DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line

Abstract. Angkat NU, Siregar LAM, Basyuni M, Afandi D, Syahputra I. 2021. DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line. Biodiversitas 22: 900-905. The acidification of palm oil due to lipase activity in the mesocarp is assessed under genet...

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Autores principales:	Nina Unzila Angkat, LUTHFI AZIZ MAHMUD SIREGAR, Mohammad Basyuni, Dadang Afandi, INDRA SYAHPUTRA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	amova, low lipase, microsatellite, oil palm Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3d2e585c190e46d8b485a23967ca3914
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spelling	oai:doaj.org-article:3d2e585c190e46d8b485a23967ca39142021-11-22T00:54:56ZDNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line1412-033X2085-472210.13057/biodiv/d220245https://doaj.org/article/3d2e585c190e46d8b485a23967ca39142021-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6897https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Angkat NU, Siregar LAM, Basyuni M, Afandi D, Syahputra I. 2021. DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line. Biodiversitas 22: 900-905. The acidification of palm oil due to lipase activity in the mesocarp is assessed under genetic control. Three molecular markers have been established to gauge the lipase gene in oil palm. Lower lipase activity is desired for good quality edible oil. This study aims to identify the genetic diversity by screening groups/families to determine an elite low lipase genotype of oil palm. Genetic diversity and population structure of 15 groups of oil palm were investigated by using three specific markers with GenAlex 6.502 software. Results show that the Polymorphic Informative Content (PIC) value of markers was around 0,985-0,993, which indicates that these markers are effective in determining the diversity of lipase activity in oil palm. Analysis of molecular variance (AMOVA) revealed that genetic diversity varies within individuals (54%), among individuals (31%), and among population (15%). The value of number of alleles (Na), number of effective alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and number of allele migration (Nm) indicate that the genetic diversity in this population is relatively low. Phylogenetic analysis identified two main groups as high lipase and low lipase activity groups based on DNA intensity.Nina Unzila AngkatLUTHFI AZIZ MAHMUD SIREGARMohammad BasyuniDadang AfandiINDRA SYAHPUTRAMBI & UNS Soloarticleamova, low lipase, microsatellite, oil palmBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	amova, low lipase, microsatellite, oil palm Biology (General) QH301-705.5
spellingShingle	amova, low lipase, microsatellite, oil palm Biology (General) QH301-705.5 Nina Unzila Angkat LUTHFI AZIZ MAHMUD SIREGAR Mohammad Basyuni Dadang Afandi INDRA SYAHPUTRA DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line
description	Abstract. Angkat NU, Siregar LAM, Basyuni M, Afandi D, Syahputra I. 2021. DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line. Biodiversitas 22: 900-905. The acidification of palm oil due to lipase activity in the mesocarp is assessed under genetic control. Three molecular markers have been established to gauge the lipase gene in oil palm. Lower lipase activity is desired for good quality edible oil. This study aims to identify the genetic diversity by screening groups/families to determine an elite low lipase genotype of oil palm. Genetic diversity and population structure of 15 groups of oil palm were investigated by using three specific markers with GenAlex 6.502 software. Results show that the Polymorphic Informative Content (PIC) value of markers was around 0,985-0,993, which indicates that these markers are effective in determining the diversity of lipase activity in oil palm. Analysis of molecular variance (AMOVA) revealed that genetic diversity varies within individuals (54%), among individuals (31%), and among population (15%). The value of number of alleles (Na), number of effective alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and number of allele migration (Nm) indicate that the genetic diversity in this population is relatively low. Phylogenetic analysis identified two main groups as high lipase and low lipase activity groups based on DNA intensity.
format	article
author	Nina Unzila Angkat LUTHFI AZIZ MAHMUD SIREGAR Mohammad Basyuni Dadang Afandi INDRA SYAHPUTRA
author_facet	Nina Unzila Angkat LUTHFI AZIZ MAHMUD SIREGAR Mohammad Basyuni Dadang Afandi INDRA SYAHPUTRA
author_sort	Nina Unzila Angkat
title	DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line
title_short	DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line
title_full	DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line
title_fullStr	DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line
title_full_unstemmed	DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line
title_sort	dna intensity and genetic diversity of oil palm (elaeis guineensis) to determine an elite low lipase line
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/3d2e585c190e46d8b485a23967ca3914
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DNA intensity and genetic diversity of oil palm (Elaeis guineensis) to determine an elite low lipase line

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