POU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis

Abdominal aortic aneurysm (AAA) is life-threatening, its natural course is progressively sac expansion and rupture. Elegant studies have been conducted to investigate the molecular markers associated with AAA growth and expansion, this topic however, still needs to be further elucidated. This study...

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Autores principales:	Jinze Meng, Hao Wen, Xintong Li, Boyang Luan, Shiqiang Gong, Jie Wen, Yifei Wang, Lei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	abdominal aortic aneurysm signed weighted gene co-expression network analysis protein-protein interaction differentially expressed gene analysis pou class 2 homeobox associating factor 1 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/3d2d7163d48f4670b8e7a7012152a291
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spelling	oai:doaj.org-article:3d2d7163d48f4670b8e7a7012152a2912021-11-04T15:51:54ZPOU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis2165-59792165-598710.1080/21655979.2021.1990822https://doaj.org/article/3d2d7163d48f4670b8e7a7012152a2912021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1990822https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Abdominal aortic aneurysm (AAA) is life-threatening, its natural course is progressively sac expansion and rupture. Elegant studies have been conducted to investigate the molecular markers associated with AAA growth and expansion, this topic however, still needs to be further elucidated. This study aimed to identify potential genes for AAA growth and expansion based on comprehensive bioinformatics approaches. Firstly, 29 up-regulated genes were identified through DEGs analysis between large AAA and small AAA in GSE57691. Secondly, signed WGCNA analysis was conducted based on GSE57691 and the green module was found to exhibit the topmost correlation with large AAA as well as AAA, 133 WGCNA hub genes were further identified. Merged gene set including 29 up-regulated DEGs and 858 green module genes was subjected to constructing a PPI network where 195 PPI hub genes were identified. Subsequently, 4 crucial genes including POU2AF1, FCRLA, CD79B, HLA-DOB were recognized by Venn plot. In addition, by using GSE7084 and GSE98278 for verification, POU2AF1 showed potential diagnostic value between AAA and normal groups, and exhibited a significant higher expression level in large AAA samples compared with small AAA samples. Furthermore, immunohistochemistry results indicated up-regulation of POU2AF1 in large AAA samples than small AAA samples, which implies POU2AF1 may be a key regulator in AAA enlargement and growth. In summary, this study indicates that POU2AF1 has great predictive value for the expansion of AAA, and may contribute to the further exploration of pathogenesis and progression of AAA.Jinze MengHao WenXintong LiBoyang LuanShiqiang GongJie WenYifei WangLei WangTaylor & Francis Grouparticleabdominal aortic aneurysmsigned weighted gene co-expression network analysisprotein-protein interactiondifferentially expressed gene analysispou class 2 homeobox associating factor 1BiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8980-8993 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	abdominal aortic aneurysm signed weighted gene co-expression network analysis protein-protein interaction differentially expressed gene analysis pou class 2 homeobox associating factor 1 Biotechnology TP248.13-248.65
spellingShingle	abdominal aortic aneurysm signed weighted gene co-expression network analysis protein-protein interaction differentially expressed gene analysis pou class 2 homeobox associating factor 1 Biotechnology TP248.13-248.65 Jinze Meng Hao Wen Xintong Li Boyang Luan Shiqiang Gong Jie Wen Yifei Wang Lei Wang POU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis
description	Abdominal aortic aneurysm (AAA) is life-threatening, its natural course is progressively sac expansion and rupture. Elegant studies have been conducted to investigate the molecular markers associated with AAA growth and expansion, this topic however, still needs to be further elucidated. This study aimed to identify potential genes for AAA growth and expansion based on comprehensive bioinformatics approaches. Firstly, 29 up-regulated genes were identified through DEGs analysis between large AAA and small AAA in GSE57691. Secondly, signed WGCNA analysis was conducted based on GSE57691 and the green module was found to exhibit the topmost correlation with large AAA as well as AAA, 133 WGCNA hub genes were further identified. Merged gene set including 29 up-regulated DEGs and 858 green module genes was subjected to constructing a PPI network where 195 PPI hub genes were identified. Subsequently, 4 crucial genes including POU2AF1, FCRLA, CD79B, HLA-DOB were recognized by Venn plot. In addition, by using GSE7084 and GSE98278 for verification, POU2AF1 showed potential diagnostic value between AAA and normal groups, and exhibited a significant higher expression level in large AAA samples compared with small AAA samples. Furthermore, immunohistochemistry results indicated up-regulation of POU2AF1 in large AAA samples than small AAA samples, which implies POU2AF1 may be a key regulator in AAA enlargement and growth. In summary, this study indicates that POU2AF1 has great predictive value for the expansion of AAA, and may contribute to the further exploration of pathogenesis and progression of AAA.
format	article
author	Jinze Meng Hao Wen Xintong Li Boyang Luan Shiqiang Gong Jie Wen Yifei Wang Lei Wang
author_facet	Jinze Meng Hao Wen Xintong Li Boyang Luan Shiqiang Gong Jie Wen Yifei Wang Lei Wang
author_sort	Jinze Meng
title	POU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis
title_short	POU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis
title_full	POU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis
title_fullStr	POU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis
title_full_unstemmed	POU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis
title_sort	pou class 2 homeobox associating factor 1 (pou2af1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/3d2d7163d48f4670b8e7a7012152a291
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POU class 2 homeobox associating factor 1 (POU2AF1) participates in abdominal aortic aneurysm enlargement based on integrated bioinformatics analysis

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