carrying siRNA (NBs-siRNA) by using a biotin-streptavidin system. We then utilized ultrasound irradiation (UI)-targeted NBs-siRNA to improve siRNA transfection and achieve the inhibition of glioma growth.Results: NBs as carriers combined with UI effectively enhanced siRNA transfection and the effect of silencing targeted genes in vitro. Additionally, a better therapeutic effect was shown in the NBs-siRNA with UI group in vivo compared with that of microbubbles (MBs) with UI or NBs-siRNA without UI.Conclusion: These results indicated that NBs combined with UTD might be an ideal delivery vector for siRNA to achieve the noninvasive treatment of glioma. Keywords: nanobubbles, ultrasound, siRNA, glioma
format article
author Cai W
Lv W
Feng Y
Yang H
Zhang Y
Yang G
Duan Y
Wang J
author_facet Cai W
Lv W
Feng Y
Yang H
Zhang Y
Yang G
Duan Y
Wang J
author_sort Cai W
title The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title_short The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title_full The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title_fullStr The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title_full_unstemmed The therapeutic effect in gliomas of nanobubbles carrying siRNA combined with ultrasound-targeted destruction
title_sort therapeutic effect in gliomas of nanobubbles carrying sirna combined with ultrasound-targeted destruction
publisher Dove Medical Press
publishDate 2018
url https://doaj.org/article/3d2ce7e340e14a43b706d39e0b876954
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